Africa Smith de Diego, Oreoluwa V. Griffiths, Matthew P. Johnson, Marco de Montis, Michael Pycraft Hughes
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引用次数: 0
摘要
在许多应用中,上游样品处理需要浓缩流动中的分散颗粒;这可能是为了提高浓度(例如,提高生物传感器的精度),也可能是为了降低浓度(例如,去除流动中的污染物)。交流电动现象--介电泳(DEP)已被广泛用于流动中的颗粒捕集,但力降的幅度会随着与电极边缘的距离而迅速减小,因此只有在靠近电极表面时才能捕集到纳米级的颗粒,如病毒和细菌。这就限制了装置中的可用流速,可能导致最终装置无法用于实际应用。相反,另一种电动现象--交流电渗透(ACEO)可用于将微粒移动到电极表面,但无法从流动中捕获微粒,从而限制了样品净化或捕获-净化浓缩的能力。在本文中,我们介绍了如何优化与压力驱动流平行排列的 ACEO 电极,将其作为前驱体/预处理器,从流体中捕获颗粒,并将其集中到通道壁附近,以增强 DEP 捕获能力。结果表明,这种方法在流速高达 0.84 ml min-1 时非常有效。此外,通过模拟和共聚焦显微镜对 ACEO 设备中的三维流动结构进行的分析表明,虽然该系统具有显著的优势,但通道盖附近容积的流动结构使得虽然可以进行大量捕集,但在腔室的这一部分无法捕集颗粒,这与腔室的高度无关。
Optimization of upstream particle concentration from flow using AC electro-osmosis and dielectrophoresis
There are many applications where upstream sample processing is required to concentrate dispersed particles in flow; this may be to increase the concentration (e.g., to enhance biosensor accuracy) or to decrease it (e.g., by removing contaminants from flow). The AC electrokinetic phenomenon, dielectrophoresis (DEP), has been used widely for particle trapping for flow, but the magnitude of the force drops reduces rapidly with distance from electrode edges, so that nm-scale particles such as viruses and bacteria are only trapped when near the electrode surface. This limits the usable flow rate in the device and can render the final device unusable for practical applications. Conversely, another electrokinetic phenomenon, AC electro-osmosis (ACEO), can be used to move particles to electrode surfaces but is unable to trap them from flow, limiting their ability for sample cleanup or trap-and-purge concentration. In this paper, we describe the optimization of ACEO electrodes aligned parallel to pressure-driven flow as a precursor/preconditioner to capture particles from a flow stream and concentrate them adjacent to the channel wall to enhance DEP capture. This is shown to be effective at flow rates of up to 0.84 ml min−1. Furthermore, the analysis of the 3D flow structure in the ACEO device by both simulation and confocal microscopy suggests that while the system offers significant benefits, the flow structure in the volume near the channel lid is such that while substantial trapping can occur, particles in this part of the chamber cannot be trapped, independent of the chamber height.
期刊介绍:
Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications.
BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics.
Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary)
Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification)
Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation)
Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles)
Cell culture and analysis(single cell assays, stimuli response, stem cell transfection)
Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays)
Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers)
Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...