调查遗传变异 CXCR1 (rs2234671) 对尿路感染患者的影响。

Q3 Medicine Human Antibodies Pub Date : 2024-01-01 DOI:10.3233/HAB-230019

Hassan Hachim Naser, Mohanad Jawad Kadhim, Hazem Almhanna

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引用次数: 0

摘要

背景：目前，尿路感染（UTI）影响着数百万人的健康，已成为一个世界性的健康问题。CXCR1-白细胞介素-8受体的遗传变异rs2234671与UTI风险的增加密切相关：本文研究了 CXCR1（rs2234671）对 UTI 患者的影响：方法：全面调查了 30 名复发性 UTI 患者和 20 名对照组的人口统计学特征。方法：对 30 名复发性 UTI 患者和 20 名对照组的人口特征进行了全面调查，并通过文化和生化方法对细菌进行了分离和鉴定。对患者和健康人的所有样本进行 DNA 提取、纯化，并使用 T-ARMS-PCR 对 IL-8 rs2234671 (C/G) SNP 进行基因分型。通过统计分析评估了结果的意义：患者的平均年龄为（34.63 ± 11.44）岁，对照组的平均年龄为（30.30 ± 8.59）岁（P= 0.156）。无明显性别差异（P= 0.804）。大肠埃希菌（63.3%）居多，其次是奇异变形杆菌（26.7%）、粪肠球菌（23.3%）、肺炎克雷伯菌（10.0%）和铜绿假单胞菌（20.0%）。细菌种类频率、年龄或性别之间没有发现明显的关联。从 CXCR1 (rs2234671) 频率比较中发现，UTI 患者的 GG 基因型发生率高于对照组（26.7% 对 15.0%），但无统计学意义。风险分析表明，GG 同源基因型和 C/G 杂合基因型不是 UTI 风险因素（OR = 2.47 和 OR = 1.85）。此外，患者和对照组的等位基因频率没有明显差异（G 等位基因：66.7% 对 66.7%；C 等位基因：33.3% 对 33.3%）：主要结论：尽管未发现 CXCR1 (rs2234671) 与UTI 之间存在明显关联，但 GG 基因型可能会增加UTI 风险的概率。需要进行更多的研究来证实和扩展这些结论。

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Investigating the impact of the genetic variant CXCR1 (rs2234671) in individuals with urinary tract infections.

Background: Urinary tract infections (UTIs) are currently posing a worldwide health concern by affecting millions of people. The genetic variant rs2234671 in the CXCR1-interleukin-8 receptor is closely related to a raised UTI risk.

Objectives: In this work, the impact of CXCR1 (rs2234671) on UTI individuals was examined.

Methods: The demographic features of 30 recurrent UTI patients and 20 controls were thoroughly investigated. Bacterial isolation and identification were performed by the implementation of cultural and biochemical methods. DNA extraction, purification of all samples from both patients and healthy people, and IL-8 rs2234671 (C/G) SNP genotyping using T-ARMS-PCR were performed. The significance of the results was evaluated by carrying out a statistical analysis.

Findings: The patient's average age was 34.63 ± 11.44 years, and controls averaged 30.30 ± 8.59 years (P= 0.156). No significant gender difference existed (P= 0.804). Escherichia coli (63.3%) was predominant, followed by Proteus mirabilis (26.7%), Enterococcus faecalis (23.3%), Klebsiella pneumoniae (10.0%), and Pseudomonas aeruginosa (20.0%). No significant association was found between bacterial species frequency, age, or sex. From the CXCR1 (rs2234671) frequency comparison, a higher GG genotype incidence in UTI patients than controls was extracted (26.7% vs. 15.0%), though not statistically significant. Risk analysis revealed that GG homozygous and C/G heterozygous genotypes were not UTI risk factors (OR = 2.47 and OR = 1.85, respectively). Moreover, the allele frequencies displayed no significant difference between the patients and controls (G allele: 66.7% vs. 66.7%; C allele: 33.3% vs. 33.3%).

Main conclusions: Although no significant association between CXCR1 (rs2234671) and UTI was found, the GG genotype may point to the increasing probability of UTI risk. Additional research is required to confirm and expand these conclusions.

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.