在保卫细胞中过表达调质体 Ca2+-ATP 酶可协同增强气孔开放和耐旱性。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Integrative Plant Biology Pub Date : 2024-06-24 DOI:10.1111/jipb.13721
Jinghan Su, Bingqing He, Peiyuan Li, Baiyang Yu, Qiwen Cen, Lingfeng Xia, Yi Jing, Feibo Wu, Rucha Karnik, Dawei Xue, Michael R. Blatt, Yizhou Wang
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引用次数: 0

摘要

气孔通过控制水分状态和应对干旱胁迫在植物中发挥着至关重要的作用。然而,同时提高气孔开放度和耐旱性已被证明是一项重大挑战。为了解决这个问题,我们采用了 OnGuard 定量模型,该模型准确地反映了保卫细胞中离子转运体的力学和协调性。在 OnGuard 的指导下,我们成功地培育出了过量表达主要调质细胞 Ca2+-ATPase 基因 ACA11 的植物,该基因能促进气孔开放并增强植物生长。令人惊讶的是,这些转基因植物由于减少了通过气孔流失的水分,耐旱性也得到了提高。OnGuard 再次帮助我们了解了在过表达 ACA11 植物中观察到的意想不到的气孔行为背后的机制。我们的研究发现,ACA11 的过表达促进了 Ca2+ 在液泡中的积累,从而影响了 Ca2+ 的储存,并导致 Ca2+ 在赤霉酸作用下升高。这一调控级联精细调整了气孔反应,最终导致耐旱性增强。我们的发现强调了气孔Ca2+-ATP酶在操纵气孔行为和提高耐旱性方面的重要性。此外,这些结果还突显了定位于营养体的 ACA11 在应对不同条件时的多种功能,强调了其在未来植物强化中的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Overexpression of tonoplast Ca2+-ATPase in guard cells synergistically enhances stomatal opening and drought tolerance

Stomata play a crucial role in plants by controlling water status and responding to drought stress. However, simultaneously improving stomatal opening and drought tolerance has proven to be a significant challenge. To address this issue, we employed the OnGuard quantitative model, which accurately represents the mechanics and coordination of ion transporters in guard cells. With the guidance of OnGuard, we successfully engineered plants that overexpressed the main tonoplast Ca2+-ATPase gene, ACA11, which promotes stomatal opening and enhances plant growth. Surprisingly, these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their stomata. Again, OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the ACA11 overexpressing plants. Our study revealed that the overexpression of ACA11 facilitated the accumulation of Ca2+ in the vacuole, thereby influencing Ca2+ storage and leading to an enhanced Ca2+ elevation in response to abscisic acid. This regulatory cascade finely tunes stomatal responses, ultimately leading to enhanced drought tolerance. Our findings underscore the importance of tonoplast Ca2+-ATPase in manipulating stomatal behavior and improving drought tolerance. Furthermore, these results highlight the diverse functions of tonoplast-localized ACA11 in response to different conditions, emphasizing its potential for future applications in plant enhancement.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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