生物打印唾液腺模型及其再生应用。

IF 2.5 Q2 DENTISTRY, ORAL SURGERY & MEDICINE BDJ Open Pub Date : 2024-05-30 DOI:10.1038/s41405-024-00219-2
Jutapak Klangprapan, Glauco R Souza, João N Ferreira
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引用次数: 0

摘要

目的:唾液腺(SG)功能减退是头颈部癌症放疗后常见的临床症状。放射线通常会破坏唾液腺的分泌棘突,使唾液腺的再生潜力受到限制。由于SG棘突和导管的结构复杂,三维(3D)生物打印平台应运而生,可在空间上确定这些体外上皮细胞单位,并开发用于再生的微型器官或器官组织。由于证据有限,本综述重点介绍了用于 SG 再生的生物打印平台的优势和挑战:方法:有人提出了SG微组织工程策略,如细胞的磁性三维生物组装和含有细胞的微纤维和微管的微流体同轴三维生物打印,以取代受损的尖状体单位,避免使用异种基质(如Matrigel),并恢复唾液流动:替代 SG 受损器官具有挑战性,因为其结构复杂,结合了导管网络和针状上皮单元,以促进唾液的单向流动。我们的研究小组是第一个开发出具有神经支配功能的三维生物组装 SG 上皮功能器官组织的小组。最近,利用水凝胶微纤维和微管的同轴三维生物打印技术进行的微组织工程也支持形成有活力的上皮细胞。这两种生物打印方法都能克服对 Matrigel 的需求,促进成人干细胞(如人类牙髓干细胞)和原代 SG 细胞组装成微型三维构建体,这些构建体能产生自己的基质,并自我组织成具有腔化区域的微型模块化组织集群。此外,还设计了细胞外囊泡(EV)疗法,并在体内外对辐射损伤后的SG再生进行了验证:结论:磁性三维生物组装和微流控同轴生物打印平台有可能通过类器官移植或类器官衍生的EV疗法创建用于再生应用的SG小器官。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Bioprinting salivary gland models and their regenerative applications.

Objective: Salivary gland (SG) hypofunction is a common clinical condition arising from radiotherapy to suppress head and neck cancers. The radiation often destroys the SG secretory acini, and glands are left with limited regenerative potential. Due to the complex architecture of SG acini and ducts, three-dimensional (3D) bioprinting platforms have emerged to spatially define these in vitro epithelial units and develop mini-organs or organoids for regeneration. Due to the limited body of evidence, this comprehensive review highlights the advantages and challenges of bioprinting platforms for SG regeneration.

Methods: SG microtissue engineering strategies such as magnetic 3D bioassembly of cells and microfluidic coaxial 3D bioprinting of cell-laden microfibers and microtubes have been proposed to replace the damaged acinar units, avoid the use of xenogeneic matrices (like Matrigel), and restore salivary flow.

Results: Replacing the SG damaged organ is challenging due to its complex architecture, which combines a ductal network with acinar epithelial units to facilitate a unidirectional flow of saliva. Our research group was the first to develop 3D bioassembly SG epithelial functional organoids with innervation to respond to both cholinergic and adrenergic stimulation. More recently, microtissue engineering using coaxial 3D bioprinting of hydrogel microfibers and microtubes could also supported the formation of viable epithelial units. Both bioprinting approaches could overcome the need for Matrigel by facilitating the assembly of adult stem cells, such as human dental pulp stem cells, and primary SG cells into micro-sized 3D constructs able to produce their own matrix and self-organize into micro-modular tissue clusters with lumenized areas. Furthermore, extracellular vesicle (EV) therapies from organoid-derived secretome were also designed and validated ex vivo for SG regeneration after radiation damage.

Conclusion: Magnetic 3D bioassembly and microfluidic coaxial bioprinting platforms have the potential to create SG mini-organs for regenerative applications via organoid transplantation or organoid-derived EV therapies.

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来源期刊
BDJ Open
BDJ Open Dentistry-Dentistry (all)
CiteScore
3.70
自引率
3.30%
发文量
34
审稿时长
30 weeks
期刊最新文献
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