{"title":"罗丹明 6G@-nido-硼烷荧光聚合物的制备、持续释放和细胞成像研究","authors":"Tiantian Chai, Ying Liu, Meng Zhou, Shuo Wang, Jiankang Feng, Mengtong Zhang, Xibing Feng, Jingnan Hu, Qingxia Chu, Chichong Lu, Guofan Jin","doi":"10.1007/s13233-024-00252-y","DOIUrl":null,"url":null,"abstract":"<div><p>In this paper, rhodamine 6G and carborane were used as raw materials, and four fluorescent polymers were prepared by one-pot method, namely L100-55 fluorescent polymer (<b>L100-55-B</b>), EPO polymer (<b>EPO-B</b>), RS polymer (<b>RS</b>) and RL polymer (<b>RL</b>). The problem of poor water solubility of carborane was solved and the biocompatibility of rhodamine 6G-<i>nido</i>-carborane was improved. By simulating the release of polymers in different gastrointestinal environments, it was found that the release rate of drugs in the four coating materials was slow and controllable, with good bioavailability. Affected by the properties of the resin, L100-55 and EPO coating had the best release effect in the gastrointestinal tract. In the zeta potential test, the absolute potential values of <b>L100-55-B</b> and <b>EPO-B</b> are stable, both up to 30 mV. Transmission electron microscopy revealed that the drug was uniformly dispersed in the drug carrier material and wrapped into nanospheres with particle sizes below 500 nm. Hela cells were imaged in different acidic environments, and the results showed that the polymer had good affinity with target cells and was closely connected to target cells, indicating good biocompatibility and targeting of the polymer. This design not only solves the bioavailability problem of rhodamine 6G-<i>nido</i>-carborane, but also has a good fluorescence targeting effect.</p><h3>Graphical abstract</h3><p>Rhodamine 6G-<i>nido</i>-carborane coated by four eudragits, L100-55, EPO, RS, and RL, are released in the gastrointestinal environment. Cell imaging under a microscope shows that <b>L100-55-B</b> and <b>EPO-B</b> have a strong affinity for Hela cells. Four coating pathways, the released rhodamine 6G-<i>nido</i>-carborane targets tumor cells and exerts inhibitory effects.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div><p>\n1. The bioavailability and water solubility of carborane derivatives have been improved.</p><p>2. Rhodamine 6G is used to make the complex visible in vivo, which is convenient for monitoring the drug distribution.</p><p>3. Acrylic resin coating can change drug release performance.</p><p>4. Hela cell imaging experiments display excellent cellular permeability.</p></div>","PeriodicalId":688,"journal":{"name":"Macromolecular Research","volume":"32 6","pages":"581 - 595"},"PeriodicalIF":2.8000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation, sustained release and cell imaging studies of rhodamine 6G@-nido-carborane fluorescent polymer\",\"authors\":\"Tiantian Chai, Ying Liu, Meng Zhou, Shuo Wang, Jiankang Feng, Mengtong Zhang, Xibing Feng, Jingnan Hu, Qingxia Chu, Chichong Lu, Guofan Jin\",\"doi\":\"10.1007/s13233-024-00252-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this paper, rhodamine 6G and carborane were used as raw materials, and four fluorescent polymers were prepared by one-pot method, namely L100-55 fluorescent polymer (<b>L100-55-B</b>), EPO polymer (<b>EPO-B</b>), RS polymer (<b>RS</b>) and RL polymer (<b>RL</b>). The problem of poor water solubility of carborane was solved and the biocompatibility of rhodamine 6G-<i>nido</i>-carborane was improved. By simulating the release of polymers in different gastrointestinal environments, it was found that the release rate of drugs in the four coating materials was slow and controllable, with good bioavailability. Affected by the properties of the resin, L100-55 and EPO coating had the best release effect in the gastrointestinal tract. In the zeta potential test, the absolute potential values of <b>L100-55-B</b> and <b>EPO-B</b> are stable, both up to 30 mV. Transmission electron microscopy revealed that the drug was uniformly dispersed in the drug carrier material and wrapped into nanospheres with particle sizes below 500 nm. Hela cells were imaged in different acidic environments, and the results showed that the polymer had good affinity with target cells and was closely connected to target cells, indicating good biocompatibility and targeting of the polymer. This design not only solves the bioavailability problem of rhodamine 6G-<i>nido</i>-carborane, but also has a good fluorescence targeting effect.</p><h3>Graphical abstract</h3><p>Rhodamine 6G-<i>nido</i>-carborane coated by four eudragits, L100-55, EPO, RS, and RL, are released in the gastrointestinal environment. Cell imaging under a microscope shows that <b>L100-55-B</b> and <b>EPO-B</b> have a strong affinity for Hela cells. Four coating pathways, the released rhodamine 6G-<i>nido</i>-carborane targets tumor cells and exerts inhibitory effects.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div><p>\\n1. The bioavailability and water solubility of carborane derivatives have been improved.</p><p>2. Rhodamine 6G is used to make the complex visible in vivo, which is convenient for monitoring the drug distribution.</p><p>3. Acrylic resin coating can change drug release performance.</p><p>4. Hela cell imaging experiments display excellent cellular permeability.</p></div>\",\"PeriodicalId\":688,\"journal\":{\"name\":\"Macromolecular Research\",\"volume\":\"32 6\",\"pages\":\"581 - 595\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Macromolecular Research\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13233-024-00252-y\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Macromolecular Research","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13233-024-00252-y","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
Preparation, sustained release and cell imaging studies of rhodamine 6G@-nido-carborane fluorescent polymer
In this paper, rhodamine 6G and carborane were used as raw materials, and four fluorescent polymers were prepared by one-pot method, namely L100-55 fluorescent polymer (L100-55-B), EPO polymer (EPO-B), RS polymer (RS) and RL polymer (RL). The problem of poor water solubility of carborane was solved and the biocompatibility of rhodamine 6G-nido-carborane was improved. By simulating the release of polymers in different gastrointestinal environments, it was found that the release rate of drugs in the four coating materials was slow and controllable, with good bioavailability. Affected by the properties of the resin, L100-55 and EPO coating had the best release effect in the gastrointestinal tract. In the zeta potential test, the absolute potential values of L100-55-B and EPO-B are stable, both up to 30 mV. Transmission electron microscopy revealed that the drug was uniformly dispersed in the drug carrier material and wrapped into nanospheres with particle sizes below 500 nm. Hela cells were imaged in different acidic environments, and the results showed that the polymer had good affinity with target cells and was closely connected to target cells, indicating good biocompatibility and targeting of the polymer. This design not only solves the bioavailability problem of rhodamine 6G-nido-carborane, but also has a good fluorescence targeting effect.
Graphical abstract
Rhodamine 6G-nido-carborane coated by four eudragits, L100-55, EPO, RS, and RL, are released in the gastrointestinal environment. Cell imaging under a microscope shows that L100-55-B and EPO-B have a strong affinity for Hela cells. Four coating pathways, the released rhodamine 6G-nido-carborane targets tumor cells and exerts inhibitory effects.
1. The bioavailability and water solubility of carborane derivatives have been improved.
2. Rhodamine 6G is used to make the complex visible in vivo, which is convenient for monitoring the drug distribution.
3. Acrylic resin coating can change drug release performance.
4. Hela cell imaging experiments display excellent cellular permeability.
期刊介绍：
Original research on all aspects of polymer science, engineering and technology, including nanotechnology
Presents original research articles on all aspects of polymer science, engineering and technology
Coverage extends to such topics as nanotechnology, biotechnology and information technology
The English-language journal of the Polymer Society of Korea
Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.
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