间歇性缺氧训练对氧气依赖过程的适应性影响,是一种潜在的治疗策略工具。

IF 2.5 Q3 CELL BIOLOGY Cellular Physiology and Biochemistry Pub Date : 2024-06-10 DOI:10.33594/000000705
Natalia Kurhaluk, Oleksandr Lukash, Piotr Kamiński, Halina Tkaczenko
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引用次数: 0

摘要

背景/目的:间歇性缺氧训练(IHT)作为增强不同病理状态(其中以急性缺氧为主)下适应潜能的有效工具,具有重要的益处。因此,我们的研究旨在评估一氧化氮系统(亚硝酸盐、硝酸盐、碳酰胺和多胺总含量)对心脏和肝脏线粒体中 ADP 刺激的耗氧量和氧化磷酸化以及血液中氧化应激生物标志物的影响机制、大鼠暴露于 IHT 法和急性缺氧,并在每次 IHT 前接受氨基酸 L-精氨酸(600 毫克/千克,30 分钟)或 NO 合酶抑制剂 L-NNA(35 毫克/千克,30 分钟)处理。方法我们分析了 IHT 期间组织中氧依赖过程系统的调节情况(用氧图法分析线粒体呼吸,用生化方法分析微粒体氧化和脂过氧化过程),以及同时服用 L-精氨酸后形成的短期和长期效应(最后一次 IHT 治疗后 30、60 和 180 天)。我们特别研究了间歇性缺氧期间线粒体功能是如何通过氧化底物(琥珀酸或α-酮戊二酸)在细胞稳定和适应的生物能机制中进行调节的:通过测量大鼠血浆(近 7 倍)和红细胞(超过 7 倍)中一氧化氮的稳定代谢物亚硝酸阴离子的水平,可以发现 IHT 法与内源性一氧化氮的产生显著增加有关。在心脏和肝脏的能量供应过程中,一氧化氮依赖性代谢转化途径的加强,伴随着间歇模式下的振荡适应机制,导致血浆和肝脏中尿素和多胺的产生可能减少,但红细胞中的尿素和多胺的产生没有减少。在进行间歇疗法之前服用左旋精氨酸可提高一氧化氮循环中亚硝酸盐还原成分的水平,这种作用可持续到实验结束后的 180 天:因此,本研究中显示的 IHT 及其亚硝酸盐依赖成分的功效与长期适应性反应的形成有关,它通过防止组织中的脂过氧化过程加剧,使抗氧化防御的主要酶发生明显变化,并稳定红细胞膜,这对整个氧依赖过程的调节系统具有明显的保护作用。
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Adaptive Effects of Intermittent Hypoxia Training on Oxygen-Dependent Processes as a Potential Therapeutic Strategy Tool.

Background/aims: Important benefits of intermittent hypoxic training (IHT) have emerged as an effective tool for enhancing adaptive potential in different pathological states, among which acute hypoxia dominates. Therefore, the aim of our study was to evaluate the mechanisms related to the effects of the nitric oxide system (nitrites, nitrates, carbamide, and total polyamine content) on ADP-stimulated oxygen consumption and oxidative phosphorylation in heart and liver mitochondria and biomarkers of oxidative stress in the blood, heart, and liver of rats exposed to the IHT method and acute hypoxia and treated with the amino acid L-arginine (600 mg/kg, 30 min) or the NO synthase inhibitor L-NNA (35 mg/kg, 30 min) prior to each IHT session.

Methods: We analysed the modulation of the system of oxygen-dependent processes (mitochondrial respiration with the oxygraphic method, microsomal oxidation, and lipoperoxidation processes using biochemical methods) in tissues during IHT in the formation of short-term and long-term effects (30, 60, and 180 days after the last IHT session) with simultaneous administration of L-arginine. In particular, we investigated how mitochondrial functions are modulated during intermittent hypoxia with the use of oxidation substrates (succinate or α-ketoglutarate) in bioenergetic mechanisms of cellular stability and adaptation.

Results: The IHT method is associated with a significant increase in the production of endogenous nitric oxide measured by the levels of its stable metabolite, nitrite anion, in both plasma (almost 7-fold) and erythrocytes (more than 7-fold) of rats. The intensification of nitric oxide-dependent pathways of metabolic transformations in the energy supply processes in the heart and liver, accompanied by oscillatory mechanisms of adaptation in the interval mode, causes a probable decrease in the production of urea and polyamines in plasma and liver, but not in erythrocytes. The administration of L-arginine prior to the IHT sessions increased the level of the nitrite-reducing component of the nitric oxide cycle, which persisted for up to 180 days of the experiment.

Conclusion: Thus, the efficacy of IHT and its nitrite-dependent component shown in this study is associated with the formation of long-term adaptive responses by preventing the intensification of lipoperoxidation processes in tissues due to pronounced changes in the main enzymes of antioxidant defence and stabilisation of erythrocyte membranes, which has a pronounced protective effect on the system of regulation of oxygen-dependent processes as a whole.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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