Douglas Castro , Toshitsugu Ishihara , Erik H. Hofmeister , Stuart Clark-Price , Diego A. Portela
{"title":"超声引导和 \"盲 \"坐骨神经注射技术:在大鼠尸体上进行比较。","authors":"Douglas Castro , Toshitsugu Ishihara , Erik H. Hofmeister , Stuart Clark-Price , Diego A. Portela","doi":"10.1016/j.vaa.2024.03.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To compare the success rate and extent of sciatic nerve staining with a bupivacaine–dye solution using two injection techniques: ‘blind’ or ultrasound-guided approach.</p></div><div><h3>Study design</h3><p>Prospective, experimental, randomized, cadaveric study.</p></div><div><h3>Animals</h3><p>Adult female Wistar rat cadavers [<em>n</em> = 24, mass 352 g (323–374)].</p></div><div><h3>Methods</h3><p>Each sciatic nerve was randomly allocated to one of two groups: ‘blind’ (group B) or ultrasound-guided approach (group US) to injection. Following injection of bupivacaine–dye solution (0.1 mL), gross anatomical dissection was performed to visualize nerve staining, categorizing it as either positive or negative. The length of nerve staining was then measured and visual inspection conducted to identify potential nerve damage. Fisher's exact test was used to compare positive or negative nerve staining, and the Wilcoxon signed rank test used to compare the length of nerve staining between groups.</p></div><div><h3>Results</h3><p>In group B, the bupivacaine–dye solution stained 16/24 sciatic nerves (67% success). In group US, staining was successfully observed in all 24 nerves (100% success, <em>p</em> < 0.004). The length of nerve staining [median (interquartile range)] was 2 (2–3) mm in group B and 5 (4–6) mm in group US (<em>p</em> < 0.001). One sciatic nerve in group B had injectate distributed over 16 mm, suggestive of an intraneural injection. No signs of laceration or nerve damage were visible under 6× magnification in either group.</p></div><div><h3>Conclusions and clinical relevance</h3><p>The ultrasound-guided approach for sciatic nerve injection demonstrated a higher success rate with superior injectate distribution when compared with the ‘blind’ approach. Ultrasound guidance is recommended over a ‘blind’ approach for sciatic nerve block in rats when possible.</p></div>","PeriodicalId":23626,"journal":{"name":"Veterinary anaesthesia and analgesia","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultrasound-guided and ‘blind’ sciatic nerve injection techniques: Comparison in rat cadavers\",\"authors\":\"Douglas Castro , Toshitsugu Ishihara , Erik H. Hofmeister , Stuart Clark-Price , Diego A. Portela\",\"doi\":\"10.1016/j.vaa.2024.03.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To compare the success rate and extent of sciatic nerve staining with a bupivacaine–dye solution using two injection techniques: ‘blind’ or ultrasound-guided approach.</p></div><div><h3>Study design</h3><p>Prospective, experimental, randomized, cadaveric study.</p></div><div><h3>Animals</h3><p>Adult female Wistar rat cadavers [<em>n</em> = 24, mass 352 g (323–374)].</p></div><div><h3>Methods</h3><p>Each sciatic nerve was randomly allocated to one of two groups: ‘blind’ (group B) or ultrasound-guided approach (group US) to injection. Following injection of bupivacaine–dye solution (0.1 mL), gross anatomical dissection was performed to visualize nerve staining, categorizing it as either positive or negative. The length of nerve staining was then measured and visual inspection conducted to identify potential nerve damage. Fisher's exact test was used to compare positive or negative nerve staining, and the Wilcoxon signed rank test used to compare the length of nerve staining between groups.</p></div><div><h3>Results</h3><p>In group B, the bupivacaine–dye solution stained 16/24 sciatic nerves (67% success). In group US, staining was successfully observed in all 24 nerves (100% success, <em>p</em> < 0.004). The length of nerve staining [median (interquartile range)] was 2 (2–3) mm in group B and 5 (4–6) mm in group US (<em>p</em> < 0.001). One sciatic nerve in group B had injectate distributed over 16 mm, suggestive of an intraneural injection. No signs of laceration or nerve damage were visible under 6× magnification in either group.</p></div><div><h3>Conclusions and clinical relevance</h3><p>The ultrasound-guided approach for sciatic nerve injection demonstrated a higher success rate with superior injectate distribution when compared with the ‘blind’ approach. Ultrasound guidance is recommended over a ‘blind’ approach for sciatic nerve block in rats when possible.</p></div>\",\"PeriodicalId\":23626,\"journal\":{\"name\":\"Veterinary anaesthesia and analgesia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary anaesthesia and analgesia\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1467298724000333\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary anaesthesia and analgesia","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1467298724000333","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Ultrasound-guided and ‘blind’ sciatic nerve injection techniques: Comparison in rat cadavers
Objective
To compare the success rate and extent of sciatic nerve staining with a bupivacaine–dye solution using two injection techniques: ‘blind’ or ultrasound-guided approach.
Adult female Wistar rat cadavers [n = 24, mass 352 g (323–374)].
Methods
Each sciatic nerve was randomly allocated to one of two groups: ‘blind’ (group B) or ultrasound-guided approach (group US) to injection. Following injection of bupivacaine–dye solution (0.1 mL), gross anatomical dissection was performed to visualize nerve staining, categorizing it as either positive or negative. The length of nerve staining was then measured and visual inspection conducted to identify potential nerve damage. Fisher's exact test was used to compare positive or negative nerve staining, and the Wilcoxon signed rank test used to compare the length of nerve staining between groups.
Results
In group B, the bupivacaine–dye solution stained 16/24 sciatic nerves (67% success). In group US, staining was successfully observed in all 24 nerves (100% success, p < 0.004). The length of nerve staining [median (interquartile range)] was 2 (2–3) mm in group B and 5 (4–6) mm in group US (p < 0.001). One sciatic nerve in group B had injectate distributed over 16 mm, suggestive of an intraneural injection. No signs of laceration or nerve damage were visible under 6× magnification in either group.
Conclusions and clinical relevance
The ultrasound-guided approach for sciatic nerve injection demonstrated a higher success rate with superior injectate distribution when compared with the ‘blind’ approach. Ultrasound guidance is recommended over a ‘blind’ approach for sciatic nerve block in rats when possible.
期刊介绍:
Veterinary Anaesthesia and Analgesia is the official journal of the Association of Veterinary Anaesthetists, the American College of Veterinary Anesthesia and Analgesia and the European College of Veterinary Anaesthesia and Analgesia. Its purpose is the publication of original, peer reviewed articles covering all branches of anaesthesia and the relief of pain in animals. Articles concerned with the following subjects related to anaesthesia and analgesia are also welcome:
the basic sciences;
pathophysiology of disease as it relates to anaesthetic management
equipment
intensive care
chemical restraint of animals including laboratory animals, wildlife and exotic animals
welfare issues associated with pain and distress
education in veterinary anaesthesia and analgesia.
Review articles, special articles, and historical notes will also be published, along with editorials, case reports in the form of letters to the editor, and book reviews. There is also an active correspondence section.