Izaías Araújo de Oliveira, Luciane de Nazaré Almeida dos Reis, Flávia Milene Barros Nery, Niday Alline Nunes Fernandes, Vinícius Peres Machado Reis Gonçalves, Maria R. Rojas, Robert L. Gilbertson, Ailton Reis, Leonardo Silva Boiteux, Maria Esther de Noronha Fonseca-Boiteux, Rita de Cássia Pereira-Carvalho
{"title":"巴西亚马逊河流域高度分化的番茄萎黄斑驳盖因病毒株的基因组特征","authors":"Izaías Araújo de Oliveira, Luciane de Nazaré Almeida dos Reis, Flávia Milene Barros Nery, Niday Alline Nunes Fernandes, Vinícius Peres Machado Reis Gonçalves, Maria R. Rojas, Robert L. Gilbertson, Ailton Reis, Leonardo Silva Boiteux, Maria Esther de Noronha Fonseca-Boiteux, Rita de Cássia Pereira-Carvalho","doi":"10.1007/s12600-024-01122-0","DOIUrl":null,"url":null,"abstract":"<p>Foliar samples were collected from the tomato (<i>Solanum lycopersicum</i>) cultivar ‘IPA-6’ exhibiting begomovirus-like symptoms (apical leaf curling and chlorosis) in Iranduba (Brazilian Amazon). PCR tests with degenerate primers targeting DNA–A and DNA–B components of a sample designated as AM–035 were positive for both genomic regions. Full-length cognate DNA–A and DNA–B components of AM–035 were cloned and sequenced via Sanger dideoxy termination reaction. The complete sequence of the DNA–A component (MK878452) comprised 2,630 nucleotides (organized in five open reading frames – ORFs), and the DNA–B component (MK878451) displayed 2,593 nucleotides (two ORFs). The highest identities ranged from 90 to 92% with tomato chlorotic mottle Guyane virus (ToCMoGV) isolates from French Guiana, indicating a highly divergent Brazilian strain of this virus. The present work reinforces the scenario of the high diversity of tomato-infecting begomoviruses in Brazil. Further studies are needed to determine the distribution and prevalence of ToCMoGV across tomato-producing areas in the Brazilian Amazon River Basin.</p>","PeriodicalId":20220,"journal":{"name":"Phytoparasitica","volume":"1 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genomic characterization of a highly divergent tomato chlorotic mottle Guyane virus strain in the Brazilian Amazon River Basin\",\"authors\":\"Izaías Araújo de Oliveira, Luciane de Nazaré Almeida dos Reis, Flávia Milene Barros Nery, Niday Alline Nunes Fernandes, Vinícius Peres Machado Reis Gonçalves, Maria R. Rojas, Robert L. Gilbertson, Ailton Reis, Leonardo Silva Boiteux, Maria Esther de Noronha Fonseca-Boiteux, Rita de Cássia Pereira-Carvalho\",\"doi\":\"10.1007/s12600-024-01122-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Foliar samples were collected from the tomato (<i>Solanum lycopersicum</i>) cultivar ‘IPA-6’ exhibiting begomovirus-like symptoms (apical leaf curling and chlorosis) in Iranduba (Brazilian Amazon). PCR tests with degenerate primers targeting DNA–A and DNA–B components of a sample designated as AM–035 were positive for both genomic regions. Full-length cognate DNA–A and DNA–B components of AM–035 were cloned and sequenced via Sanger dideoxy termination reaction. The complete sequence of the DNA–A component (MK878452) comprised 2,630 nucleotides (organized in five open reading frames – ORFs), and the DNA–B component (MK878451) displayed 2,593 nucleotides (two ORFs). The highest identities ranged from 90 to 92% with tomato chlorotic mottle Guyane virus (ToCMoGV) isolates from French Guiana, indicating a highly divergent Brazilian strain of this virus. The present work reinforces the scenario of the high diversity of tomato-infecting begomoviruses in Brazil. Further studies are needed to determine the distribution and prevalence of ToCMoGV across tomato-producing areas in the Brazilian Amazon River Basin.</p>\",\"PeriodicalId\":20220,\"journal\":{\"name\":\"Phytoparasitica\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytoparasitica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12600-024-01122-0\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytoparasitica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12600-024-01122-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Genomic characterization of a highly divergent tomato chlorotic mottle Guyane virus strain in the Brazilian Amazon River Basin
Foliar samples were collected from the tomato (Solanum lycopersicum) cultivar ‘IPA-6’ exhibiting begomovirus-like symptoms (apical leaf curling and chlorosis) in Iranduba (Brazilian Amazon). PCR tests with degenerate primers targeting DNA–A and DNA–B components of a sample designated as AM–035 were positive for both genomic regions. Full-length cognate DNA–A and DNA–B components of AM–035 were cloned and sequenced via Sanger dideoxy termination reaction. The complete sequence of the DNA–A component (MK878452) comprised 2,630 nucleotides (organized in five open reading frames – ORFs), and the DNA–B component (MK878451) displayed 2,593 nucleotides (two ORFs). The highest identities ranged from 90 to 92% with tomato chlorotic mottle Guyane virus (ToCMoGV) isolates from French Guiana, indicating a highly divergent Brazilian strain of this virus. The present work reinforces the scenario of the high diversity of tomato-infecting begomoviruses in Brazil. Further studies are needed to determine the distribution and prevalence of ToCMoGV across tomato-producing areas in the Brazilian Amazon River Basin.
期刊介绍：
Phytoparasitica is an international journal on Plant Protection, that publishes original research contributions on the biological, chemical and molecular aspects of Entomology, Plant Pathology, Virology, Nematology, and Weed Sciences, which strives to improve scientific knowledge and technology for IPM, in forest and agroecosystems. Phytoparasitica emphasizes new insights into plant disease and pest etiology, epidemiology, host-parasite/pest biochemistry and cell biology, ecology and population biology, host genetics and resistance, disease vector biology, plant stress and biotic disorders, postharvest pathology and mycotoxins. Research can cover aspects related to the nature of plant diseases, pests and weeds, the causal agents, their spread, the losses they cause, crop loss assessment, and novel tactics and approaches for their management.
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