雾化对重组人源化 III 型胶原蛋白组成和结构的影响。

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241261904
Ningwen Cheng, Xinyue Zhang, Jian Wang, Danfeng Li, Ling Li, Huan Hu, Tingli Qu
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引用次数: 0

摘要

雾化是一种将吸入的液体制成气溶胶,并以雾或烟的形式输送到靶器官的治疗方法。它具有提高药物生物利用度、无痛、无创伤等优点,目前已广泛应用于肺部和口腔病变的治疗。气溶胶吸入作为治疗蛋白质的给药途径,因其能在非侵入性途径中实现高生物利用度而大有可为。目前,α-1 抗胰蛋白酶和 Dornase alfa 等大量治疗蛋白质都很有效。重组人源化 III 型胶原蛋白(rhCol III)作为一种治疗蛋白在生物医学领域得到了广泛应用,但雾化给药并不是 rhCol III 的常用给药途径,因此具有很大的发展潜力。然而,重组人源化胶原蛋白雾化后的结构稳定性有待进一步研究。本研究表明,通过压缩空气雾化的 rhCol III 保持了原有的分子量、三重螺旋结构和促进细胞粘附的能力。换句话说,rhCol III 在经过雾化后仍能保持其稳定性。虽然还需要更多的研究来确定雾化后 rhCol III 的有效性和安全性,但本研究可为今后的研究奠定基础。
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Effect of atomization on the composition and structure of recombinant humanized collagen type III.

Atomization is a treatment method to make inhaled liquids into aerosols and transport them to target organs in the form of fog or smoke. It has the advantages of improving the bioavailability of drugs, being painless, and non-invasive, and is now widely used in the treatment of lung and oral lesions. Aerosol inhalation as the route of administration of therapeutic proteins holds significant promise due to its ability to achieve high bioavailability in non-invasive pathways. Currently, a great number of therapeutic proteins such as alpha-1 antitrypsin and Dornase alfa are effective. Recombinant humanized collagen type III (rhCol III) as a therapeutic protein is widely used in the biomedical field, but atomization is not a common route of administration for rhCol III, presenting great potential for development. However, the structural stability of recombinant humanized collagen after atomization needs further investigation. This study demonstrated that the rhCol III subjected to atomization through compressed air had retained its original molecular weights, triple helical structures, and the ability to promote cell adhesion. In other words, the rhCol III can maintain its stability after undergoing atomization. Although more research is required to determine the efficacy and safety of the rhCol III after atomization, this study can lay the groundwork for future research.

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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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