M. A. Slyusarenko, N. P. Yevlampieva, O. S. Vezo, A. V. Malek
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On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles
Abstract
It is shown that the two-phase polymer system “polyethylene glycol–dextran” can be used with high efficiency for isolating nanoscale vesicles from human blood plasma, and a corresponding methodology is developed. The optimal parameters of the system for this purpose are determined: molecular weight of polymers, their weight ratio, operating temperature. The mechanism of interaction between the components of the polymer system and plasma components is also studied. It is established that the release of vesicles in a given size range is due to the choice of the composition of the polymer system in the region below its binodal and compliance with a certain quantitative ratio of the hydrodynamic volumes of dextran and polyethylene-glycol molecules in the plasma. The proposed method is an inexpensive and convenient way to concentrate vesicles for their subsequent biochemical analysis.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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