Ali R. Jalalvand , Faezeh Majidi , Soheila Mohammadi , Sajad Fakhri , Cyrus Jalili , Tooraj Farshadnia
{"title":"测定甲状腺球蛋白以检测甲状腺癌治疗成功率的新型计算机辅助电化学生物传感器","authors":"Ali R. Jalalvand , Faezeh Majidi , Soheila Mohammadi , Sajad Fakhri , Cyrus Jalili , Tooraj Farshadnia","doi":"10.1016/j.sbsr.2024.100660","DOIUrl":null,"url":null,"abstract":"<div><p>In this work, a novel electrochemical thyroglobulin (Tg) biosensor was fabricated based on modification of a glassy carbon electrode with chitin-ionic liquid (Ch-IL) and gold nanoparticles (Au NPs). Surface of the Au NPs/Ch-IL/GCE was investigated and optimized by the image processing method. Drop-casting of the Ch-IL was performed onto the surface of Au NPs/Ch-IL/GCE to fabricate Ch-IL/Au NPs/Ch-IL/GCE. Then, sortilin (ST) was immobilized onto its surface by the use of glutaraldehyde. Biosensing of Tg in the range of Tg in the range of 0.1 to 8.5 fM was based on its interaction with the ST and change in hydrodynamic differential pulse voltammetric (DPV) response of the biosensor. The second-order calibration methods by U-PLS/RBL, N-PLS/RBL, MCR-ALS and PARAFAC2 were used to determination of Tg in the presence of thyroxine and triiodothyronine as uncalibrated interference, and our results confirmed the best performance was observed for exploiting second-order advantage by PARAFAC2 (sensitivity: 2.8 μA/fM, selectivity: 0.49, inverse of analytical sensitivity: 0.11 fM<sup>−1</sup>, limit of detection: 0.01fM) which was used to determination of Tg in human serum samples the presence of triiodothyronine and thyroxine as uncalibrated interference with the aim of detection of thyroid cancer treatment success. The ST/Ch-IL/Au NPs/Ch-IL/GCE assisted by PARAFAC2 was an excellent electronic device for medical diagnostic purposes.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180424000424/pdfft?md5=da1aa29f7f742b72b41c3de3c0ef9146&pid=1-s2.0-S2214180424000424-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A novel and computer assisted electrochemical biosensor for determination of thyroglobulin to detect thyroid cancer treatment success\",\"authors\":\"Ali R. Jalalvand , Faezeh Majidi , Soheila Mohammadi , Sajad Fakhri , Cyrus Jalili , Tooraj Farshadnia\",\"doi\":\"10.1016/j.sbsr.2024.100660\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this work, a novel electrochemical thyroglobulin (Tg) biosensor was fabricated based on modification of a glassy carbon electrode with chitin-ionic liquid (Ch-IL) and gold nanoparticles (Au NPs). Surface of the Au NPs/Ch-IL/GCE was investigated and optimized by the image processing method. Drop-casting of the Ch-IL was performed onto the surface of Au NPs/Ch-IL/GCE to fabricate Ch-IL/Au NPs/Ch-IL/GCE. Then, sortilin (ST) was immobilized onto its surface by the use of glutaraldehyde. Biosensing of Tg in the range of Tg in the range of 0.1 to 8.5 fM was based on its interaction with the ST and change in hydrodynamic differential pulse voltammetric (DPV) response of the biosensor. The second-order calibration methods by U-PLS/RBL, N-PLS/RBL, MCR-ALS and PARAFAC2 were used to determination of Tg in the presence of thyroxine and triiodothyronine as uncalibrated interference, and our results confirmed the best performance was observed for exploiting second-order advantage by PARAFAC2 (sensitivity: 2.8 μA/fM, selectivity: 0.49, inverse of analytical sensitivity: 0.11 fM<sup>−1</sup>, limit of detection: 0.01fM) which was used to determination of Tg in human serum samples the presence of triiodothyronine and thyroxine as uncalibrated interference with the aim of detection of thyroid cancer treatment success. The ST/Ch-IL/Au NPs/Ch-IL/GCE assisted by PARAFAC2 was an excellent electronic device for medical diagnostic purposes.</p></div>\",\"PeriodicalId\":424,\"journal\":{\"name\":\"Sensing and Bio-Sensing Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-05-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214180424000424/pdfft?md5=da1aa29f7f742b72b41c3de3c0ef9146&pid=1-s2.0-S2214180424000424-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensing and Bio-Sensing Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214180424000424\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensing and Bio-Sensing Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214180424000424","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A novel and computer assisted electrochemical biosensor for determination of thyroglobulin to detect thyroid cancer treatment success
In this work, a novel electrochemical thyroglobulin (Tg) biosensor was fabricated based on modification of a glassy carbon electrode with chitin-ionic liquid (Ch-IL) and gold nanoparticles (Au NPs). Surface of the Au NPs/Ch-IL/GCE was investigated and optimized by the image processing method. Drop-casting of the Ch-IL was performed onto the surface of Au NPs/Ch-IL/GCE to fabricate Ch-IL/Au NPs/Ch-IL/GCE. Then, sortilin (ST) was immobilized onto its surface by the use of glutaraldehyde. Biosensing of Tg in the range of Tg in the range of 0.1 to 8.5 fM was based on its interaction with the ST and change in hydrodynamic differential pulse voltammetric (DPV) response of the biosensor. The second-order calibration methods by U-PLS/RBL, N-PLS/RBL, MCR-ALS and PARAFAC2 were used to determination of Tg in the presence of thyroxine and triiodothyronine as uncalibrated interference, and our results confirmed the best performance was observed for exploiting second-order advantage by PARAFAC2 (sensitivity: 2.8 μA/fM, selectivity: 0.49, inverse of analytical sensitivity: 0.11 fM−1, limit of detection: 0.01fM) which was used to determination of Tg in human serum samples the presence of triiodothyronine and thyroxine as uncalibrated interference with the aim of detection of thyroid cancer treatment success. The ST/Ch-IL/Au NPs/Ch-IL/GCE assisted by PARAFAC2 was an excellent electronic device for medical diagnostic purposes.
期刊介绍：
Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies.
The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.
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