绿色植物中 CXE 基因家族的进化分化揭示了 PtoCXEs 过表达可减少转基因杨树的真菌定植。

IF 3.5 2区 农林科学 Q1 FORESTRY Tree physiology Pub Date : 2024-07-02 DOI:10.1093/treephys/tpae071
Dan Wang, Yuting Jin, Chaonan Guan, Qi Yang, Gang He, Nan Xu, Xuemin Han
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引用次数: 0

摘要

自植物登陆陆地以来,植物酶对迅速多样化的新陈代谢做出了重要贡献。羧基酯酶(CXE)是一种无处不在的多功能古老酶类,在植物进化过程中的多样性尤为突出。这项研究提供了病毒植物体内 CXE 的状况。研究人员从代表 9 个主要植物品系的 31 种植物基因组中鉴定出 784 个 CXE,并根据系统进化分析将其分为 5 个支系。支系 I 的 CXE 基因可能起源于细菌,然后在植物进化过程中扩展和多样化。第 II 支系最早出现在被植物移居陆地后的骨干植物祖先中,第 III 支系和第 IV 支系出现在被认为是最先进的无籽维管植物的蕨类植物中,而第 V 支系则出现在种子植物中。迄今为止,CXE 基因在木本植物中的功能仍不清楚。本研究从杨树基因组中鉴定出 51 个 CXE 基因,并进一步将其分为八类。串联复制和节段复制事件都促成了杨树中 CXE 基因的扩增。虽然 CXE 基因已被证明能增强许多草本植物对病原体的抵抗力,但对林木的相关研究仍有待进行。在这项研究中,病原体培养试验表明,在红豆杉中过表达六种第六类 CXE 或多或少地减少了真菌 Cytospora chrysosperma 在脱落叶片上的定殖。不同基因复制事件所产生的基因在功能分化模式上也存在明显差异。通过体内生理学分析,首次证实了杨树中重复的 CXE 基因的功能分化。潜在抗真菌 PtoCXE06 基因的鉴定也为促进林木抗病性状的遗传改良奠定了理论基础。
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Evolutionary divergence of CXE gene family in green plants unveils that PtoCXEs overexpression reduces fungal colonization in transgenic Populus.

Plant enzymes significantly contribute to the rapidly diversified metabolic repertoire since the colonization of land by plants. Carboxylesterase is just one of the ubiquitous, multifunctional and ancient enzymes that has particularly diversified during plant evolution. This study provided a status on the carboxylesterase landscape within Viridiplantae. A total of 784 carboxylesterases were identified from the genome of 31 plant species representing nine major lineages of sequenced Viridiplantae and divided into five clades based on phylogenetic analysis. Clade I carboxylesterase genes may be of bacterial origin and then expanded and diversified during plant evolution. Clade II was first gained in the ancestor of bryophytes after colonization of land by plants, Clade III and Clade IV in ferns which were considered the most advanced seedless vascular plants, while Clade V was gained in seed plants. To date, the functions of carboxylesterase genes in woody plants remain unclear. In this study, 51 carboxylesterase genes were identified from the genome of Populus trichocarpa and further divided into eight classes. Tandem and segmental duplication events both contributed to the expansion of carboxylesterase genes in Populus. Although carboxylesterase genes were proven to enhance resistance to pathogens in many herbaceous species, relevant researches on forest trees are still needed. In this study, pathogen incubation assays showed that overexpressing of six Class VI carboxylesterases in Populus tomentosa, to a greater or lesser degree, reduced colonization of detached leaves by fungus Cytospora chrysosperma. A significant difference was also found in functional divergence patterns for genes derived from different gene duplication events. Functional differentiation of duplicated carboxylesterase genes in Populus was proved for the first time by in vivo physiological analysis. The identification of the potentially anti-fungal PtoCXE06 gene also laid a theoretical foundation for promoting the genetic improvement of disease-resistance traits in forest trees.

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来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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