调查覆盆子果实表面和空气中 Cladosporium 的接种动态。

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-03-21 DOI:10.1111/1462-2920.16613
Lauren Helen Farwell, Matevz Papp-Rupar, Greg Deakin, Naresh Magan, Xiangming Xu
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引用次数: 0

摘要

树莓生产正受到新出现的真菌病原菌 Cladosporium 属的威胁。我们利用扩增序列和 qPCR 技术,研究了果实年龄、果实在多坑道中的位置、多坑道位置和采样日期对果实附生微生物组的影响。果实年龄是影响真菌微生物群落的最重要因素,其次是采样日期和多菌槽位置。相比之下,多隧道位置和果实年龄是影响细菌微生物组组成的重要因素,其次是采样日期。隧道内位置对真菌微生物组的影响很小,而对细菌微生物组没有影响。随着果实成熟,真菌多样性增加,细菌多样性减少。Cladosporium 是水果附生微生物群中最丰富的真菌,占所有真菌序列的近 44%。使用旋转式空气采样器研究了空气中克拉多孢子菌接种体的浓度(通过 qPCR 定量)在不同地点(多菌隧道内外)和不同时间(白天和夜间)的变化情况。定量的克拉多孢子菌 DNA 在白天明显高于夜间,在多菌槽内明显高于槽外。这项研究证明了附生在树莓果实上的微生物群落和空气中的 Cladosporium 接种体的动态性质,这将对树莓果实的病害风险产生影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Investigating the inoculum dynamics of Cladosporium on the surface of raspberry fruits and in the air

Raspberry production is under threat from the emerging fungal pathogenic genus Cladosporium. We used amplicon-sequencing, coupled with qPCR, to investigate how fruit age, fruit location within a polytunnel, polytunnel location and sampling date affected the fruit epiphytic microbiome. Fruit age was the most important factor impacting the fungal microbiome, followed by sampling date and polytunnel location. In contrast, polytunnel location and fruit age were important factors impacting the bacterial microbiome composition, followed by the sampling date. The within-tunnel location had a small significant effect on the fungal microbiome and no effect on the bacterial microbiome. As fruit ripened, fungal diversity increased and the bacterial diversity decreased. Cladosporium was the most abundant fungus of the fruit epiphytic microbiome, accounting for nearly 44% of all fungal sequences. Rotorod air samplers were used to study how the concentration of airborne Cladosporium inoculum (quantified by qPCR) varied between location (inside and outside the polytunnel) and time (daytime vs. nighttime). Quantified Cladosporium DNA was significantly higher during the day than the night and inside the polytunnel than the outside. This study demonstrated the dynamic nature of epiphytic raspberry fruit microbiomes and airborne Cladosporium inoculum within polytunnels, which will impact disease risks on raspberry fruit.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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