A. E. Kitova, Yu. V. Plekhanova, S. E. Tarasov, N. A. Klenova, A. N. Reshetilov
{"title":"作为细菌生物传感器受体元件的 BC/PEDOT:PSS/Graphene 导电复合材料","authors":"A. E. Kitova, Yu. V. Plekhanova, S. E. Tarasov, N. A. Klenova, A. N. Reshetilov","doi":"10.1134/s2635167623601493","DOIUrl":null,"url":null,"abstract":"<p>Тhe search for new materials with high conductivity and biocompatibility for use in biosensors is an important task. One promising material is bacterial cellulose (BC) due to its high surface area, high porosity, and biocompatibility. In this study, bacterial cellulose is modified with the PEDOT:PSS conductive gel and carbon nanomaterials to increase conductivity. Thermally expanded graphite/BC/PEDOT:PSS/graphene composition is used to immobilize <i>Gluconobacter oxydans</i> acetic-acid bacteria on the surface of a screen-printed carbon electrode. The effect of individual components of the composite on the catalytic activity of bacteria in the presence of 2,6-dichlorophenolindophenol redox mediator is studied. The addition of BC to the composition provides a higher stability of the electrode: the drop in signal within 35 days is 9%. A microbial biosensor based on TEG/PEDOT:PSS/Graphene/BC/<i>G. oxydans</i> composite show better sensitivity (36.4 µA mM<sup>–1</sup> cm<sup>–2</sup>) and lower detection limit (0.005 mM) as well as the widest linear detection range (0.005–2 mM) compared to the other compositions. Thus, bacterial cellulose modified with conductive additives can be applied as a matrix for the immobilization of bacteria in microbial biosensors and microbial fuel cells.</p>","PeriodicalId":716,"journal":{"name":"Nanotechnologies in Russia","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"BC/PEDOT:PSS/Graphene Conductive Composite As the Receptor Element for a Bacterial Biosensor\",\"authors\":\"A. E. Kitova, Yu. V. Plekhanova, S. E. Tarasov, N. A. Klenova, A. N. Reshetilov\",\"doi\":\"10.1134/s2635167623601493\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Тhe search for new materials with high conductivity and biocompatibility for use in biosensors is an important task. One promising material is bacterial cellulose (BC) due to its high surface area, high porosity, and biocompatibility. In this study, bacterial cellulose is modified with the PEDOT:PSS conductive gel and carbon nanomaterials to increase conductivity. Thermally expanded graphite/BC/PEDOT:PSS/graphene composition is used to immobilize <i>Gluconobacter oxydans</i> acetic-acid bacteria on the surface of a screen-printed carbon electrode. The effect of individual components of the composite on the catalytic activity of bacteria in the presence of 2,6-dichlorophenolindophenol redox mediator is studied. The addition of BC to the composition provides a higher stability of the electrode: the drop in signal within 35 days is 9%. A microbial biosensor based on TEG/PEDOT:PSS/Graphene/BC/<i>G. oxydans</i> composite show better sensitivity (36.4 µA mM<sup>–1</sup> cm<sup>–2</sup>) and lower detection limit (0.005 mM) as well as the widest linear detection range (0.005–2 mM) compared to the other compositions. Thus, bacterial cellulose modified with conductive additives can be applied as a matrix for the immobilization of bacteria in microbial biosensors and microbial fuel cells.</p>\",\"PeriodicalId\":716,\"journal\":{\"name\":\"Nanotechnologies in Russia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-03-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanotechnologies in Russia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1134/s2635167623601493\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanotechnologies in Russia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s2635167623601493","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Engineering","Score":null,"Total":0}
BC/PEDOT:PSS/Graphene Conductive Composite As the Receptor Element for a Bacterial Biosensor
Тhe search for new materials with high conductivity and biocompatibility for use in biosensors is an important task. One promising material is bacterial cellulose (BC) due to its high surface area, high porosity, and biocompatibility. In this study, bacterial cellulose is modified with the PEDOT:PSS conductive gel and carbon nanomaterials to increase conductivity. Thermally expanded graphite/BC/PEDOT:PSS/graphene composition is used to immobilize Gluconobacter oxydans acetic-acid bacteria on the surface of a screen-printed carbon electrode. The effect of individual components of the composite on the catalytic activity of bacteria in the presence of 2,6-dichlorophenolindophenol redox mediator is studied. The addition of BC to the composition provides a higher stability of the electrode: the drop in signal within 35 days is 9%. A microbial biosensor based on TEG/PEDOT:PSS/Graphene/BC/G. oxydans composite show better sensitivity (36.4 µA mM–1 cm–2) and lower detection limit (0.005 mM) as well as the widest linear detection range (0.005–2 mM) compared to the other compositions. Thus, bacterial cellulose modified with conductive additives can be applied as a matrix for the immobilization of bacteria in microbial biosensors and microbial fuel cells.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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