铁蛋白沉积参与了达尔盐敏感性高血压大鼠心脏和肾脏组织的纤维化。

IF 3.2 3区 医学 Q2 PERIPHERAL VASCULAR DISEASE American Journal of Hypertension Pub Date : 2024-09-16 DOI:10.1093/ajh/hpae076
Ya-Qi Huang, Kuang Peng, Jun Yan, Hui-Lin Chen, Pei-Yong Jiang, Ya-Fang Du, Xiang Ling, Si-Liang Zhang, Jie Wu
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引用次数: 0

摘要

背景:盐敏感性高血压通常更容易诱发心脏和肾脏等靶器官的损伤。因此,我们推测铁蛋白沉积与盐敏感性高血压的器官损伤密切相关。本研究旨在探讨铁蛋白沉积是否参与盐敏感性高血压大鼠心肌纤维化和肾脏纤维化的发生和发展:方法:10 只 7 周大的雄性 Dahl 盐敏感(Dahl-SS)大鼠适应性喂养 1 周,然后随机分为两组,分别喂养正常饮食(0.3% NaCl,NDS 组)或高盐饮食(8% NaCl,HDS 组)8 周。观察大鼠的血压,并通过 HE 染色、Masson 染色、普鲁士蓝染色、TEM、组织铁含量检测、MDA 含量检测、免疫荧光和 Western 印迹对 Dahl-SS 大鼠的心脏和肾脏进行分析:结果:与 NDS 组大鼠相比,HDS 组大鼠收缩压(SBP)和舒张压(DBP)均升高(PC结论:铁色素沉着参与了大鼠心血管疾病的发生:铁蛋白沉积参与了盐敏感性高血压大鼠心脏和肾脏组织的损伤和纤维化。
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The Participation of Ferroptosis in Fibrosis of the Heart and Kidney Tissues in Dahl Salt-Sensitive Hypertensive Rats.

Background: Salt-sensitive hypertension is often more prone to induce damage to target organs such as the heart and kidneys. Abundant recent studies have demonstrated a close association between ferroptosis and cardiovascular diseases. Therefore, we hypothesize that ferroptosis may be closely associated with organ damage in salt-sensitive hypertension. This study aimed to investigate whether ferroptosis is involved in the occurrence and development of myocardial fibrosis and renal fibrosis in salt-sensitive hypertensive rats.

Methods: Ten 7-week-old male Dahl salt-sensitive (Dahl-SS) rats were adaptively fed for 1 week, then randomly divided into two groups and fed either a normal diet (0.3% NaCl, normal diet group) or a high-salt diet (8% NaCl, high-salt diet group) for 8 weeks. Blood pressure of the rats was observed, and analysis of the hearts and kidneys of Dahl-SS rats was conducted via hematoxylin-eosin (HE) staining, Masson staining, Prussian blue staining, transmission electron microscopy, tissue iron content detection, malondialdehyde content detection, immunofluorescence, and Western blot.

Results: Compared to the normal diet group, rats in the high-salt diet group had increases in systolic blood pressure and diastolic blood pressure (P < 0.05); collagen fiber accumulation was observed in the heart and kidney tissues (P < 0.01), accompanied by alterations in mitochondrial ultrastructure, reduced mitochondrial volume, and increased density of the mitochondrial double membrane. Additionally, there were significant increases in both iron content and malondialdehyde levels (P < 0.05). Immunofluorescence and Western blot results both indicated significant downregulation (P < 0.05) of xCT and GPX4 proteins associated with ferroptosis in the high-salt diet group.

Conclusions: Ferroptosis is involved in the damage and fibrosis of the heart and kidney tissues in salt-sensitive hypertensive rats.

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来源期刊
American Journal of Hypertension
American Journal of Hypertension 医学-外周血管病
CiteScore
6.90
自引率
6.20%
发文量
144
审稿时长
3-8 weeks
期刊介绍: The American Journal of Hypertension is a monthly, peer-reviewed journal that provides a forum for scientific inquiry of the highest standards in the field of hypertension and related cardiovascular disease. The journal publishes high-quality original research and review articles on basic sciences, molecular biology, clinical and experimental hypertension, cardiology, epidemiology, pediatric hypertension, endocrinology, neurophysiology, and nephrology.
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