Yusuke Tatsumi, Harumi Kawaki, Kohei Shintani, Kyohei Ueno, Masato Hotta, Nobuo Kondoh, Michael F Burrow, Toru Nikaido
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引用次数: 0
摘要
表面预反应玻璃-离子体（S-PRG）填料是一种生物活性玻璃填料，能够释放各种离子。据报道，在培养基中加入富含硼的 S-PRG 填充剂洗脱液，可提高人牙髓源性干细胞（hDPSC）的碱性磷酸酶（ALP）活性。为了明确从 S-PRG 填充剂中洗脱出来的硼的作用，我们使用阴离子交换材料制备了不同硼浓度的改性 S-PRG 填充剂洗脱液。因此，对阴离子交换材料的元素图谱分析、吸附率、hDPSCs 增殖和 ALP 活性进行了评估。统计分析采用 Kruskal-Wallis 检验，统计显著性以 p
Bioactivity of human dental pulp-derived stem cells with boron-controlled S-PRG filler eluate by anion exchange.
Surface pre-reacted glass-ionomer (S-PRG) filler is a bioactive glass filler capable of releasing various ions. A culture medium to which was added an S-PRG filler eluate rich in boron was reported to enhance alkaline phosphatase (ALP) activity in human dental pulp-derived stem cells (hDPSC). To clarify the role of boron eluted from S-PRG fillers, the modified S-PRG filler eluate with different boron concentrations was prepared by using an anion exchange material. Therefore, elemental mapping analysis of anion exchange material, adsorption ratio, hDPSCs proliferation and ALP activity were evaluated. For statistical analysis, Kruskal-Wallis test was used, with statistical significance determined at p<0.05. ALP activity enhancement was not observed in hDPSC cultured in the medium that contained the S-PRG filler eluate from which boron had been removed. The result suggested the possibility that an S-PRG filler eluate with controlled boron release could be useful for the development of novel dental materials.
期刊介绍：
Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.
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