循环自身抗体分析确定 LIMS1 是病理性近视中致病性自身免疫的潜在靶点。

IF 6.1 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS Molecular & Cellular Proteomics Pub Date : 2024-06-01 Epub Date: 2024-05-09 DOI:10.1016/j.mcpro.2024.100783
Jiao Qi, Hao Li, Yu Du, Yun Liu, Wenwen He, Jiaqi Meng, Ling Wei, Keke Zhang, Yi Lu, Xiangjia Zhu
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引用次数: 0

摘要

高度近视是全球致盲的主要原因,其中以典型近视性黄斑变性为特征的病理性近视危害最大。然而,其发病机制在很大程度上仍然未知。在此,我们利用 HuProt 阵列首先启动了高度近视的血清学自身抗体分析,并确定了 18 种潜在的自身抗体,其中抗 LIMS1 自身抗体通过定制的聚焦微阵列得到了验证。进一步的亚组分析表明,抗-LIMS1自身抗体与病理性近视有关,而不是与没有近视性黄斑变性的单纯高度近视有关。从机理上讲,抗 LIMS1 自身抗体主要属于 IgG1/IgG2/IgG3 亚类。病理近视患者血清中的IgG可通过细胞骨架紊乱和紧密连接成分减少破坏视网膜色素上皮细胞的屏障功能,还可引发视网膜色素上皮细胞中的促炎介质级联反应,而清除抗LIMS1自身抗体可减轻这些反应。这些数据共同揭示了病理近视中近视黄斑变性的自身免疫病因。
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Circulating Autoantibody Profiling Identifies LIMS1 as a Potential Target for Pathogenic Autoimmunity in pathologic Myopia.

High myopia is a leading cause of blindness worldwide, among which pathologic myopia, characterized by typical myopic macular degeneration, is the most detrimental. However, its pathogenesis remains largely unknown. Here, using a HuProt array, we first initiated a serological autoantibody profiling of high myopia and identified 18 potential autoantibodies, of which anti-LIMS1 autoantibody was validated by a customized focused microarray. Further subgroup analysis revealed its actual relevance to pathologic myopia, rather than simple high myopia without myopic macular degeneration. Mechanistically, anti-LIMS1 autoantibody predominantly belonged to IgG1/IgG2/IgG3 subclasses. Serum IgG obtained from patients with pathologic myopia could disrupt the barrier function of retinal pigment epithelial cells via cytoskeleton disorganization and tight junction component reduction, and also trigger a pro-inflammatory mediator cascade in retinal pigment epithelial cells, which were all attenuated by depletion of anti-LIMS1 autoantibody. Together, these data uncover a previously unrecognized autoimmune etiology of myopic macular degeneration in pathologic myopia.

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来源期刊
Molecular & Cellular Proteomics
Molecular & Cellular Proteomics 生物-生化研究方法
CiteScore
11.50
自引率
4.30%
发文量
131
审稿时长
84 days
期刊介绍: The mission of MCP is to foster the development and applications of proteomics in both basic and translational research. MCP will publish manuscripts that report significant new biological or clinical discoveries underpinned by proteomic observations across all kingdoms of life. Manuscripts must define the biological roles played by the proteins investigated or their mechanisms of action. The journal also emphasizes articles that describe innovative new computational methods and technological advancements that will enable future discoveries. Manuscripts describing such approaches do not have to include a solution to a biological problem, but must demonstrate that the technology works as described, is reproducible and is appropriate to uncover yet unknown protein/proteome function or properties using relevant model systems or publicly available data. Scope: -Fundamental studies in biology, including integrative "omics" studies, that provide mechanistic insights -Novel experimental and computational technologies -Proteogenomic data integration and analysis that enable greater understanding of physiology and disease processes -Pathway and network analyses of signaling that focus on the roles of post-translational modifications -Studies of proteome dynamics and quality controls, and their roles in disease -Studies of evolutionary processes effecting proteome dynamics, quality and regulation -Chemical proteomics, including mechanisms of drug action -Proteomics of the immune system and antigen presentation/recognition -Microbiome proteomics, host-microbe and host-pathogen interactions, and their roles in health and disease -Clinical and translational studies of human diseases -Metabolomics to understand functional connections between genes, proteins and phenotypes
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