多种毒素和一种蛋白酶增强了荧光假单胞菌 PpR24 杀死蚜虫的能力。

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-04-01 DOI:10.1111/1462-2920.16604
Deepa Paliwal, Mojgan Rabiey, Tim H. Mauchline, Keywan Hassani-Pak, Ralf Nauen, Carol Wagstaff, Simon Andrews, Chris Bass, Robert W. Jackson
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引用次数: 0

摘要

蚜虫是全球重要的害虫,对多种作物造成危害。由于杀虫剂的抗药性,迫切需要开发替代控制策略。在之前的研究中,我们发现荧光假单胞菌 PpR24 可以口服感染并杀死具有抗药性的绿桃蚜(Myzus persicae)。然而,PpR24 杀虫能力的遗传基础仍不清楚。PpR24 的基因组测序证实了各种杀虫毒素的存在,如 Tc(毒素复合物)、Rhs(重排热点)元件和其他杀虫蛋白酶。蚜虫感染 PpR24 后,RNA-Seq 分析显示有 193 个蚜虫基因发生了差异表达,其中 16 个解毒基因下调。此外,1325 个 PpR24 基因(542 个上调,783 个下调)也有差异表达,其中包括负责次生代谢物生物合成、铁限制反应、氧化应激抗性和毒力因子的基因。对编码一种分泌蛋白酶(AprX)和四种毒素成分(两种 TcA 样;一种 TcB 样;一种 TcC 样杀虫毒素)的候选毒力基因进行单缺失和双缺失后发现,这五种基因都对杀死蚜虫有显著作用,尤其是 AprX。这种全面的宿主-病原体转录组分析为了解细菌介导的蚜虫死亡的分子基础以及 PpR24 作为有效生物控制剂的潜力提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Multiple toxins and a protease contribute to the aphid-killing ability of Pseudomonas fluorescens PpR24

Aphids are globally important pests causing damage to a broad range of crops. Due to insecticide resistance, there is an urgent need to develop alternative control strategies. In our previous work, we found Pseudomonas fluorescens PpR24 can orally infect and kill the insecticide-resistant green-peach aphid (Myzus persicae). However, the genetic basis of the insecticidal capability of PpR24 remains unclear. Genome sequencing of PpR24 confirmed the presence of various insecticidal toxins such as Tc (toxin complexes), Rhs (rearrangement hotspot) elements, and other insect-killing proteases. Upon aphids infection with PpR24, RNA-Seq analysis revealed 193 aphid genes were differentially expressed with down-regulation of 16 detoxification genes. In addition, 1325 PpR24 genes (542 were upregulated and 783 downregulated) were subject to differential expression, including genes responsible for secondary metabolite biosynthesis, the iron-restriction response, oxidative stress resistance, and virulence factors. Single and double deletion of candidate virulence genes encoding a secreted protease (AprX) and four toxin components (two TcA-like; one TcB-like; one TcC-like insecticidal toxins) showed that all five genes contribute significantly to aphid killing, particularly AprX. This comprehensive host–pathogen transcriptomic analysis provides novel insight into the molecular basis of bacteria-mediated aphid mortality and the potential of PpR24 as an effective biocontrol agent.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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