Weibiao Wang , Yuping Sa , Weiman Zhang , Xiaoying Wang , Yinli Wang , Hui Yuan , Liuyan Li , Shuqin Ding , Gidion Wilson , Xueqin Ma
{"title":"用离子交换树脂纯化和分离枸杞叶茶中的咖啡酰亚精胺衍生物及其作用机理","authors":"Weibiao Wang , Yuping Sa , Weiman Zhang , Xiaoying Wang , Yinli Wang , Hui Yuan , Liuyan Li , Shuqin Ding , Gidion Wilson , Xueqin Ma","doi":"10.1016/j.fbp.2024.06.005","DOIUrl":null,"url":null,"abstract":"<div><p>Goji leaf (<em>Lycium barbarum</em> leaves, LBL) tea is a well-known beverage that has been developed and utilized for its numerous health benefits. Recently, we have successfully extracted four caffeoyl spermidines derivatives from LBL (LBLS), namely N-caffeoylputrescine, N-acetyl-N′-caffeoylputrescine, N<sub>1</sub>-dihydrocaffeoyl-N<sub>10</sub>-caffeoylspermidine and N<sub>1</sub>, N<sub>10</sub>-dicaffeoylspermidine. Given the diverse bioactivities exhibited by LBLS, our study aimed to develop a precise separation method and explore the possible purification mechanism. Firstly, the extraction process was optimized, followed by the selection of 001×7 resin for the enrichment and purification of LBLS from six resins. Subsequently, the adsorption mechanism was comprehensively examined using FT-IR, DSC, XRD, and XPS techniques. Additionally, investigations into the adsorption kinetics, isotherm models, and adsorption thermodynamics revealed the adsorption process of LBLS on 001×7 resin was spontaneous and exothermic, followed a monolayer adsorption mechanism, and conformed to the pseudo-second-order kinetic model and Langmuir model. The optimal procedure involved adsorbing a 25 mg/mL LBL extract onto a 3.5 BV (bed volume) at 2 BV/h, then eluting with an 8 % NaCl-55 % ethanol solution for 5 BV at the same rate. Finally, LBLS was separated using PHPLC to obtain monomer compounds. This process yields 1.57 %±0.1 % LBLS, and four monomers with purity range from 90.7 % to 100 % were obtained.</p></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":"147 ","pages":"Pages 53-69"},"PeriodicalIF":3.5000,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Purification and separation of caffeoyl spermidine derivatives from goji leaf tea with ion exchange resin and the mechanisms involved\",\"authors\":\"Weibiao Wang , Yuping Sa , Weiman Zhang , Xiaoying Wang , Yinli Wang , Hui Yuan , Liuyan Li , Shuqin Ding , Gidion Wilson , Xueqin Ma\",\"doi\":\"10.1016/j.fbp.2024.06.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Goji leaf (<em>Lycium barbarum</em> leaves, LBL) tea is a well-known beverage that has been developed and utilized for its numerous health benefits. Recently, we have successfully extracted four caffeoyl spermidines derivatives from LBL (LBLS), namely N-caffeoylputrescine, N-acetyl-N′-caffeoylputrescine, N<sub>1</sub>-dihydrocaffeoyl-N<sub>10</sub>-caffeoylspermidine and N<sub>1</sub>, N<sub>10</sub>-dicaffeoylspermidine. Given the diverse bioactivities exhibited by LBLS, our study aimed to develop a precise separation method and explore the possible purification mechanism. Firstly, the extraction process was optimized, followed by the selection of 001×7 resin for the enrichment and purification of LBLS from six resins. Subsequently, the adsorption mechanism was comprehensively examined using FT-IR, DSC, XRD, and XPS techniques. Additionally, investigations into the adsorption kinetics, isotherm models, and adsorption thermodynamics revealed the adsorption process of LBLS on 001×7 resin was spontaneous and exothermic, followed a monolayer adsorption mechanism, and conformed to the pseudo-second-order kinetic model and Langmuir model. The optimal procedure involved adsorbing a 25 mg/mL LBL extract onto a 3.5 BV (bed volume) at 2 BV/h, then eluting with an 8 % NaCl-55 % ethanol solution for 5 BV at the same rate. Finally, LBLS was separated using PHPLC to obtain monomer compounds. This process yields 1.57 %±0.1 % LBLS, and four monomers with purity range from 90.7 % to 100 % were obtained.</p></div>\",\"PeriodicalId\":12134,\"journal\":{\"name\":\"Food and Bioproducts Processing\",\"volume\":\"147 \",\"pages\":\"Pages 53-69\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food and Bioproducts Processing\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0960308524001093\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioproducts Processing","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960308524001093","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Purification and separation of caffeoyl spermidine derivatives from goji leaf tea with ion exchange resin and the mechanisms involved
Goji leaf (Lycium barbarum leaves, LBL) tea is a well-known beverage that has been developed and utilized for its numerous health benefits. Recently, we have successfully extracted four caffeoyl spermidines derivatives from LBL (LBLS), namely N-caffeoylputrescine, N-acetyl-N′-caffeoylputrescine, N1-dihydrocaffeoyl-N10-caffeoylspermidine and N1, N10-dicaffeoylspermidine. Given the diverse bioactivities exhibited by LBLS, our study aimed to develop a precise separation method and explore the possible purification mechanism. Firstly, the extraction process was optimized, followed by the selection of 001×7 resin for the enrichment and purification of LBLS from six resins. Subsequently, the adsorption mechanism was comprehensively examined using FT-IR, DSC, XRD, and XPS techniques. Additionally, investigations into the adsorption kinetics, isotherm models, and adsorption thermodynamics revealed the adsorption process of LBLS on 001×7 resin was spontaneous and exothermic, followed a monolayer adsorption mechanism, and conformed to the pseudo-second-order kinetic model and Langmuir model. The optimal procedure involved adsorbing a 25 mg/mL LBL extract onto a 3.5 BV (bed volume) at 2 BV/h, then eluting with an 8 % NaCl-55 % ethanol solution for 5 BV at the same rate. Finally, LBLS was separated using PHPLC to obtain monomer compounds. This process yields 1.57 %±0.1 % LBLS, and four monomers with purity range from 90.7 % to 100 % were obtained.
期刊介绍:
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.