Sri Cindhuri Katamreddy, Bommineni Pradeep Reddy, Polavarapu B Kavi Kishor, Are Ashok Kumar, Palakolanu Sudhakar Reddy
{"title":"高粱无性组织中 Dhurrin 生物合成途径基因的鉴定和表达谱图","authors":"Sri Cindhuri Katamreddy, Bommineni Pradeep Reddy, Polavarapu B Kavi Kishor, Are Ashok Kumar, Palakolanu Sudhakar Reddy","doi":"10.1007/s11816-024-00886-5","DOIUrl":null,"url":null,"abstract":"<p>Sorghum is considered a fifth major cereal, widely used as a multipurpose crop worldwide. The use of sorghum as a major forage crop is limited due to cyanogenic glycoside dhurrin in the vegetative shoot tissues. This cyanogenic glycoside is harmful to livestock when fed as fodder. The present study selected three sorghum genotypes for estimating hydrogen cyanide potential (HCNp) in vegetative tissues under well-watered (WW) conditions. The HCNp concentration varied from genotype to genotype and ranged from 364 to 512 ppm. The HCNp estimation was observed more in ICSR 14001 with 511 ppm, followed by ICSV 93046 (443 ppm) and CSH 24 MF (364 ppm). A significant difference was noticed between the genotypes. Sequence information of dhurrin biosynthesis pathway genes was retrieved and characterized using different bioinformatic tools. The gene expression analysis of dhurrin biosynthesis pathway genes showed different expression patterns, with the highest in ICSV 93046 and less in ICSR 14001 and CSH 24 MF. Genes <i>CYP79A1</i>, <i>CYP71E1</i> and <i>UGT85B1</i> showed a 2.5- to 4 fold increase in ICSV 93046 and no significant expression in ICSR 14001 and CSH 24 MF. The genotype CSH 24 MF observed a 1.5-fold increase in <i>CYP79A1</i> gene expression, and the other genes observed no significant increase. This study assisted in identifying the contrasting genotypes inducing HCNp and the key genes of the dhurrin pathway producing hydrogen cyanide (HCN) under WW conditions, which can be used as potential candidates for gene editing, providing safe feed for the livestock.</p>","PeriodicalId":20216,"journal":{"name":"Plant Biotechnology Reports","volume":"197 1","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and expression profile of dhurrin biosynthesis pathway genes in sorghum vegetative tissues\",\"authors\":\"Sri Cindhuri Katamreddy, Bommineni Pradeep Reddy, Polavarapu B Kavi Kishor, Are Ashok Kumar, Palakolanu Sudhakar Reddy\",\"doi\":\"10.1007/s11816-024-00886-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Sorghum is considered a fifth major cereal, widely used as a multipurpose crop worldwide. The use of sorghum as a major forage crop is limited due to cyanogenic glycoside dhurrin in the vegetative shoot tissues. This cyanogenic glycoside is harmful to livestock when fed as fodder. The present study selected three sorghum genotypes for estimating hydrogen cyanide potential (HCNp) in vegetative tissues under well-watered (WW) conditions. The HCNp concentration varied from genotype to genotype and ranged from 364 to 512 ppm. The HCNp estimation was observed more in ICSR 14001 with 511 ppm, followed by ICSV 93046 (443 ppm) and CSH 24 MF (364 ppm). A significant difference was noticed between the genotypes. Sequence information of dhurrin biosynthesis pathway genes was retrieved and characterized using different bioinformatic tools. The gene expression analysis of dhurrin biosynthesis pathway genes showed different expression patterns, with the highest in ICSV 93046 and less in ICSR 14001 and CSH 24 MF. Genes <i>CYP79A1</i>, <i>CYP71E1</i> and <i>UGT85B1</i> showed a 2.5- to 4 fold increase in ICSV 93046 and no significant expression in ICSR 14001 and CSH 24 MF. The genotype CSH 24 MF observed a 1.5-fold increase in <i>CYP79A1</i> gene expression, and the other genes observed no significant increase. This study assisted in identifying the contrasting genotypes inducing HCNp and the key genes of the dhurrin pathway producing hydrogen cyanide (HCN) under WW conditions, which can be used as potential candidates for gene editing, providing safe feed for the livestock.</p>\",\"PeriodicalId\":20216,\"journal\":{\"name\":\"Plant Biotechnology Reports\",\"volume\":\"197 1\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-01-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Biotechnology Reports\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s11816-024-00886-5\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology Reports","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11816-024-00886-5","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Identification and expression profile of dhurrin biosynthesis pathway genes in sorghum vegetative tissues
Sorghum is considered a fifth major cereal, widely used as a multipurpose crop worldwide. The use of sorghum as a major forage crop is limited due to cyanogenic glycoside dhurrin in the vegetative shoot tissues. This cyanogenic glycoside is harmful to livestock when fed as fodder. The present study selected three sorghum genotypes for estimating hydrogen cyanide potential (HCNp) in vegetative tissues under well-watered (WW) conditions. The HCNp concentration varied from genotype to genotype and ranged from 364 to 512 ppm. The HCNp estimation was observed more in ICSR 14001 with 511 ppm, followed by ICSV 93046 (443 ppm) and CSH 24 MF (364 ppm). A significant difference was noticed between the genotypes. Sequence information of dhurrin biosynthesis pathway genes was retrieved and characterized using different bioinformatic tools. The gene expression analysis of dhurrin biosynthesis pathway genes showed different expression patterns, with the highest in ICSV 93046 and less in ICSR 14001 and CSH 24 MF. Genes CYP79A1, CYP71E1 and UGT85B1 showed a 2.5- to 4 fold increase in ICSV 93046 and no significant expression in ICSR 14001 and CSH 24 MF. The genotype CSH 24 MF observed a 1.5-fold increase in CYP79A1 gene expression, and the other genes observed no significant increase. This study assisted in identifying the contrasting genotypes inducing HCNp and the key genes of the dhurrin pathway producing hydrogen cyanide (HCN) under WW conditions, which can be used as potential candidates for gene editing, providing safe feed for the livestock.
期刊介绍:
Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.