巴格达省屠宰场酵母菌的分离与鉴定

Hayder R. Mashari, Zainab A. A. Al-haddad
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摘要

这项研究的目的是从屠宰场环境中分离和鉴定酵母菌,包括屠宰前和屠宰后的设备标本以及屠宰场内屠宰的绵羊尸体的重要器官拭子,标本是在巴格达省冬季(2023 年)采集的;从屠宰场采集了 200 份样本,其中 50 份样本在屠宰前采集,50 份样本在屠宰后采集,最后从绵羊尸体上采集了 100 份样本,分别从肺、肝脏、心脏和脾脏中采集了 25 份拭子;结果显示,屠宰前分离到的酵母菌比例为(52%),屠宰后下降到(42%),而从羊屠体重要器官分离到的酵母菌比例为(22%),其中屠宰场环境中最明显的是白僵菌。白隐球菌(C. albicans)的分离率为(10%),其次是粘液红球菌(Rhodotorula mucilaginosa)(7.5%),然后是副丝状球菌(C. parapsilosis)的分离率为(4.5%),白隐球菌(C. Cryptococcus albidus)和吉利蒙地球菌(C. guilliermondii)的分离率为(3%);C.法马塔隐球菌的出现率为(2.5%),泽兰隐球菌(C. zeylanoides)和斯波里卡隐球菌(C. sphaerica)的出现率同样为(1%);最后,粗毛隐球菌(C. rugose)、卢西塔尼亚隐球菌(C. Lusitaniae)、热带隐球菌(C. tropicalis)和克鲁塞隐球菌(C. krusei)的出现率分别为(0.5%)。
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Isolation and Identification of Yeasts from Slaughterhouses in Baghdad province
The aim of this research was isolation and identification of yeasts from slaughterhouse environment which including  equipment specimens before slaughtered and after slaughtered as well as vital organs swabs in  sheep carcasses were slaughtered  inside of slaughterhouse ,   the specimens were collected in Baghdad province during winter season (2023);and  two hundred (200) samples were collected from abattoirs divided in  to (50) samples before slaughtered and (50) samples after slaughtered and finally another (100) samples were obtained from sheep carcasses which were distributed into twenty-five(25)swabs from each of lung,liver,heart and spleen; these samples were inoculated  on Sabouraud Dextrose Agar (SDA),so the results revealed that the percentage of yeasts isolated before slaughtered was  (52%) while after slaughtered it decreased  to  (42%),while percentage of isolation from vital organs of sheep carcasses (22%) in which most visible in abattoirs environment  C. albicans was in percent of (10%) then  Rhodotorula mucilaginosa was recorded (7.5%) , whereas  after C. parapsilosis appeared in percent of (4.5%)   while Cryptococcus  albidus and C. guilliermondii appeared in percent of(3%)  ; C. famata appeared in percent of (2.5%), both C. zeylanoides and C. sphaerica  were recorded the  same percent of (1%);finally each of C.rugose, C. Lusitaniae ,C.tropicalis, and C. krusei were appeared in percent of (0.5%).
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