嗜极高氯酸盐还原菌的生物勘探:从哥伦比亚卡塔赫纳海湾沉积物中分离出的有希望的芽孢杆菌属的报告

IF 3.1 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biodegradation Pub Date : 2024-04-16 DOI:10.1007/s10532-024-10079-0
Rosa Acevedo-Barrios, Irina Tirado-Ballestas, Angela Bertel-Sevilla, Leonor Cervantes-Ceballos, Jorge L. Gallego, María Angélica Leal, David Tovar, Jesús Olivero-Verbel
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摘要

摘要 从哥伦比亚加勒比海岸沉积物中分离出三株嗜极细菌(BBCOL-009、BBCOL-014 和 BBCOL-015),它们能够在一定 pH 值(6.5 至 10.0)范围内降解高浓度高氯酸盐。形态特征包括革兰氏阴性菌株杆菌,平均大小分别为 1.75 × 0.95、2.32 × 0.65 和 3.08 × 0.70 μm。报告的菌株耐受的 pH 值范围很广(6.5 至 10.0);耐受的 NaCl 浓度(3.5 至 7.5% w/v)和 KClO4- 浓度(250 至 10000 mg/L),KClO4- 的减少量为 10%至 25%。LB 肉汤中的 NaCl(3.5%-30% w/v)和 KClO4ˉ(250-10000 mg/L)分别用于独立试验,以评估对盐度和高氯酸盐的敏感性。分离菌株在 7.5%(重量/体积)NaCl 的条件下生物量增加,在 3.5%NaCl条件下发育最佳。随后,使用含 3.5% NaCl 和 10000 mg/L ClO4ˉ 的 LB 培养基对 ClO4ˉ 的还原性进行了评估。BBCOL-009、BBCOL-014和BBCOL-015的ClO4ˉ降解率分别为10%、17%和25%。16 S rRNA基因序列将它们分别归类为柔性芽孢杆菌 T6186-2、海洋芽孢杆菌 TF-11(T)和越南芽孢杆菌 15 - 1(T),同源性为 97.5%。此外,所有分离菌株都对厄他培南、万古霉素、克拉维酸阿莫西林、青霉素和红霉素具有抗菌性,这表明它们对应激环境具有很强的适应性。从哥伦比亚卡塔赫纳湾的海洋沉积物中分离出的菌株是减少不同环境中高氯酸盐污染的合适候选菌株。尽管对高氯酸盐还原菌和抗性细菌的研究主要集中在生态和农业领域,但从天体生物学的角度来看,抗高氯酸盐细菌可以作为天体生物学研究的模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
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来源期刊
Biodegradation
Biodegradation 工程技术-生物工程与应用微生物
CiteScore
5.60
自引率
0.00%
发文量
36
审稿时长
6 months
期刊介绍: Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.
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