桉树提取物及其对迪亚拉省尿路感染致病菌的协同作用

D. A. Kadhum, E. Hamad, Shahrazad A. Khalaf, E. A. Muhsin
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引用次数: 0

摘要

评估 E. camaldulensis 提取物对从巴古拜市尿毒症患者尿样中分离出的细菌的影响。此外,还要对这些病原体进行细菌学检查。测试了分离菌对(13 种)抗生素的敏感性,细菌分离菌对这些抗生素的耐药率存在差异。结果表明,抗生素亚胺培南对研究中的细菌分离物影响最大,而细菌分离物对其他抗生素的耐药性较高。对 3 种抗生素(即庆大霉素、阿米卡星和阿莫西林)进行了最小抑菌浓度(MIC)测定。这些抗生素的最小抑菌浓度值分别介于<4-1024、<4-1024 <<512-1024) mcg/ml之间。桉树乙醇提取物对变形杆菌的抗菌效果最高(o.62 克/1.5 毫升和 0.31 克/750 毫升)。对变形杆菌和假单胞菌的抗菌效果分别较低,但对克雷伯氏菌没有抗菌效果。选择细菌分离物的依据是它们最常见的尿路感染及其对抗生素的耐药性(肺炎金葡菌、铜绿假单胞菌和金黄色葡萄球菌)。通过将尤加利与三种抗生素(即阿米卡星和庆大霉素阿莫西林)混合,确定了混合效应的量。比较了所研究的分离菌在混合抗生素前后的最低抑菌浓度。混合抗生素对所有细菌分离物都有协同作用。另一方面,桉树和 β-内酰胺类(以及阿莫西林)对革兰氏阳性金黄色葡萄球菌分离物的协同作用非常明显。
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Eucalyptus extract and its synergistic effect on bacteria causing urinary tract infections in Diyala province
To assess the impact of E. camaldulensis extract against bacteria that are isolated from urine sample from UTI patient in Baquba city Then mix this plant with antibiotics to see its effect. In addition to examine those pathogens bacteriologically. The sensitivity of the isolates to (13) antibiotics was tested, and the bacterial isolates showed a variation in the rates of their resistance to these antibiotics. The results showed that the antibiotic Imipenem had the most effect on the bacterial isolates under study, while the bacterial isolates showed high resistance to the rest of the antibiotics. The minimum inhibitory concentration (MIC) was determined for 3 antibiotics, namely, Gentamycin, Amikacin and Amoxicillin. These values for the antibiotics ranged between <4-1024, <4-1024 <<512-1024) mcg/ml, respectively. The Ethanolic Extract of Eucalyptus Exhibit highest antibacterial effect on S.areus on (o.62 g/1.5 ml and 0.31g/750ml). Lower respectively antibacterial effect on Proteus and Pseudomonas but no antibacterial effect against Klebsiella. Bacterial isolates were selected on the basis of their most frequent urinary tract infections and their resistance to the effect of antibiotics (K. pneumoniae, P. aeruginosa and S. aureus). The amount of the mixed effect was determined by mixing Eucalyptus with three antibiotics, namely Amikacin and Gentamycin Amoxicillin. The minimum inhibitory concentration of the isolates under study was compared before and after mixing the antibiotics. The effect of mixing was a synergistic effect for all bacterial isolates. On the other hand, the synergistic effect was clear on the gram-positive isolates of S. aureus between eucalyptus and beta-lactams (and Amoxicillin).
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