在富含有机物和铁(II)的条件下,古朴红单胞菌 TIE-1 的光营养铁(II)氧化作用

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-03-19 DOI:10.1111/1462-2920.16608
Verena Nikeleit, Markus Maisch, James M. Byrne, Caroline Harwood, Andreas Kappler, Casey Bryce
{"title":"在富含有机物和铁(II)的条件下,古朴红单胞菌 TIE-1 的光营养铁(II)氧化作用","authors":"Verena Nikeleit,&nbsp;Markus Maisch,&nbsp;James M. Byrne,&nbsp;Caroline Harwood,&nbsp;Andreas Kappler,&nbsp;Casey Bryce","doi":"10.1111/1462-2920.16608","DOIUrl":null,"url":null,"abstract":"<p><i>Rhodopseudomonas palustris</i> TIE-1 grows photoautotrophically with Fe(II) as an electron donor and photoheterotrophically with a variety of organic substrates. However, it is unclear whether <i>R. palustris</i> TIE-1 conducts Fe(II) oxidation in conditions where organic substrates and Fe(II) are available simultaneously. In addition, the effect of organic co-substrates on Fe(II) oxidation rates or the identity of Fe(III) minerals formed is unknown. We incubated <i>R. palustris</i> TIE-1 with 2 mM Fe(II), amended with 0.6 mM organic co-substrate, and in the presence/absence of CO<sub>2</sub>. We found that in the absence of CO<sub>2</sub>, only the organic co-substrates acetate, lactate and pyruvate, but not Fe(II), were consumed. When CO<sub>2</sub> was present, Fe(II) and all organic substrates were consumed. Acetate, butyrate and pyruvate were consumed before Fe(II) oxidation commenced, whereas lactate and glucose were consumed at the same time as Fe(II) oxidation proceeded. Lactate, pyruvate and glucose increased the Fe(II) oxidation rate significantly (by up to threefold in the case of lactate). <sup>57</sup>Fe Mössbauer spectroscopy revealed that short-range ordered Fe(III) oxyhydroxides were formed under all conditions. This study demonstrates phototrophic Fe(II) oxidation proceeds even in the presence of organic compounds, and that the simultaneous oxidation of organic substrates can stimulate Fe(II) oxidation.</p>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":"26 3","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16608","citationCount":"0","resultStr":"{\"title\":\"Phototrophic Fe(II) oxidation by Rhodopseudomonas palustris TIE-1 in organic and Fe(II)-rich conditions\",\"authors\":\"Verena Nikeleit,&nbsp;Markus Maisch,&nbsp;James M. Byrne,&nbsp;Caroline Harwood,&nbsp;Andreas Kappler,&nbsp;Casey Bryce\",\"doi\":\"10.1111/1462-2920.16608\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Rhodopseudomonas palustris</i> TIE-1 grows photoautotrophically with Fe(II) as an electron donor and photoheterotrophically with a variety of organic substrates. However, it is unclear whether <i>R. palustris</i> TIE-1 conducts Fe(II) oxidation in conditions where organic substrates and Fe(II) are available simultaneously. In addition, the effect of organic co-substrates on Fe(II) oxidation rates or the identity of Fe(III) minerals formed is unknown. We incubated <i>R. palustris</i> TIE-1 with 2 mM Fe(II), amended with 0.6 mM organic co-substrate, and in the presence/absence of CO<sub>2</sub>. We found that in the absence of CO<sub>2</sub>, only the organic co-substrates acetate, lactate and pyruvate, but not Fe(II), were consumed. When CO<sub>2</sub> was present, Fe(II) and all organic substrates were consumed. Acetate, butyrate and pyruvate were consumed before Fe(II) oxidation commenced, whereas lactate and glucose were consumed at the same time as Fe(II) oxidation proceeded. Lactate, pyruvate and glucose increased the Fe(II) oxidation rate significantly (by up to threefold in the case of lactate). <sup>57</sup>Fe Mössbauer spectroscopy revealed that short-range ordered Fe(III) oxyhydroxides were formed under all conditions. This study demonstrates phototrophic Fe(II) oxidation proceeds even in the presence of organic compounds, and that the simultaneous oxidation of organic substrates can stimulate Fe(II) oxidation.</p>\",\"PeriodicalId\":11898,\"journal\":{\"name\":\"Environmental microbiology\",\"volume\":\"26 3\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-03-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16608\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.16608\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.16608","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

棕榈假单胞菌 TIE-1 以铁(II)为电子供体进行光自养生长,并以多种有机底物进行光异养生长。然而,目前还不清楚 R. palustris TIE-1 是否能在有机底物和铁(II)同时存在的条件下进行铁(II)氧化。此外,有机辅助底物对铁(II)氧化率的影响或形成的铁(III)矿物质的特性也不得而知。我们用 2 mM Fe(II)、0.6 mM 有机辅助底物和二氧化碳存在/不存在的情况下培养 R. palustris TIE-1。我们发现,在没有二氧化碳的情况下,只有有机辅底物乙酸盐、乳酸盐和丙酮酸盐被消耗,而 Fe(II) 却没有被消耗。当二氧化碳存在时,Fe(II)和所有有机底物都被消耗。乙酸盐、丁酸盐和丙酮酸盐在 Fe(II) 氧化开始之前被消耗,而乳酸盐和葡萄糖在 Fe(II) 氧化开始的同时被消耗。乳酸、丙酮酸和葡萄糖显著提高了铁(II)的氧化速率(乳酸的氧化速率最高可达三倍)。57Fe 莫斯鲍尔光谱显示,在所有条件下都形成了短程有序的铁(III)氧氢氧化物。这项研究表明,即使存在有机化合物,光养铁(II)氧化也能进行,而且有机底物的同时氧化也能刺激铁(II)氧化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Phototrophic Fe(II) oxidation by Rhodopseudomonas palustris TIE-1 in organic and Fe(II)-rich conditions

Rhodopseudomonas palustris TIE-1 grows photoautotrophically with Fe(II) as an electron donor and photoheterotrophically with a variety of organic substrates. However, it is unclear whether R. palustris TIE-1 conducts Fe(II) oxidation in conditions where organic substrates and Fe(II) are available simultaneously. In addition, the effect of organic co-substrates on Fe(II) oxidation rates or the identity of Fe(III) minerals formed is unknown. We incubated R. palustris TIE-1 with 2 mM Fe(II), amended with 0.6 mM organic co-substrate, and in the presence/absence of CO2. We found that in the absence of CO2, only the organic co-substrates acetate, lactate and pyruvate, but not Fe(II), were consumed. When CO2 was present, Fe(II) and all organic substrates were consumed. Acetate, butyrate and pyruvate were consumed before Fe(II) oxidation commenced, whereas lactate and glucose were consumed at the same time as Fe(II) oxidation proceeded. Lactate, pyruvate and glucose increased the Fe(II) oxidation rate significantly (by up to threefold in the case of lactate). 57Fe Mössbauer spectroscopy revealed that short-range ordered Fe(III) oxyhydroxides were formed under all conditions. This study demonstrates phototrophic Fe(II) oxidation proceeds even in the presence of organic compounds, and that the simultaneous oxidation of organic substrates can stimulate Fe(II) oxidation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
期刊最新文献
Another tool in the toolbox: Aphid-specific Wolbachia protect against fungal pathogens Bacterial communities on giant kelp in the Magellan Strait: Geographical and intra-thallus patterns Bee microbiomes in a changing climate: Investigating the effects of temperature on solitary bee life history and health Understanding the ecological versatility of Tetracladium species in temperate forest soils Issue Information
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1