新型褪黑素芽孢杆菌 EH143 可减轻大豆的盐和镉胁迫。

IF 8.3 1区 医学 Q1 ENDOCRINOLOGY & METABOLISM Journal of Pineal Research Pub Date : 2024-05-27 DOI:10.1111/jpi.12957
Eun-Hae Kwon, Arjun Adhikari, Muhammad Imran, Adil Hussain, Ho-Jun Gam, Ji-In Woo, Jin Ryeol Jeon, Da-Sol Lee, Chung-Yeol Lee, Liny Lay, Sang-Mo Kang, Won-Chan Kim, Byung-Wook Yun, In-Jung Lee
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引用次数: 0

摘要

最近,微生物和外源褪黑激素的应用被认为是提高农作物耐盐性和重金属解毒能力的有效生物工具。因此,本研究的目标是分离和评估一种新型褪黑激素生产植物生长促进菌。通过高通量全基因组测序、植物激素测定、表达谱分析和生化分析,我们可以鉴定出一种能产生褪黑激素的新型 PGPB,并揭示它是如何促进大豆生长发育并抵御盐和镉胁迫的。我们确定了耐盐(NaCl > 800 mM)和抗重金属(Cd > 3 mM)根瘤菌 Bacillus safensis EH143 的褪黑激素合成途径(色氨酸→色胺→serotonin 褪黑激素),并用它来处理受到 Cd 和 NaCl 胁迫的大豆植株。结果表明,EH143 对重金属有很高的生物积累能力,能显著提高大豆对 P、Ca2+ 的吸收和 K+/Na+(在盐胁迫下为 93%↑)的比率,同时降低大豆嫩芽对 Cd 的吸收(在 Cd 胁迫下为 49%)。离子调节因子 HKT1、MYPB67 以及钙传感器 CDPK5 和 CaMK1 的表达支持了这种活性,最终导致植物生长速度加快。在对照、盐和镉处理的植株中,EH143 可使嫩枝中的 ABA 含量分别明显减少 13%、20% 和 34%,并使嫩枝中的 SA 生物合成量分别增加 14.8%、31% 和 48.2%,同时上调 CYP707A1 和 CYP707A2 以及 PAL1 和 ICS。经 EH143 处理后,褪黑激素含量明显降低,ASMT3 的表达量也有所减少;此外,在盐+镉胁迫下,过氧化物酶(POD)和超氧化物歧化酶(SOD)的表达量分别减少了 134.5% 和 39%,总氨基酸的含量也有所增加。对 EH143 进行的全基因组测序和注释发现了褪黑激素前体色氨酸合成酶(trpA、trpB、trpS)、金属和其他离子调节剂(镉:cadA,钾:KtrA 和 KtrB,镉:cadA,钾:cadA 和 KtrB)的存在:此外,EH143 还提供了可能参与植物代谢系统编程的酶激活剂(包括苷元转运蛋白 yfiZ 和 yfhA、SOD sodA、过氧化氢酶 katA1 和谷胱甘肽调节剂 KefG)。因此,EH143 能显著降低盐+镉胁迫下植物的脂质过氧化物(O2-、MDA 和 H2O2)含量,降幅分别高达 69%、46% 和 29%。这些研究结果表明,EH143 是一种有效的生物肥料,可以减轻作物的氯化钠和镉毒性,并可替代外源褪黑激素的应用。
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Novel melatonin-producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Recently, microorganism and exogenous melatonin application has been recognized as an efficient biological tool for enhancing salt tolerance and heavy metal detoxification in agriculture crops. Thus, the goal of this study was to isolate and evaluate a novel melatonin-producing plant growth promoting bacterium. With high-throughput whole genome sequencing, phytohormone measurements, expression profiling, and biochemical analysis, we can identify a novel PGPB that produces melatonin and unravel how it promotes soybean growth and development and protects against salt and Cd stress. We identify the melatonin synthesis pathway (tryptophan→tryptamine→serotonin melatonin) of the halotolerant (NaCl > 800 mM) and heavy metal-resistant (Cd >3 mM) rhizobacterium Bacillus safensis EH143 and use it to treat soybean plants subjected to Cd and NaCl stresses. Results show that EH143 will highly bioaccumulate heavy metals and significantly improve P and Ca2+ uptake and the K+/Na+ (93%↑under salt stress) ratio while reducing Cd uptake (49% under Cd stress) in shoots. This activity was supported by the expression of the ion regulator HKT1, MYPB67, and the calcium sensors CDPK5 and CaMK1 which ultimately led to increased plant growth. EH143 significantly decreased ABA content in shoots by 13%, 20%, and 34% and increased SA biosynthesis in shoots by 14.8%, 31%, and 48.2% in control, salt, and Cd-treated plants, upregulating CYP707A1 and CYP707A2 and PAL1 and ICS, respectively. The melatonin content significantly decreased along with a reduced expression of ASMT3 following treatment with EH143; moreover, reduced expression of peroxidase (POD) and superoxide dismutase (SOD) by 134.5% and 39% under salt+Cd stress, respectively and increased level of total amino acids were observed. Whole-genome sequencing and annotation of EH143 revealed the presence of the melatonin precursor tryptophan synthase (trpA, trpB, trpS), metal and other ion regulators (Cd: cadA, potassium: KtrA and KtrB, phosphate: glpT, calcium: yloB, the sodium/glucose cotransporter: sgIT, and the magnesium transporter: mgtE), and enzyme activators (including the siderophore transport proteins yfiZ and yfhA, the SOD sodA, the catalase katA1, and the glutathione regulator KefG) that may be involved in programming the plant metabolic system. As a consequence, EH143 treatment significantly reduced the contents of lipid peroxidation (O2-, MDA, and H2O2) up to 69%, 46%, and 29% in plants under salt+Cd stress, respectively. These findings suggest that EH143 could be a potent biofertilizer to alleviate NaCl and Cd toxicity in crops and serve as an alternative substitute for exogenous melatonin application.

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来源期刊
Journal of Pineal Research
Journal of Pineal Research 医学-内分泌学与代谢
CiteScore
17.70
自引率
4.90%
发文量
66
审稿时长
1 months
期刊介绍: The Journal of Pineal Research welcomes original scientific research on the pineal gland and melatonin in vertebrates, as well as the biological functions of melatonin in non-vertebrates, plants, and microorganisms. Criteria for publication include scientific importance, novelty, timeliness, and clarity of presentation. The journal considers experimental data that challenge current thinking and welcomes case reports contributing to understanding the pineal gland and melatonin research. Its aim is to serve researchers in all disciplines related to the pineal gland and melatonin.
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Cover image RETRACTION: Effects of Melatonin on Fatty Liver Disease: The Role of NR4A1/DNA-Pkcs/P53 Pathway, Mitochondrial Fission, and Mitophagy From Seasonality to Species Conservation: Chronobiological Research on European Hamsters in Strasbourg, France Melatonin Inhibits ET-1 Production to Break Crosstalk Between Prostate Cancer and Bone Cells: Implication for Osteoblastic Bone Metastasis Treatment Issue Information
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