幽门螺旋杆菌感染期间血液和胃黏膜中的 CCR6+ T 辅助细胞和调节性 T 细胞。

IF 4.3 2区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Helicobacter Pub Date : 2024-05-31 DOI:10.1111/hel.13097
Vladimir Talayev, Maria Svetlova, Irina Zaichenko, Elena Voronina, Olga Babaykina, Natalia Neumoina, Ksenia Perfilova
{"title":"幽门螺旋杆菌感染期间血液和胃黏膜中的 CCR6+ T 辅助细胞和调节性 T 细胞。","authors":"Vladimir Talayev,&nbsp;Maria Svetlova,&nbsp;Irina Zaichenko,&nbsp;Elena Voronina,&nbsp;Olga Babaykina,&nbsp;Natalia Neumoina,&nbsp;Ksenia Perfilova","doi":"10.1111/hel.13097","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p><i>Helicobacter pylori</i> (<i>H. pylori</i>) can evade the host's immune response and persist for a long time on the gastric mucosa. T helper (Th) cells appear to be involved in the control of <i>H. pylori</i> bacteria but promote mucosal inflammation. In contrast, regulatory T cells (Tregs) may reduce inflammation but promote <i>H. pylori</i> persistence. CC motif chemokine receptor 6 (CCR6) is involved in the migration of various cells into inflamed gastric mucosa. In this study, we examined CCR6<sup>+</sup> Th cells and CCR6<sup>+</sup> Tregs during <i>H. pylori</i> infection in humans.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>Isolation of cells from blood and mucosal biopsies, magnetic separation of В cells, CD4<sup>+</sup> and CD4<sup>+</sup>CCR6<sup>+</sup>CD45RO<sup>+</sup> T cells, antigen-specific activation, B cell response in vitro, flow cytometry, determination of CD4<sup>+</sup>CD25<sup>hi</sup>FoxP3<sup>+</sup> Tregs and various groups of Th cells.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>CD4<sup>+</sup>CCR6<sup>+</sup> blood lymphocytes from healthy donors included Th cells and Tregs. These CCR6<sup>+</sup> Th cells produced proinflammatory cytokines and also stimulated plasma cell maturation and antibody production in vitro. <i>H. pylori</i> gastritis and peptic ulcer disease were associated with an increase in the number of circulate CD4<sup>+</sup>CCR6<sup>+</sup>CD45RO<sup>+</sup> cells and the percentage of Th1, Th17 and Th1/17 cells in this lymphocyte subgroup. In <i>H. pylori</i>-positive patients, circulating CD4<sup>+</sup>CCR6<sup>+</sup> cells contained a higher proportion of <i>H. pylori</i>-specific cells compared with their CD4<sup>+</sup>CCR6<sup>−</sup> counterparts. <i>H. pylori</i> infection strongly increased the content of CD4<sup>+</sup> lymphocytes in the inflamed gastric mucosa, with the majority of these CD4<sup>+</sup> lymphocytes expressing CCR6. CD4<sup>+</sup>CCR6<sup>+</sup> lymphocytes from <i>H. pylori-</i>infected stomach included Tregs and in vivo activated T cells, some of which produced interferon-γ without ex vivo stimulation.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p><i>H. pylori</i> infection causes an increase in the number of mature CD4<sup>+</sup>CCR6<sup>+</sup> lymphocytes in the blood, with a pro-inflammatory shift in their composition and enrichment of the gastric mucosa with CD4<sup>+</sup>CCR6<sup>+</sup> lymphocytes, including CCR6<sup>+</sup> Th1 cells and Tregs.</p>\n </section>\n </div>","PeriodicalId":13223,"journal":{"name":"Helicobacter","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CCR6+ T helper cells and regulatory T cells in the blood and gastric mucosa during Helicobacter pylori infection\",\"authors\":\"Vladimir Talayev,&nbsp;Maria Svetlova,&nbsp;Irina Zaichenko,&nbsp;Elena Voronina,&nbsp;Olga Babaykina,&nbsp;Natalia Neumoina,&nbsp;Ksenia Perfilova\",\"doi\":\"10.1111/hel.13097\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p><i>Helicobacter pylori</i> (<i>H. pylori</i>) can evade the host's immune response and persist for a long time on the gastric mucosa. T helper (Th) cells appear to be involved in the control of <i>H. pylori</i> bacteria but promote mucosal inflammation. In contrast, regulatory T cells (Tregs) may reduce inflammation but promote <i>H. pylori</i> persistence. CC motif chemokine receptor 6 (CCR6) is involved in the migration of various cells into inflamed gastric mucosa. In this study, we examined CCR6<sup>+</sup> Th cells and CCR6<sup>+</sup> Tregs during <i>H. pylori</i> infection in humans.