拟南芥中的核 WD40 重复蛋白 PRL1 可调节 MYB4 转录因子的稳定性

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-04-01 DOI:10.1016/j.cpb.2024.100341
Mehali Mitra , Samrat Banerjee , Bhagath Kumar Palaka , Swarup Roy Choudhury , Sujit Roy
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引用次数: 0

摘要

MYB4 是 MYB 转录因子 R2R3 型亚家族的成员,在调节植物体内吸收紫外线的苯丙酮类物质的积累方面起着至关重要的作用。较长时间的紫外线-B 暴露会下调拟南芥中 MYB4 基因的表达。MYB4 蛋白通过与自身启动子结合来抑制自身的表达。然而,目前人们对 MYB4 蛋白在体内的翻译后调控几乎一无所知。在这里,我们提供了拟南芥中 MYB4 蛋白在体内磷酸化并被泛素-26S 蛋白酶体依赖途径靶向的证据。免疫沉淀、免疫印迹和磷蛋白染色实验表明,在长日照条件下的 24 小时时间跨度内,MYB4 的积累模式和磷酸化程度在光照条件下均有所增加。酵母双杂交和双分子荧光互补实验表明,MYB4 在体内与核 WD40 重复蛋白 PRL1 直接相互作用。在无蛋白酶体抑制剂和有蛋白酶体抑制剂的情况下进行的无细胞蛋白降解试验表明,MYB4 是以依赖泛素蛋白酶体的方式降解的。此外,在表达 35 S:AtMYB4 (35 S:AtMYB4-atmyb4-1 )的转基因atmyb4-1 突变株系和atprl1-1 突变株系中对 MYB4 蛋白积累水平的分析表明,PRL1 调节拟南芥中 MYB4 的稳定性。总之,我们的研究结果为拟南芥体内MYB4蛋白翻译后修饰和稳定性调控的可能机制提供了重要信息。
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A nuclear WD40 repeat protein PRL1 regulates stability of MYB4 transcription factor in Arabidopsis

MYB4, a member of the R2R3-type subfamily of MYB transcription factor plays a crucial role in regulating the accumulation of UV-B absorbing phenylpropanoids in plants. UV-B exposure for a longer duration down-regulates the expression of MYB4 gene in Arabidopsis. MYB4 protein represses its own expression by binding to its own promoter. However, at present practically nothing is known about the post-translational regulation of MYB4 protein in vivo. Here, we provide evidence that in Arabidopsis MYB4 protein is phosphorylated in vivo and is targeted by the ubiquitin-26S proteasome-dependent pathway. Immunoprecipitation, immunoblotting, and phosphoprotein staining experiments have revealed that both the accumulation pattern and phosphorylation of MYB4 increase in the light condition during the 24 hours time span under long-day conditions. Yeast two-hybrid and bimolecular fluorescence complementation assays have shown that MYB4 directly interacts with a nuclear WD40 repeat protein, PRL1 in vivo. Cell-free protein degradation assay in the absence and presence of proteasome inhibitor indicates that MYB4 is degraded in a ubiquitin proteasome-dependent manner. Furthermore, analyses of MYB4 protein accumulation levels in transgenic atmyb4–1 mutant line expressing 35S:AtMYB4 (35S:AtMYB4-atmyb4–1) and atprl1–1 mutant line indicate that PRL1 regulate stability of MYB4 in Arabidopsis. Overall, our results provide important information on the possible mechanism of post-translational modification and regulation of stability of MYB4 protein in Arabidopsis in vivo.

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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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