Faizan Ahmed Sadiq , Koen De Reu , Nan Yang , Mette Burmølle , Marc Heyndrickx
{"title":"乳制品生物膜中的种间相互作用驱动着群落结构以及对清洁和消毒的反应","authors":"Faizan Ahmed Sadiq , Koen De Reu , Nan Yang , Mette Burmølle , Marc Heyndrickx","doi":"10.1016/j.bioflm.2024.100195","DOIUrl":null,"url":null,"abstract":"<div><p>Interspecies interactions within a biofilm community influence population dynamics and community structure, which in turn may affect the bacterial stress response to antimicrobials. This study was conducted to assess the impact of interactions between <em>Kocuria salsicia</em> and a three-species biofilm community (comprising <em>Stenotrophomonas rhizophila</em>, <em>Bacillus licheniformis</em>, and <em>Microbacterium lacticum</em>) on biofilm mass, the abundance of individual species, and their survival under a laboratory-scale cleaning and disinfection (C&D) regime. The presence of <em>K. salsicia</em> enhanced the cell numbers of all three species in pairwise interactions. The outcomes derived from summing up pairwise interactions did not accurately predict the bacterial population dynamics within communities of more than two species. In four-species biofilms, we observed the dominance of <em>S. rhizophila</em> and <em>B. licheniformis</em>, alongside a concurrent reduction in the cell counts of <em>K. salsicia</em> and <em>M. lacticum</em>. This pattern suggests that the underlying interactions are not purely non-transitive; instead, a more complex interplay results in the dominance of specific species. We observed that bacterial spatial organization and matrix production in different mixed-species combinations affected survival in response to C&D. Confocal microscopy analysis of spatial organization showed that <em>S. rhizophila</em> localized on the biofilm formed by <em>B. licheniformis</em> and <em>M. lacticum</em>, and <em>S. rhizophila</em> was more susceptible to C&D. Matrix production in <em>B. licheniformis</em>, evidenced by alterations in biofilm mass and by scanning electron microscopy, demonstrated its protective role against C&D, not only for this species itself, but also for neighbouring species. Our findings emphasise that various social interactions within a biofilm community not only affect bacterial population dynamics but also influence the biofilm community's response to C&D stress.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":null,"pages":null},"PeriodicalIF":5.9000,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590207524000200/pdfft?md5=c57f037c6f93b4332c14177591b53dfa&pid=1-s2.0-S2590207524000200-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Interspecies interactions in dairy biofilms drive community structure and response against cleaning and disinfection\",\"authors\":\"Faizan Ahmed Sadiq , Koen De Reu , Nan Yang , Mette Burmølle , Marc Heyndrickx\",\"doi\":\"10.1016/j.bioflm.2024.100195\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Interspecies interactions within a biofilm community influence population dynamics and community structure, which in turn may affect the bacterial stress response to antimicrobials. This study was conducted to assess the impact of interactions between <em>Kocuria salsicia</em> and a three-species biofilm community (comprising <em>Stenotrophomonas rhizophila</em>, <em>Bacillus licheniformis</em>, and <em>Microbacterium lacticum</em>) on biofilm mass, the abundance of individual species, and their survival under a laboratory-scale cleaning and disinfection (C&D) regime. The presence of <em>K. salsicia</em> enhanced the cell numbers of all three species in pairwise interactions. The outcomes derived from summing up pairwise interactions did not accurately predict the bacterial population dynamics within communities of more than two species. In four-species biofilms, we observed the dominance of <em>S. rhizophila</em> and <em>B. licheniformis</em>, alongside a concurrent reduction in the cell counts of <em>K. salsicia</em> and <em>M. lacticum</em>. This pattern suggests that the underlying interactions are not purely non-transitive; instead, a more complex interplay results in the dominance of specific species. We observed that bacterial spatial organization and matrix production in different mixed-species combinations affected survival in response to C&D. Confocal microscopy analysis of spatial organization showed that <em>S. rhizophila</em> localized on the biofilm formed by <em>B. licheniformis</em> and <em>M. lacticum</em>, and <em>S. rhizophila</em> was more susceptible to C&D. Matrix production in <em>B. licheniformis</em>, evidenced by alterations in biofilm mass and by scanning electron microscopy, demonstrated its protective role against C&D, not only for this species itself, but also for neighbouring species. 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引用次数: 0
摘要
生物膜群落中的种间相互作用会影响种群动态和群落结构,进而影响细菌对抗菌剂的应激反应。本研究旨在评估 Kocuria salsicia 与三物种生物膜群落(包括根茎坏死单胞菌、地衣芽孢杆菌和乳酸微杆菌)之间的相互作用对生物膜质量、单个物种的丰度以及它们在实验室规模的清洁和消毒(C&D)机制下的存活率的影响。K. salsicia 的存在提高了成对相互作用中所有三种物种的细胞数量。将成对相互作用相加得出的结果并不能准确预测两个以上物种群落中的细菌种群动态。在四种生物膜中,我们观察到 S. rhizophila 和 B. licheniformis 占主导地位,同时 K. salsicia 和 M. lacticum 的细胞数减少。这种模式表明,潜在的相互作用并不纯粹是非传递性的;相反,更复杂的相互作用导致了特定物种的优势地位。我们观察到,不同混合物种组合中的细菌空间组织和基质生产会影响对 C&D 反应的存活率。共聚焦显微镜对空间组织的分析表明,根瘤嗜血杆菌定位于地衣芽孢杆菌和乳酸杆菌形成的生物膜上,而且根瘤嗜血杆菌更容易受到 C&D 的影响。地衣芽孢杆菌产生的基质通过生物膜质量的改变和扫描电子显微镜得到证明,这表明它对 C&D 具有保护作用,不仅对该物种本身,而且对邻近物种也是如此。我们的研究结果强调,生物膜群落中的各种社会互动不仅会影响细菌的种群动态,还会影响生物膜群落对 C&D 压力的反应。
Interspecies interactions in dairy biofilms drive community structure and response against cleaning and disinfection
Interspecies interactions within a biofilm community influence population dynamics and community structure, which in turn may affect the bacterial stress response to antimicrobials. This study was conducted to assess the impact of interactions between Kocuria salsicia and a three-species biofilm community (comprising Stenotrophomonas rhizophila, Bacillus licheniformis, and Microbacterium lacticum) on biofilm mass, the abundance of individual species, and their survival under a laboratory-scale cleaning and disinfection (C&D) regime. The presence of K. salsicia enhanced the cell numbers of all three species in pairwise interactions. The outcomes derived from summing up pairwise interactions did not accurately predict the bacterial population dynamics within communities of more than two species. In four-species biofilms, we observed the dominance of S. rhizophila and B. licheniformis, alongside a concurrent reduction in the cell counts of K. salsicia and M. lacticum. This pattern suggests that the underlying interactions are not purely non-transitive; instead, a more complex interplay results in the dominance of specific species. We observed that bacterial spatial organization and matrix production in different mixed-species combinations affected survival in response to C&D. Confocal microscopy analysis of spatial organization showed that S. rhizophila localized on the biofilm formed by B. licheniformis and M. lacticum, and S. rhizophila was more susceptible to C&D. Matrix production in B. licheniformis, evidenced by alterations in biofilm mass and by scanning electron microscopy, demonstrated its protective role against C&D, not only for this species itself, but also for neighbouring species. Our findings emphasise that various social interactions within a biofilm community not only affect bacterial population dynamics but also influence the biofilm community's response to C&D stress.