动物蛋白水解物可减少内脏脂肪,抑制老年小鼠的胰岛素抵抗和肝脏脂肪变性。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS Nutrition Research and Practice Pub Date : 2024-02-01 Epub Date: 2023-12-11 DOI:10.4162/nrp.2024.18.1.46
Su-Kyung Shin, Ji-Yoon Lee, Heekyong R Bae, Hae-Jin Park, Eun-Young Kwon
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引用次数: 0

摘要

背景/目的:由于与年龄有关的代谢性疾病的发病率不断上升,社会预期寿命的延长给医疗系统带来了沉重的负担。本研究使用老年小鼠比较了动物蛋白水解物(APH)和酪蛋白对代谢疾病的影响:将 8 周龄和 50 周龄的 C57BL/6J 小鼠分别作为非老年对照组(YC 组)和老年对照组(NC 组)。将老龄小鼠随机分为 3 组(NC 组、低APH[LP]组和高APH[HP]组),每种实验饮食喂养 12 周。在低APH组和高APH组中,AIN-93G食物中的酪蛋白分别被16千卡%和24千卡%的APH取代。在小鼠63周龄时将其处死,分析血浆和肝脏脂质、白色脂肪组织重量、肝脏葡萄糖、脂质和抗氧化酶活性、免疫组化染色以及与肝脏和肌肉葡萄糖代谢相关的mRNA表达:结果:与阴性对照(NC)组相比,给衰老小鼠补充 APH 能显著减少内脏脂肪(附睾脂肪、肾周脂肪、腹膜后脂肪和肠系膜脂肪)。腹腔葡萄糖耐量试验和曲线下面积分析表明,NC 组存在胰岛素抵抗,而补充 APH 可缓解这种抵抗。补充 APH 可减少肝脏葡萄糖生成,增加肝脏和肌肉对葡萄糖的利用。此外,补充 APH 还能降低肝脏脂肪酸和磷脂酸磷酸酶的活性,同时提高肝脏肉碱棕榈酰基转移酶的活性,从而改善肝脏脂肪变性。此外,在补充 APH 的组别中,红细胞硫代巴比妥酸活性物质和肝脏 H2O2 水平降低,红细胞谷胱甘肽、肝脏过氧化氢酶和谷胱甘肽过氧化物酶活性升高:结论:补充 APH 可减少老年小鼠的内脏脂肪堆积,缓解与肥胖相关的代谢疾病,包括胰岛素抵抗和肝脏脂肪变性。因此,降低分子量并提高蛋白质消化率-校正氨基酸评分的优质动物蛋白 APH 有潜力成为促进健康老龄化的膳食补充剂。
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Animal protein hydrolysate reduces visceral fat and inhibits insulin resistance and hepatic steatosis in aged mice.

Background/objectives: An increasing life expectancy in society has burdened healthcare systems substantially because of the rising prevalence of age-related metabolic diseases. This study compared the effects of animal protein hydrolysate (APH) and casein on metabolic diseases using aged mice.

Materials/methods: Eight-week-old and 50-week-old C57BL/6J mice were used as the non-aged (YC group) and aged controls (NC group), respectively. The aged mice were divided randomly into 3 groups (NC, low-APH [LP], and high-APH [HP] and fed each experimental diet for 12 weeks. In the LP and HP groups, casein in the AIN-93G diet was substituted with 16 kcal% and 24 kcal% APH, respectively. The mice were sacrificed when they were 63-week-old, and plasma and hepatic lipid, white adipose tissue weight, hepatic glucose, lipid, and antioxidant enzyme activities, immunohistochemistry staining, and mRNA expression related to the glucose metabolism on liver and muscle were analyzed.

Results: Supplementation of APH in aging mice resulted in a significant decrease in visceral fat (epididymal, perirenal, retroperitoneal, and mesenteric fat) compared to the negative control (NC) group. The intraperitoneal glucose tolerance test and area under the curve analysis revealed insulin resistance in the NC group, which was alleviated by APH supplementation. APH supplementation reduced hepatic gluconeogenesis and increased glucose utilization in the liver and muscle. Furthermore, APH supplementation improved hepatic steatosis by reducing the hepatic fatty acid and phosphatidate phosphatase activity while increasing the hepatic carnitine palmitoyltransferase activity. Furthermore, in the APH supplementation groups, the red blood cell (RBC) thiobarbituric acid reactive substances and hepatic H2O2 levels decreased, and the RBC glutathione, hepatic catalase, and glutathione peroxidase activities increased.

Conclusions: APH supplementation reduced visceral fat accumulation and alleviated obesity-related metabolic diseases, including insulin resistance and hepatic steatosis, in aged mice. Therefore, high-quality animal protein APH that reduces the molecular weight and enhances the protein digestibility-corrected amino acid score has potential as a dietary supplement for healthy aging.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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