VAR2 基因突变是蝴蝶兰 "崇光薇薇安 "叶片变异的原因之一

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences Horticulture Environment and Biotechnology Pub Date : 2024-01-23 DOI:10.1007/s13580-023-00543-w
Febri Yuda Kurniawan, Saifa Usni Putri, Aviesta Linggabuwana, Muhammad Dylan Lawrie, Aziz Purwantoro, Endang Semiarti
{"title":"VAR2 基因突变是蝴蝶兰 \"崇光薇薇安 \"叶片变异的原因之一","authors":"Febri Yuda Kurniawan, Saifa Usni Putri, Aviesta Linggabuwana, Muhammad Dylan Lawrie, Aziz Purwantoro, Endang Semiarti","doi":"10.1007/s13580-023-00543-w","DOIUrl":null,"url":null,"abstract":"<p><i>Phalaenopsis</i> ‘Sogo Vivien’ is a hybrid orchid that can produce a mutant with foliar variegation. Variegation can be divided into structural-type and pigment-type variegation. Foliar variegation was mostly caused by mutations in plastid genes. Even though there are various studies on foliar variegation in other plants, studies on foliar variegation in orchids are quite limited. This study aimed to determine the variegated leaf phenotype characteristics and their cause in <i>P</i>. ‘Sogo Vivien’. This study observed the anatomy of variegated leaves by comparing the green and white parts of the leaf, measured the concentration of chlorophyll pigments, and detected leaf starch content between both parts. In addition, polymorphism analysis was also performed on the <i>VAR2</i> gene partial sequences. Based on leaf anatomy structure, the difference between variegated zones is only in the number of chloroplasts, the diameter of parenchyma cells, and chloroplasts. The chloroplast diameter within the white part is smaller than the green part. The chloroplasts’ diameters in the green part are two times larger than the white part, with four times more in amount. The two parts were formed due to differences in the distribution pattern of functional chloroplasts in the leaves. The concentration of chlorophyll in the green part is higher than in the white part, a characteristic of the chlorophyll-type variegation. The green parts have more starch grains than the white parts. Stomata distance between variegated zones on the abaxial side is different, and it was suggested as a distinctive character between variegated zones. A thymine insertion in exon 1 contributed to nonsense and missense mutations on the <i>VAR2</i> gene isolated from the white part. This mutation caused the amino acid motif to change from KAKFQ to KA—Q. This mutation is suggested to cause the variegated leaf phenomenon in <i>P</i>. ‘Sogo Vivien’.</p>","PeriodicalId":13123,"journal":{"name":"Horticulture Environment and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mutation of the VAR2 gene plays a role as one of the causes of leaf variegation in the moth orchid Phalaenopsis ‘Sogo Vivien’\",\"authors\":\"Febri Yuda Kurniawan, Saifa Usni Putri, Aviesta Linggabuwana, Muhammad Dylan Lawrie, Aziz Purwantoro, Endang Semiarti\",\"doi\":\"10.1007/s13580-023-00543-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Phalaenopsis</i> ‘Sogo Vivien’ is a hybrid orchid that can produce a mutant with foliar variegation. Variegation can be divided into structural-type and pigment-type variegation. Foliar variegation was mostly caused by mutations in plastid genes. Even though there are various studies on foliar variegation in other plants, studies on foliar variegation in orchids are quite limited. This study aimed to determine the variegated leaf phenotype characteristics and their cause in <i>P</i>. ‘Sogo Vivien’. This study observed the anatomy of variegated leaves by comparing the green and white parts of the leaf, measured the concentration of chlorophyll pigments, and detected leaf starch content between both parts. In addition, polymorphism analysis was also performed on the <i>VAR2</i> gene partial sequences. Based on leaf anatomy structure, the difference between variegated zones is only in the number of chloroplasts, the diameter of parenchyma cells, and chloroplasts. The chloroplast diameter within the white part is smaller than the green part. The chloroplasts’ diameters in the green part are two times larger than the white part, with four times more in amount. The two parts were formed due to differences in the distribution pattern of functional chloroplasts in the leaves. The concentration of chlorophyll in the green part is higher than in the white part, a characteristic of the chlorophyll-type variegation. The green parts have more starch grains than the white parts. Stomata distance between variegated zones on the abaxial side is different, and it was suggested as a distinctive character between variegated zones. A thymine insertion in exon 1 contributed to nonsense and missense mutations on the <i>VAR2</i> gene isolated from the white part. This mutation caused the amino acid motif to change from KAKFQ to KA—Q. This mutation is suggested to cause the variegated leaf phenomenon in <i>P</i>. ‘Sogo Vivien’.</p>\",\"PeriodicalId\":13123,\"journal\":{\"name\":\"Horticulture Environment and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-01-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticulture Environment and Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s13580-023-00543-w\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticulture Environment and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s13580-023-00543-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

