流动会影响血浆凝块中纤维蛋白网络的结构和机械特性。

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-01-29 DOI:10.1007/s10856-024-06775-1
Hande Eyisoylu, Emma D Hazekamp, Janneke Cruts, Gijsje H Koenderink, Moniek P M de Maat
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引用次数: 0

摘要

纤维蛋白网络是血栓的主要组成部分之一。已知纤维蛋白网络特性的改变会影响血栓疾病的发生和发展,至少部分原因是纤维蛋白的机械稳定性受到了影响。大多数调查纤维蛋白在血栓特性中作用的研究都是在静态条件下制备血栓,而忽略了体内血流的影响。本研究在钱德勒回路中制备了存在和不存在血流的血浆凝块。健康捐献者的再硝化血浆分别以 0 和 30 RPM 的转速旋转。使用扫描电子显微镜和共聚焦显微镜对血凝块结构进行了表征,并将其与无约束压缩试验测得的硬度进行了关联。我们对低应变值和高应变值下的纤维蛋白纤维密度、孔径、纤维厚度和体积刚度进行了量化。与无流动情况下形成的血块相比,流动情况下形成的血块纤维蛋白纤维更细、孔径更小、纤维蛋白网络更致密、刚度值更高。我们的研究结果表明,在开发用于研究血栓切除术效果或栓塞风险的生理学相关体外血栓模型时,流体流动是一个必须考虑的因素。
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Flow affects the structural and mechanical properties of the fibrin network in plasma clots.

The fibrin network is one of the main components of thrombi. Altered fibrin network properties are known to influence the development and progression of thrombotic disorders, at least partly through effects on the mechanical stability of fibrin. Most studies investigating the role of fibrin in thrombus properties prepare clots under static conditions, missing the influence of blood flow which is present in vivo. In this study, plasma clots in the presence and absence of flow were prepared inside a Chandler loop. Recitrated plasma from healthy donors were spun at 0 and 30 RPM. The clot structure was characterized using scanning electron microscopy and confocal microscopy and correlated with the stiffness measured by unconfined compression testing. We quantified fibrin fiber density, pore size, and fiber thickness and bulk stiffness at low and high strain values. Clots formed under flow had thinner fibrin fibers, smaller pores, and a denser fibrin network with higher stiffness values compared to clots formed in absence of flow. Our findings indicate that fluid flow is an essential factor to consider when developing physiologically relevant in vitro thrombus models used in researching thrombectomy outcomes or risk of embolization.

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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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