利用超细纤维为胶原组织制作仿生移植物。

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2024-01-01 DOI:10.3233/BME-230193
Fariza Mukasheva, Ainur Zhanbassynova, Cevat Erisken
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引用次数: 0

摘要

背景:韧带是连接骨与骨的软组织,在严重损伤或断裂的情况下,韧带无法自我愈合,这主要是因为韧带血管不发达且具有动态性质。组织工程学可利用模仿原生韧带结构的支架来恢复受伤组织的功能。前交叉韧带(ACL)中的胶原纤维直径从 20 纳米到 300 纳米不等,这决定了组织的物理和机械性能。此外,前交叉韧带组织中的胶原纤维呈双峰分布。目前,制造复制这种结构的支架是一项重大挑战:这项工作旨在 i) 测量牛前交叉韧带组织胶原蛋白的直径;ii) 研究 100 纳米以下纤维的制造方法;iii) 制造具有双峰直径分布(两个峰值)的对齐支架,使其与健康的前交叉韧带结构相似。假设可以通过电纺丝聚己内酯(PCL)溶液制造这种支架:为了验证这一假设,我们用丙酮和甲酸以及吡啶配制了各种 PCL 溶液,并通过电纺丝生成了 100 纳米以下的纤维。然后,对配方进行调整,生成直径在 100 纳米到 200 纳米之间的纳米纤维。最后,将这些溶液结合到共电纺工艺(即双喷丝板电纺)中,制造出具有双峰分布的仿生物支架:结果:分别对 8%和 15%的 PCL 溶液进行电纺丝后,产生了直径小于和大于 100 纳米的纤维。组合支架呈现出排列整齐的纤维的双峰分布,峰值在 80 纳米和 180 纳米左右,从而模拟了健康 ACL 组织的胶原纤维:这项研究旨在改善广大患者的健康状况,提高他们的生活质量,因此有望产生广泛的社会影响。
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Biomimetic grafts from ultrafine fibers for collagenous tissues.

Background: The ligament is the soft tissue that connects bone to bone and, in case of severe injury or rupture, it cannot heal itself mainly because of its poor vascularity and dynamic nature. Tissue engineering carries the potential to restore the injured tissue functions by utilization of scaffolds mimicking the structure of native ligament. Collagen fibrils in the anterior cruciate ligament (ACL) have a diameter ranging from 20 to 300 nm, which defines the physical and mechanical properties of the tissue. Also, the ACL tissue exhibited a bimodal distribution of collagen fibrils. Currently, the ability to fabricate scaffolds replicating this structure is a significant challenge.

Objective: This work aims at i) measuring the diameter of collagens of bovine ACL tissue, ii) investigating the fabrication of sub-100 nm fibers, and iii) fabricating aligned scaffolds with bimodal diameter distribution (with two peaks) resembling the healthy ACL structure. It is hypothesized that such scaffolds can be produced by electrospinning polycaprolactone (PCL) solutions.

Methods: To test the hypothesis, various PCL solutions were formulated in acetone and formic acid in combination with pyridine, and electrospun to generate sub-100 nm fibers. Next, this formulation was adjusted to produce nanofibers with a diameter between 100 nm and 200 nm. Finally, these solutions were combined in the co-electrospinning process, i.e., two-spinneret electrospinning, to fabricate biomimetic scaffolds with a bimodal distribution.

Results: Electrospinning of 8% and 15% PCL solutions, respectively, resulted in the production of fibers with diameters below and above 100 nm. The combined scaffold exhibited a bimodal distribution of aligned fibers with peaks around 80 and 180 nm, thus mimicking the collagen fibrils of healthy ACL tissue.

Conclusion: This research is expected to have a society-wide impact because it aims to enhance the health condition and life quality of a wide range of patients.

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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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