Ignacio Fernández-Pastor*, Victor González-Menéndez, Ignacio González, Rachel Serrano, Thomas A. Mackenzie, Guillermo Benítez, Manuel Casares-Porcel, Olga Genilloud and Fernando Reyes*,
{"title":"Escuzarmycins A-D,控制三尖杉斑病的强效生物杀真菌剂","authors":"Ignacio Fernández-Pastor*, Victor González-Menéndez, Ignacio González, Rachel Serrano, Thomas A. Mackenzie, Guillermo Benítez, Manuel Casares-Porcel, Olga Genilloud and Fernando Reyes*, ","doi":"10.1021/acs.jafc.4c01303","DOIUrl":null,"url":null,"abstract":"<p >A study targeting novel antifungal metabolites identified potent <i>in vitro</i> antifungal activity against key plant pathogens in acetone extracts of <i>Streptomyces</i> sp. strain CA-296093. Feature-based molecular networking revealed the presence in this extract of antimycin-related compounds, leading to the isolation of four new compounds: escuzarmycins A–D (<b>1</b>–<b>4</b>). Extensive structural elucidation, employing 1D and 2D NMR, high-resolution mass spectrometry, Marfey’s analysis, and NOESY correlations, confirmed their structures. The bioactivity of these compounds was tested against six fungal phytopathogens, and compounds <b>3</b> and <b>4</b> demonstrated strong efficacy, particularly against <i>Zymoseptoria tritici</i>, with compound <b>3</b> exhibiting the highest potency (EC<sub>50</sub>: 11 nM). Both compounds also displayed significant antifungal activity against <i>Botrytis cinerea</i> and <i>Colletotrichum acutatum</i>, with compound <b>4</b> proving to be the most potent. Despite moderate cytotoxicity against the human cancer cell line HepG2, compounds <b>3</b> and <b>4</b> emerge as promising fungicides for combating <i>Septoria tritici</i> blotch, anthracnose, and gray mold.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Escuzarmycins A–D, Potent Biofungicides to Control Septoria tritici Blotch\",\"authors\":\"Ignacio Fernández-Pastor*, Victor González-Menéndez, Ignacio González, Rachel Serrano, Thomas A. Mackenzie, Guillermo Benítez, Manuel Casares-Porcel, Olga Genilloud and Fernando Reyes*, \",\"doi\":\"10.1021/acs.jafc.4c01303\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >A study targeting novel antifungal metabolites identified potent <i>in vitro</i> antifungal activity against key plant pathogens in acetone extracts of <i>Streptomyces</i> sp. strain CA-296093. Feature-based molecular networking revealed the presence in this extract of antimycin-related compounds, leading to the isolation of four new compounds: escuzarmycins A–D (<b>1</b>–<b>4</b>). Extensive structural elucidation, employing 1D and 2D NMR, high-resolution mass spectrometry, Marfey’s analysis, and NOESY correlations, confirmed their structures. The bioactivity of these compounds was tested against six fungal phytopathogens, and compounds <b>3</b> and <b>4</b> demonstrated strong efficacy, particularly against <i>Zymoseptoria tritici</i>, with compound <b>3</b> exhibiting the highest potency (EC<sub>50</sub>: 11 nM). Both compounds also displayed significant antifungal activity against <i>Botrytis cinerea</i> and <i>Colletotrichum acutatum</i>, with compound <b>4</b> proving to be the most potent. Despite moderate cytotoxicity against the human cancer cell line HepG2, compounds <b>3</b> and <b>4</b> emerge as promising fungicides for combating <i>Septoria tritici</i> blotch, anthracnose, and gray mold.</p>\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jafc.4c01303\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.4c01303","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Escuzarmycins A–D, Potent Biofungicides to Control Septoria tritici Blotch
A study targeting novel antifungal metabolites identified potent in vitro antifungal activity against key plant pathogens in acetone extracts of Streptomyces sp. strain CA-296093. Feature-based molecular networking revealed the presence in this extract of antimycin-related compounds, leading to the isolation of four new compounds: escuzarmycins A–D (1–4). Extensive structural elucidation, employing 1D and 2D NMR, high-resolution mass spectrometry, Marfey’s analysis, and NOESY correlations, confirmed their structures. The bioactivity of these compounds was tested against six fungal phytopathogens, and compounds 3 and 4 demonstrated strong efficacy, particularly against Zymoseptoria tritici, with compound 3 exhibiting the highest potency (EC50: 11 nM). Both compounds also displayed significant antifungal activity against Botrytis cinerea and Colletotrichum acutatum, with compound 4 proving to be the most potent. Despite moderate cytotoxicity against the human cancer cell line HepG2, compounds 3 and 4 emerge as promising fungicides for combating Septoria tritici blotch, anthracnose, and gray mold.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.