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>Isolation of cells from blood and mucosal biopsies, magnetic separation of В cells, CD4<sup>+</sup> and CD4<sup>+</sup>CCR6<sup>+</sup>CD45RO<sup>+</sup> T cells, antigen-specific activation, B cell response in vitro, flow cytometry, determination of CD4<sup>+</sup>CD25<sup>hi</sup>FoxP3<sup>+</sup> Tregs and various groups of Th cells.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>CD4<sup>+</sup>CCR6<sup>+</sup> blood lymphocytes from healthy donors included Th cells and Tregs. These CCR6<sup>+</sup> Th cells produced proinflammatory cytokines and also stimulated plasma cell maturation and antibody production in vitro. <i>H. pylori</i> gastritis and peptic ulcer disease were associated with an increase in the number of circulate CD4<sup>+</sup>CCR6<sup>+</sup>CD45RO<sup>+</sup> cells and the percentage of Th1, Th17 and Th1/17 cells in this lymphocyte subgroup. In <i>H. pylori</i>-positive patients, circulating CD4<sup>+</sup>CCR6<sup>+</sup> cells contained a higher proportion of <i>H. pylori</i>-specific cells compared with their CD4<sup>+</sup>CCR6<sup>−</sup> counterparts. <i>H. pylori</i> infection strongly increased the content of CD4<sup>+</sup> lymphocytes in the inflamed gastric mucosa, with the majority of these CD4<sup>+</sup> lymphocytes expressing CCR6. CD4<sup>+</sup>CCR6<sup>+</sup> lymphocytes from <i>H. pylori-</i>infected stomach included Tregs and in vivo activated T cells, some of which produced interferon-γ without ex vivo stimulation.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p><i>H. pylori</i> infection causes an increase in the number of mature CD4<sup>+</sup>CCR6<sup>+</sup> lymphocytes in the blood, with a pro-inflammatory shift in their composition and enrichment of the gastric mucosa with CD4<sup>+</sup>CCR6<sup>+</sup> lymphocytes, including CCR6<sup>+</sup> Th1 cells and Tregs.</p>\\n </section>\\n </div>\",\"PeriodicalId\":13223,\"journal\":{\"name\":\"Helicobacter\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-05-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Helicobacter\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/hel.13097\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GASTROENTEROLOGY & HEPATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Helicobacter","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/hel.13097","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:幽门螺杆菌(H. pylori)可以逃避宿主的免疫反应,并在胃粘膜上长期存在。T 辅助(Th)细胞似乎参与了对幽门螺杆菌的控制,但会促进粘膜炎症。与此相反,调节性 T 细胞(Tregs)可能会减轻炎症,但会促进幽门螺杆菌的持续存在。CC motif趋化因子受体 6(CCR6)参与了各种细胞向发炎胃粘膜的迁移。在这项研究中,我们检测了幽门螺杆菌感染过程中的 CCR6+ Th 细胞和 CCR6+ Tregs:从血液和粘膜活检组织中分离细胞,磁性分离В细胞、CD4+和CD4+CCR6+CD45RO+ T细胞,抗原特异性活化,体外B细胞反应,流式细胞术,测定CD4+CD25hiFoxP3+ Tregs和各种Th细胞群:结果:来自健康捐献者的 CD4+CCR6+ 血液淋巴细胞包括 Th 细胞和 Tregs。这些 CCR6+ Th 细胞能产生促炎细胞因子,还能刺激体外浆细胞成熟和抗体产生。幽门螺杆菌胃炎和消化性溃疡病与循环 CD4+CCR6+CD45RO+ 细胞数量的增加以及该淋巴细胞亚群中 Th1、Th17 和 Th1/17 细胞比例的增加有关。在幽门螺杆菌阳性患者中,循环 CD4+CCR6+ 细胞中幽门螺杆菌特异性细胞的比例高于 CD4+CCR6- 细胞。幽门螺杆菌感染大大增加了发炎胃黏膜中 CD4+ 淋巴细胞的含量,其中大部分 CD4+ 淋巴细胞表达 CCR6。幽门螺杆菌感染胃中的 CD4+CCR6+ 淋巴细胞包括 Tregs 和体内活化的 T 细胞,其中一些无需体内外刺激即可产生干扰素-γ:结论:幽门螺杆菌感染会导致血液中成熟的 CD4+CCR6+ 淋巴细胞数量增加,其组成发生促炎性变化,并使胃黏膜中的 CD4+CCR6+ 淋巴细胞富集,其中包括 CCR6+ Th1 细胞和 Tregs。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
CCR6+ T helper cells and regulatory T cells in the blood and gastric mucosa during Helicobacter pylori infection

