Yulan Sun, Daitao Zhang, Hui Liu, Chunlai Ruan, Xiangfeng Dou, Zhenyong Ren, Ziruo Ge, Zhizhong Du, Haoyuan Jin, Dan Li, Hui Xue, Wei Liu, Zhihai Chen, Quanyi Wang
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The SFTS virus was successfully isolated from both patient and camel samples. The complete nucleotide sequences obtained from the infected patients demonstrated a remarkable 100% similarity to those found in the camel, and genetic evolution analysis classified the virus as genotype A. Additionally, partial sequences of the SFTS virus were identified in ticks captured from the camel rearing environment, however, these sequences showed only 95.9% similarity to those found in camel and humans. Furthermore, immunoglobulin M and immunoglobulin G antibodies were detected in serum samples collected from the patient. Our findings provide evidence that camel may serve as a competent reservoir for transmitting the SFTS virus to humans. 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引用次数: 0
摘要
摘要严重发热伴血小板减少综合征(SFTS)是一种新出现的蜱媒疾病,发病率逐年上升。在本病例报告中,我们介绍了两名感染 SFTS 病毒的患者,这表明骆驼可能通过血液接触直接传播给人类。这两名患者都是在参与宰杀一头患病骆驼后出现症状的,而他们生活在同一环境中的家人或共同宰杀骆驼的人却未受影响。随后的检测发现,从患病骆驼身上获取的样本中含有大量 SFTS 病毒,病毒 RNA 拷贝数高达 1010 个/毫升。元基因组测序没有发现其他病原体。从病人和骆驼样本中都成功分离出了 SFTS 病毒。从受感染患者体内获得的完整核苷酸序列与骆驼体内发现的核苷酸序列相似度高达 100%,遗传进化分析将该病毒归为 A 基因型。此外,从骆驼饲养环境中捕获的蜱虫体内也发现了 SFTS 病毒的部分序列,但这些序列与骆驼和人类体内发现的序列相似度仅为 95.9%。此外,从患者采集的血清样本中检测到了免疫球蛋白 M 和免疫球蛋白 G 抗体。我们的研究结果提供了证据,证明骆驼可能是将 SFTS 病毒传播给人类的合格储库。有必要对大型动物的 SFTS 病毒感染进行进一步的体外研究,以了解它们在病毒维持和传播中的作用。
The first reported cases of severe fever with thrombocytopenia syndrome virus from domestic sick camel to humans in China.
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease with an increasing annual incidence rate. In this case report, we presented two patients infected with the SFTS virus, suggesting a potential direct transmission route from camels to humans through blood contact. Both patients developed symptoms after engaging in the slaughtering of one sick camel, while their family members living in the same environment or co-diners remained unaffected. Subsequent detection revealed a high viral load of SFTS virus, reaching 1010 viral RNA copies/ml, in the sample obtained from the sick camel. Metagenomic sequencing did not identify any other pathogens. The SFTS virus was successfully isolated from both patient and camel samples. The complete nucleotide sequences obtained from the infected patients demonstrated a remarkable 100% similarity to those found in the camel, and genetic evolution analysis classified the virus as genotype A. Additionally, partial sequences of the SFTS virus were identified in ticks captured from the camel rearing environment, however, these sequences showed only 95.9% similarity to those found in camel and humans. Furthermore, immunoglobulin M and immunoglobulin G antibodies were detected in serum samples collected from the patient. Our findings provide evidence that camel may serve as a competent reservoir for transmitting the SFTS virus to humans. Further in vitro investigations into SFTS virus infections in large animals are warranted to understand their role in viral maintenance and transmission.
期刊介绍:
Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses.
The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries.
This journal addresses topics of critical biological and clinical importance, including but not limited to:
- Epidemic surveillance
- Clinical manifestations
- Diagnosis and management
- Cellular and molecular pathogenesis
- Innate and acquired immune responses between emerging microbes and their hosts
- Drug discovery
- Vaccine development research
Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.