摘要

蝴蝶兰'崇光薇薇安'是一种杂交兰花,可以产生叶片变色的突变体。变色可分为结构型变色和色素型变色。叶片变色主要是由质体基因突变引起的。尽管对其他植物的叶片变色有各种研究,但对兰花叶片变色的研究却相当有限。本研究旨在确定'崇光维维安'的叶片变异表型特征及其原因。本研究通过比较叶片的绿色部分和白色部分,观察了变色叶片的解剖结构,测量了叶绿素色素的浓度,并检测了两部分叶片的淀粉含量。此外,还对 VAR2 基因部分序列进行了多态性分析。从叶片解剖结构来看,变色区之间的差异仅表现在叶绿体数量、实质细胞直径和叶绿体上。白色部分的叶绿体直径小于绿色部分。绿色部分的叶绿体直径是白色部分的 2 倍,数量是白色部分的 4 倍。这两个部分的形成是由于功能叶绿体在叶片中的分布模式不同。绿色部分的叶绿素浓度高于白色部分,这是叶绿素型变异的特征。绿色部分的淀粉粒比白色部分多。背面变色区之间的气孔距离不同,这被认为是变色区之间的一个显著特征。外显子 1 中的胸腺嘧啶插入导致从白色部分分离出来的 VAR2 基因发生无义和错义突变。该突变导致氨基酸基序从 KAKFQ 变为 KA-Q。这种突变被认为是造成'崇光薇薇安'变色叶现象的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Mutation of the VAR2 gene plays a role as one of the causes of leaf variegation in the moth orchid Phalaenopsis ‘Sogo Vivien’

Phalaenopsis ‘Sogo Vivien’ is a hybrid orchid that can produce a mutant with foliar variegation. Variegation can be divided into structural-type and pigment-type variegation. Foliar variegation was mostly caused by mutations in plastid genes. Even though there are various studies on foliar variegation in other plants, studies on foliar variegation in orchids are quite limited. This study aimed to determine the variegated leaf phenotype characteristics and their cause in P. ‘Sogo Vivien’. This study observed the anatomy of variegated leaves by comparing the green and white parts of the leaf, measured the concentration of chlorophyll pigments, and detected leaf starch content between both parts. In addition, polymorphism analysis was also performed on the VAR2 gene partial sequences. Based on leaf anatomy structure, the difference between variegated zones is only in the number of chloroplasts, the diameter of parenchyma cells, and chloroplasts. The chloroplast diameter within the white part is smaller than the green part. The chloroplasts’ diameters in the green part are two times larger than the white part, with four times more in amount. The two parts were formed due to differences in the distribution pattern of functional chloroplasts in the leaves. The concentration of chlorophyll in the green part is higher than in the white part, a characteristic of the chlorophyll-type variegation. The green parts have more starch grains than the white parts. Stomata distance between variegated zones on the abaxial side is different, and it was suggested as a distinctive character between variegated zones. A thymine insertion in exon 1 contributed to nonsense and missense mutations on the VAR2 gene isolated from the white part. This mutation caused the amino acid motif to change from KAKFQ to KA—Q. This mutation is suggested to cause the variegated leaf phenomenon in P. ‘Sogo Vivien’.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
期刊最新文献
Alternating red-blue light alleviated physiological injury by reducing oxidative stress under both high light and continuous light from red-blue LEDs Comparative chloroplast genomic analysis of an important horticultural plant, Dendrobium sulcatum (Orchidaceae) and phylogenetic position in Dendrobium Triple-color FISH chromosome constitution and genome size analyses confirmed the ploidy of newly developed ten Hibiscus Sp. Cultivars with different breeding history The potential of coated iron nanoparticles for modulating of negative effects of salinity stress in Ajowan Impact of pre-harvest application of 1-methylcyclopropene (1-MCP) on fruit quality, physiological disorders and respiration rate of ‘Scilate’ apple
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1