Background

Helicobacter pylori (H. pylori) can evade the host's immune response and persist for a long time on the gastric mucosa. T helper (Th) cells appear to be involved in the control of H. pylori bacteria but promote mucosal inflammation. In contrast, regulatory T cells (Tregs) may reduce inflammation but promote H. pylori persistence. CC motif chemokine receptor 6 (CCR6) is involved in the migration of various cells into inflamed gastric mucosa. In this study, we examined CCR6+ Th cells and CCR6+ Tregs during H. pylori infection in humans.

Materials and Methods

Isolation of cells from blood and mucosal biopsies, magnetic separation of В cells, CD4+ and CD4+CCR6+CD45RO+ T cells, antigen-specific activation, B cell response in vitro, flow cytometry, determination of CD4+CD25hiFoxP3+ Tregs and various groups of Th cells.

Results

CD4+CCR6+ blood lymphocytes from healthy donors included Th cells and Tregs. These CCR6+ Th cells produced proinflammatory cytokines and also stimulated plasma cell maturation and antibody production in vitro. H. pylori gastritis and peptic ulcer disease were associated with an increase in the number of circulate CD4+CCR6+CD45RO+ cells and the percentage of Th1, Th17 and Th1/17 cells in this lymphocyte subgroup. In H. pylori-positive patients, circulating CD4+CCR6+ cells contained a higher proportion of H. pylori-specific cells compared with their CD4+CCR6 counterparts. H. pylori infection strongly increased the content of CD4+ lymphocytes in the inflamed gastric mucosa, with the majority of these CD4+ lymphocytes expressing CCR6. CD4+CCR6+ lymphocytes from H. pylori-infected stomach included Tregs and in vivo activated T cells, some of which produced interferon-γ without ex vivo stimulation.

Conclusion

H. pylori infection causes an increase in the number of mature CD4+CCR6+ lymphocytes in the blood, with a pro-inflammatory shift in their composition and enrichment of the gastric mucosa with CD4+CCR6+ lymphocytes, including CCR6+ Th1 cells and Tregs.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Helicobacter
Helicobacter 医学-微生物学
CiteScore
8.40
自引率
9.10%
发文量
76
审稿时长
2 months
期刊介绍: Helicobacter is edited by Professor David Y Graham. The editorial and peer review process is an independent process. Whenever there is a conflict of interest, the editor and editorial board will declare their interests and affiliations. Helicobacter recognises the critical role that has been established for Helicobacter pylori in peptic ulcer, gastric adenocarcinoma, and primary gastric lymphoma. As new helicobacter species are now regularly being discovered, Helicobacter covers the entire range of helicobacter research, increasing communication among the fields of gastroenterology; microbiology; vaccine development; laboratory animal science.
期刊最新文献
Efficacy of Bismuth Quadruple Therapy in the Treatment of Helicobacter pylori-Infected Peptic Ulcer Children in Vietnam Map-Like Redness Development After Eradication Therapy for Helicobacter pylori Infection: Prospective Multicenter Observational Study Issue Information Correction to Antimicrobial Resistance of Helicobacter pylori Isolated From Latin American Children and Adolescents (2008–2023): A Systematic Review Influence of oipA Phase Variation on Virulence Phenotypes Related to Type IV Secretion System in Helicobacter pylori
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1