添加锌的沙拉果(Salacca edulis Reinw.)内生酵母的生长分析

Nurul Kusmiyati, U. Utami, Muhammad Riefki Pratama, Liliek Harianie
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Biomassa tertinggi dihasilkan oleh YIS-4 yakni 4,13 g/300 mL, sedangkan jumlah sel tertinggi dihasilkan oleh YIS-3 yakni 27,84 x 106 sel/mL. Pada persentase pengembangan menunjukkan bahwa isolat khamir dengan perlakuan zinc 0,1 g/L membutuhkan waktu lebih lama untuk mencapai pengembangan tertinggi, namun volume roti setelah pemanggangan menunjukkan hasil yang lebih baik dibanding perlakuan kontrol. Isolat YIS-4 dengan perlakuan zinc 0,1 g/L menghasilkan volume roti tertinggi (949,54 cm3). Berdasarkan analisis organoleptik dengan parameter warna, aroma, rasa dan tekstur menunjukkan bahwa semua panelis lebih menyukai roti hasil fermentasi isolat YIS-4 dengan perlakuan zinc 0,1 g/L.AbstractThe endophytic yeast of salak fruit (Salacca edulis Reinw.) with codes YIS-3, YIS-4, and YIS-7 can be used for bread dough fermentation. This ability can be increased by adding a zinc source to the growth medium. 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引用次数: 0

摘要

通过 YIS-3、YIS-4 和 YIS-7 型发酵剂对鲑鱼(Salacca edulis Reinw.)在相关介质中添加锌元素可提高发酵效果。通过锌粉和发酵腐竹,可改善肥胖症患者的生活质量。挖掘的方法包括分离哈密瓜、在培养基中添加营养素、分析哈密瓜生物质、硒含量、哈密瓜体积和器官。对参数的分析结果表明,锌含量为 0.1 克/升的饵料可显著提高生物量和蛋白质的含量,与对照饵料的生物量和蛋白质含量相差无几。YIS-4 的生物量增加了 4.13 克/300 毫升,而 YIS-3 的硒增加了 27.84 x 106 sel/毫升。在持久性实验中,锌浓度为 0.1 克/升的哈密尔分离物可显著减少对持久性实验的影响,其体积与对照组相差无几。浓度为 0.1 克/升锌的 YIS-4 分离物的体积为 949.54 立方厘米。Abstract The endophytic yeast of salak fruit (Salacca edulis Reinw.) with codes YIS-3, YIS-4, and YIS-7 can be used for bread dough fermentation.在生长培养基中添加锌源可提高这种能力。本研究的目的是分析添加锌后沙拉科水果内生酵母的生长情况以及发酵面包的质量。采用的方法包括酵母分离物的复壮、在生长培养基中添加营养物质、分析酵母生物量、细胞计数、面团体积和感官。对生长参数的研究结果表明,添加 0.1 克/升锌的处理比对照处理的生物量和细胞数更高。生物量最高的是 YIS-4,为 4.13 g/300 mL,细胞数最多的是 YIS-3,为 27.84 x 106 cells/mL。膨胀率显示,用 0.1 g/L 锌处理的酵母分离物需要更长的时间才能达到最高膨胀率,但烘烤后的面包体积显示出比对照处理更好的效果。用 0.1 克/升锌处理的 YIS-4 分离物生产的面包体积最大(949.54 立方厘米)。根据色、香、味和口感参数进行的感官分析表明,所有小组成员都更喜欢经 0.1 克/升锌处理的 YIS-4 分离物发酵的面包。
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Analisis Pertumbuhan Khamir Endofit Buah Salak (Salacca edulis Reinw.) dengan Penambahan Zinc
AbstrakKhamir endofit buah salak (Salacca edulis Reinw.) dengan kode YIS-3, YIS-4, dan YIS-7 dapat dimanfaatkan untuk fermentasi adonan roti. Kemampuan tersebut dapat ditingkatkan dengan penambahan sumber zinc pada media pertumbuhannya. Tujuan penelitian ini untuk menganalisis pertumbuhan khamir endofit buah salak dengan penambahan zinc dan kualitas roti hasil fermentasi. Metode yang digunakan meliputi peremajaan isolat khamir, penambahan nutrisi pada media pertumbuhan, analisis biomassa khamir, jumlah sel, volume adonan, dan organoleptik. Hasil penelitian pada parameter pertumbuhan menunjukkan bahwa perlakuan dengan penambahan zinc 0,1 g/L menghasilkan biomassa dan jumlah sel lebih tinggi dibandingkan perlakuan kontrol. Biomassa tertinggi dihasilkan oleh YIS-4 yakni 4,13 g/300 mL, sedangkan jumlah sel tertinggi dihasilkan oleh YIS-3 yakni 27,84 x 106 sel/mL. Pada persentase pengembangan menunjukkan bahwa isolat khamir dengan perlakuan zinc 0,1 g/L membutuhkan waktu lebih lama untuk mencapai pengembangan tertinggi, namun volume roti setelah pemanggangan menunjukkan hasil yang lebih baik dibanding perlakuan kontrol. Isolat YIS-4 dengan perlakuan zinc 0,1 g/L menghasilkan volume roti tertinggi (949,54 cm3). Berdasarkan analisis organoleptik dengan parameter warna, aroma, rasa dan tekstur menunjukkan bahwa semua panelis lebih menyukai roti hasil fermentasi isolat YIS-4 dengan perlakuan zinc 0,1 g/L.AbstractThe endophytic yeast of salak fruit (Salacca edulis Reinw.) with codes YIS-3, YIS-4, and YIS-7 can be used for bread dough fermentation. This ability can be increased by adding a zinc source to the growth medium. The purpose of this study was to analyze the growth of the endophytic yeast of salak fruit with the addition of zinc and the quality of fermented bread. The methods used include rejuvenation of yeast isolates, addition of nutrients to growth media, analysis of yeast biomass, cell count, dough volume and organoleptic. The results of research on growth parameters showed that treatment with the addition of zinc 0.1 g/L resulted in higher biomass and cell count than the control treatment. The highest biomass was produced by YIS-4 which was 4.13 g/300 mL, while the highest number of cells was produced by YIS-3 which was 27.84 x 106 cells/mL. The swelling percentage showed that yeast isolates treated with 0.1 g/L zinc took longer to reach the highest swelling, but the volume of bread after baking showed better results than the control treatment. YIS-4 isolate treated with 0.1 g/L zinc produced the highest bread volume (949.54 cm3). Based on organoleptic analysis with parameters of color, aroma, taste and texture, it showed that all panelists preferred bread fermented YIS-4 isolate with 0.1 g/L zinc treatment.
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