Reynoutria multiflora 近乎完整的基因组组装揭示了二苯乙烯类和蒽醌类化合物生物合成的遗传基础

IF 3.7 1区 生物学 Q1 Agricultural and Biological Sciences Journal of Systematics and Evolution Pub Date : 2024-03-28 DOI:10.1111/jse.13068
Qiang He, Yuqing Miao, Xinyuan Zheng, Yaru Wang, Yitao Wang, Zheng Jia, Hongyu Zhang, Yu Wang, Yao Xiao, Cailian Du, Wei Li, Longsheng Xing, Huilong Du
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摘要

五味子是中国广泛使用的药用植物。其药用化合物主要是二苯乙烯类和蒽醌类化合物,在抗衰老、抗炎和抗氧化方面具有重要的药理活性,但其生物合成途径在很大程度上仍未得到解决。在此,我们报告了一个接近完整的多花蔷薇基因组,该基因组由 1.39 Gb 组成,等位基因 N50 为 122.91 Mb,仅有一个缺口。基因组进化分析表明,最近两次长末端重复序列(LTRs)的爆发极大地增加了多花金花菊的基因组大小,而且在多花金花菊和鞑靼金花菊的基因组之间观察到了许多大的染色体重排。比较基因组学分析表明,最近一次蓼科植物特有的全基因组复制导致多花金银花中与耐病性以及二苯乙烯类和蒽醌类化合物的生物合成有关的基因家族显著扩大。结合转录组学和代谢组学分析,我们阐明了多花连翘根部药用成分含量在不同生长年份动态变化的分子机制。此外,我们还发现了几个负责蒽醌和二苯乙烯生物合成的假定关键基因。我们发现了一个在雄花中高表达的二苯乙烯合成酶基因 PM0G05131,它可能在多花罗汉果中二苯乙烯的积累过程中发挥了重要作用。这些基因组数据将加快药用植物中蒽醌和二苯乙烯类化合物生物合成途径的发现。
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The near-complete genome assembly of Reynoutria multiflora reveals the genetic basis of stilbenes and anthraquinones biosynthesis
Reynoutria multiflora is a widely used medicinal plant in China. Its medicinal compounds are mainly stilbenes and anthraquinones which possess important pharmacological activities in anti-aging, anti-inflammatory and anti-oxidation, but their biosynthetic pathways are still largely unresolved. Here, we reported a near-complete genome assembly of R. multiflora consisting of 1.39 Gb with a contig N50 of 122.91 Mb and only one gap left. Genome evolution analysis revealed that two recent bursts of long terminal repeats (LTRs) contributed significantly to the increased genome size of R. multiflora, and numerous large chromosome rearrangements were observed between R. multiflora and Fagopyrum tataricum genomes. Comparative genomics analysis revealed that a recent whole-genome duplication specific to Polygonaceae led to a significant expansion of gene families associated with disease tolerance and the biosynthesis of stilbenes and anthraquinones in R. multiflora. Combining transcriptomic and metabolomic analyses, we elucidated the molecular mechanisms underlying the dynamic changes in content of medicinal ingredients in R. multiflora roots across different growth years. Additionally, we identified several putative key genes responsible for anthraquinone and stilbene biosynthesis. We identified a stilbene synthase gene PM0G05131 highly expressed in roost, which may exhibit an important role in the accumulation of stilbenes in R. multiflora. These genomic data will expedite the discovery of anthraquinone and stilbenes biosynthesis pathways in medicinal plants.
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来源期刊
Journal of Systematics and Evolution
Journal of Systematics and Evolution Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
7.40
自引率
8.10%
发文量
1368
审稿时长
6-12 weeks
期刊介绍: Journal of Systematics and Evolution (JSE, since 2008; formerly Acta Phytotaxonomica Sinica) is a plant-based international journal newly dedicated to the description and understanding of the biological diversity. It covers: description of new taxa, monographic revision, phylogenetics, molecular evolution and genome evolution, evolutionary developmental biology, evolutionary ecology, population biology, conservation biology, biogeography, paleobiology, evolutionary theories, and related subjects.
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