硫嘌呤甲基转移酶(TPMT)基因表达和血清 TNF 对伊拉克溃疡性结肠炎(UC)患者依木兰反应的药物遗传学影响

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-10-01 DOI:10.61186/rbmb.12.3.438
Noor Ali, Rafid Abdulkareem
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引用次数: 0

摘要

背景:溃疡性结肠炎(UC)是一种慢性炎症性肠病(IBD),对直肠和结肠粘膜均有影响,且发病率越来越高。本研究旨在探讨硫嘌呤甲基转移酶(TPMT)基因表达和血清肿瘤坏死因子(TNF)水平对伊拉克 UC 患者对伊莫兰反应的药物遗传学影响:方法:70 名慢性 UC 患者和 30 名健康对照者参加了此次调查。采用三唑法提取 RNA,酶联免疫吸附试验(ELISA)测定 TNF。患者年龄在15-50岁之间,接受了伊莫兰治疗:患者对伊莫兰的反应各不相同,35 例患者的 TPMT 基因表达水平低于 1,导致副作用,其余 35 例患者的 TPMT 基因表达水平超过 1,表现出阳性反应。与 TNF 相关的不同程度的重度、中度和轻度 UC 患者与 Imuran 无应答有显著相关性:结论:在 UC 患者中,TPMT 基因表达与伊莫兰治疗结果之间存在明显的相关性。结论:TPMT基因表达与UC患者伊莫兰治疗结果之间存在明显的相关性,需要进一步研究以阐明其潜在机制,并将TPMT基因定位为减轻UC影响的潜在治疗靶点。
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Pharmacogenetic Effect of Thiopurine Methyl Transferase (TPMT) Gene Expression and Serum TNF on the Imuran Response in Ulcerative Colitis (UC) Iraqi Patients.

Background: Ulcerative colitis (UC), a chronic inflammatory bowel disease (IBD), exerts its impact on both rectal and colonic mucosa, with a growing incidence. This study aims to explore the pharmacogenetic influence of thiopurine methyl transferase (TPMT) gene expression and serum tumor necrosis factor (TNF) levels on the response to Imuran in Iraqi patients with UC.

Methods: Seventy individuals with chronic UC and 30 healthy controls were enrolled in this investigation. RNA extraction using the triazole method and enzyme-linked immunosorbent assay (ELISA) for TNF measurement were employed. Patients, aged 15-50 years, underwent Imuran treatment.

Results: Diverse responses to Imuran were observed among patients, with TPMT gene expression levels below 1 in 35 patients leading to side effects, while the remaining 35 patients exhibited positive responses with TPMT gene expression exceeding 1. Patients with varying degrees of severe, moderate, and mild UC associated with TNF showed a significant correlation with Imuran non-response.

Conclusions: A distinct correlation was identified between TPMT gene expression and Imuran therapy outcomes in UC patients. Further investigation is warranted to elucidate the underlying mechanism, positioning the TPMT gene as a potential therapeutic target for mitigating the impact of UC.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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