基于 28S rDNA 部分序列的南非林波波省 Aceria sp.

Q3 Agricultural and Biological Sciences Research on Crops Pub Date : 2024-05-24 DOI:10.31830/2348-7542.2024.roc-1030

Mehrnoush Aminisarteshnizi

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引用次数: 0

摘要

金合欢属（蝶形目：蝶形花科）是已知蝶形花科中最大的属，与经济上重要的农作物有关，因为害虫物种分布在世界各地。因此，有必要对其进行正确识别，以便对其进行控制和管理。本研究旨在分离和鉴定南非林波波省的 Aceria sp.。因此，这项分子研究于 2023 年在林波波大学进行。为了鉴定 Aceria sp.，使用了 28S rDNA 标记。使用 Chelex 方法提取 DNA。Nblast分析表明，南非的Aceria sp.与俄罗斯的一个种群（OP419491）有92%的相似性。利用最大似然法进行的系统发育分析将该物种与分子鉴定为 Aceria sp.的物种置于同一支系中，并得到高度支持（96）。总之，使用 28S rDNA 可以正确鉴定该物种。不过，为了更好地了解 Aceria sp.的系统发育，建议使用其他 DNA 标记。

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Phylogenetic position of Aceria sp. (Trombidiformes: Eriophyidae) based on 28S rDNA partial sequences Limpopo Province, South Africa

Aceria (Trombidiformes: Eriophyidae) is the largest known genus among the Eriophyoidea and is associated with economically important crops as pest species are distributed worldwide. Therefore, correctly identifying them for their control and management is necessary. This study was conducted to isolate and identify Aceria sp. in Limpopo Province, South Africa. Therefore, this molecular study was conducted in 2023 at Limpopo University. To identify Aceria sp., 28S rDNA marker used. The DNA was extracted using the Chelex method. The Aceria was identified as Aceria sp. The Nblast analysis showed South African Aceria sp. has 92% similarity with a population from Russia (OP419491). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as Aceria sp. in the same clade with highly supported (96) bootstrap values. In conclusion, this species is identified using 28S rDNA properly. However, other DNA markers are recommended to better understand Aceria sp. phylogeny.

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来源期刊

Research on Crops Agricultural and Biological Sciences-Soil Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Research on Crops is a peer-reviewed journal publishing original research papers, review articles and short communications in English on all basic and applied aspects of crop sciences, agricultural water management, agro-climatology, agroforestry, agronomy, crop production, crop protection, cropping systems, food science & technology, genetics & plant breeding, horticulture, plant & soil science, plant biotechnology, plant nutrition, post-harvest management of crops, seed science, soil management & tillage, vegetables, weed science, agricultural engineering, agri-business, agricultural economics and extension, etc. The aim of the journal is to provide a forum for the scientific community to publish their latest research findings. The manuscripts submitted for publication should not contain data older than 4 years on the date of submission. The articles submitted for publication in this journal should not be submitted elsewhere simultaneously for publication in another journal. These should not carry any copyright material without prior permission of copyright holder. The articles should present a complete picture of the investigation made and should not be split into parts. There is no prescribed limit regarding the number of pages in case of full-length articles. However, the authors are advised to keep the length of their articles from 4 to 10 full printed pages of the journal. The articles should be divided into the sub-sections: ABSTRACT, INTRODUCTION, MATERIALS AND METHODS, RESULTS AND DISCUSSION, CONCLUSIONS, and REFERENCES. Tables and figures should be appended separately at the end.