Qi Pan, Shi-Jiang Yu, Shuang Lei, Shao-Hui Zhang, Li-Li Ding, Liu Liu, Si-Chen Li, Xue-Feng Wang, Bing-Hai Lou* and Chun Ran*,
{"title":"细菌共生体通过改变宿主解毒基因的表达水平提高柑橘拟尾柱虫对杀虫剂的敏感性","authors":"Qi Pan, Shi-Jiang Yu, Shuang Lei, Shao-Hui Zhang, Li-Li Ding, Liu Liu, Si-Chen Li, Xue-Feng Wang, Bing-Hai Lou* and Chun Ran*, ","doi":"10.1021/acs.jafc.4c03049","DOIUrl":null,"url":null,"abstract":"<p >Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate the relationship between the structure of gut bacterial symbionts and insecticide susceptibility in <i>Diaphorina citri</i>, the important carrier of <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las), the causal agent of Huanglongbing (HLB). Our results indicated that antibiotic treatment significantly increased the susceptibility of <i>D. citri</i> to bifenthrin and thiamethoxam, and significantly decreased the relative abundance of <i>Wolbachia</i> and Profftella, enzyme activities of CarEs, and expression level of multiple CarE genes. The relative loads of <i>Wolbachia</i> and Profftella were positively correlated with <i>DcitCCE13</i>, <i>DcitCCE14</i>, <i>DcitCCE15</i>, and <i>DcitCCE16</i>. RNAi and prokaryotic expression revealed that <i>DcitCCE15</i> is associated with bifenthrin metabolism. These results revealed that bacterial symbionts might regulate <i>DcitCCE15</i> expression, which is involved in the susceptibility of <i>D. citri</i> to bifenthrin.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacterial Symbionts Contribute to Insecticide Susceptibility of Diaphorina citri via Changing the Expression Level of Host Detoxifying Genes\",\"authors\":\"Qi Pan, Shi-Jiang Yu, Shuang Lei, Shao-Hui Zhang, Li-Li Ding, Liu Liu, Si-Chen Li, Xue-Feng Wang, Bing-Hai Lou* and Chun Ran*, \",\"doi\":\"10.1021/acs.jafc.4c03049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate the relationship between the structure of gut bacterial symbionts and insecticide susceptibility in <i>Diaphorina citri</i>, the important carrier of <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las), the causal agent of Huanglongbing (HLB). Our results indicated that antibiotic treatment significantly increased the susceptibility of <i>D. citri</i> to bifenthrin and thiamethoxam, and significantly decreased the relative abundance of <i>Wolbachia</i> and Profftella, enzyme activities of CarEs, and expression level of multiple CarE genes. The relative loads of <i>Wolbachia</i> and Profftella were positively correlated with <i>DcitCCE13</i>, <i>DcitCCE14</i>, <i>DcitCCE15</i>, and <i>DcitCCE16</i>. RNAi and prokaryotic expression revealed that <i>DcitCCE15</i> is associated with bifenthrin metabolism. These results revealed that bacterial symbionts might regulate <i>DcitCCE15</i> expression, which is involved in the susceptibility of <i>D. citri</i> to bifenthrin.</p>\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jafc.4c03049\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.4c03049","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Bacterial Symbionts Contribute to Insecticide Susceptibility of Diaphorina citri via Changing the Expression Level of Host Detoxifying Genes
Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate the relationship between the structure of gut bacterial symbionts and insecticide susceptibility in Diaphorina citri, the important carrier of Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing (HLB). Our results indicated that antibiotic treatment significantly increased the susceptibility of D. citri to bifenthrin and thiamethoxam, and significantly decreased the relative abundance of Wolbachia and Profftella, enzyme activities of CarEs, and expression level of multiple CarE genes. The relative loads of Wolbachia and Profftella were positively correlated with DcitCCE13, DcitCCE14, DcitCCE15, and DcitCCE16. RNAi and prokaryotic expression revealed that DcitCCE15 is associated with bifenthrin metabolism. These results revealed that bacterial symbionts might regulate DcitCCE15 expression, which is involved in the susceptibility of D. citri to bifenthrin.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.