R.K. Verma, R. K. Joshi, N. Joshi, J. Singh, A. Prajapati
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引用次数: 0
摘要
背景：耐甲氧西林金黄色葡萄球菌（MRSA）感染是导致动物和人类感染的主要原因之一，与严重的发病率、死亡率、住院时间和成本负担有关。研究方法本研究随机从牛、水牛、绵羊和山羊身上采集了 725 份鼻拭子和牛奶样本，将这些样本接种到混合了奥沙西林抗性选择性添加剂的甘露醇盐琼脂上，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和聚合酶链反应（PCR）测试对 MRSA 分离物进行分子鉴定。结果显示在 725 个样本中，发现 171 个样本对奥沙西林耐药金黄色葡萄球菌呈阳性。SDS-PAGE 显示了分子量为 13、17、20、24、26、28、33、36、39、43、47、59、64、72、86、97 和 121 kDa 的不同条带。在分离物中检测到了 533 碱基对（bp）的扩增 mec A DNA 片段（137 个分离物）和 510 bp 的 fem A（133 个分离物）PCR 产物。
Molecular Characterization of Methicillin-resistant Staphylococcus aureus (MRSA) Isolates from Ruminants
Background: Methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the leading causes of infections in animals as well as human beings and is associated with significant morbidity, mortality, length of stay and cost burden. Methods: In this study, 725 samples of nasal swabs and milk were collected randomly from cattle, buffalo, sheep and goats and these samples were inoculated on Mannitol salt agar mixed with Oxacillin Resistance Selective Supplement for the molecular characterization of MRSA isolates through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and polymerase chain reaction (PCR) test. Result: Out of 725 samples, 171 were found positive for Oxacillin resistant S. aureus. The SDS-PAGE showed different bands of molecular weight 13, 17, 20, 24, 26, 28, 33, 36, 39, 43, 47, 59, 64, 72, 86, 97 and 121 kDa. An amplified mec A DNA fragment (137) of 533 base pairs (bp) and PCR product of fem A (133 isolates) of 510 bp were detected in isolates.
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