{"title":"开发用于水稻 G 到 T 编辑的鸟嘌呤碱基编辑器。","authors":"Lang Liu, Zhongming Zhang, Chenyang Wang, Fang Yan, Wenxian Sun, Xueping Zhou, Weiguo Miao, Huanbin Zhou","doi":"10.1111/jipb.13729","DOIUrl":null,"url":null,"abstract":"<p>Two guanine base editors created using an engineered N-methylpurine DNA glycosylase with CRISPR systems achieved targeted G-to-T editing with 4.94–12.50% efficiency in rice (<i>Oryza sativa</i>). The combined use of the DNA glycosylase and deaminases enabled co-editing of target guanines with adenines or cytosines.\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3000,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13729","citationCount":"0","resultStr":"{\"title\":\"Developing guanine base editors for G-to-T editing in rice\",\"authors\":\"Lang Liu, Zhongming Zhang, Chenyang Wang, Fang Yan, Wenxian Sun, Xueping Zhou, Weiguo Miao, Huanbin Zhou\",\"doi\":\"10.1111/jipb.13729\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Two guanine base editors created using an engineered N-methylpurine DNA glycosylase with CRISPR systems achieved targeted G-to-T editing with 4.94–12.50% efficiency in rice (<i>Oryza sativa</i>). The combined use of the DNA glycosylase and deaminases enabled co-editing of target guanines with adenines or cytosines.\\n <figure>\\n <div><picture>\\n <source></source></picture><p></p>\\n </div>\\n </figure></p>\",\"PeriodicalId\":195,\"journal\":{\"name\":\"Journal of Integrative Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.3000,\"publicationDate\":\"2024-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13729\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Integrative Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13729\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13729","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
利用工程化的 N-甲基嘌呤 DNA 糖基化酶和 CRISPR 系统创建的两个鸟嘌呤碱基编辑器在水稻（Oryza sativa）中实现了靶向 G 到 T 编辑，效率为 4.94-12.50%。DNA 糖基化酶和脱氨酶的联合使用实现了目标鸟嘌呤与腺嘌呤或胞嘧啶的共同编辑。
Developing guanine base editors for G-to-T editing in rice
Two guanine base editors created using an engineered N-methylpurine DNA glycosylase with CRISPR systems achieved targeted G-to-T editing with 4.94–12.50% efficiency in rice (Oryza sativa). The combined use of the DNA glycosylase and deaminases enabled co-editing of target guanines with adenines or cytosines.
期刊介绍：
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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