银桦蔗糖分化过程中 CWINV 和 SUS 基因对蔗糖利用的影响

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Protein and Peptide Letters Pub Date : 2024-01-01 DOI:10.2174/0109298665309207240621094227
Yulia Leonidovna Moshchenskaya, Natalia Alekseevna Galibina, Aleksandra Aleksandrovna Serkova, Tatyana Vladimirovna Tarelkina, Ksenia Michailovna Nikerova, Maksim Anatol'evich Korzhenevsky, Irina Nikolaevna Sofronova, Ludmila Igorevna Semenova
{"title":"银桦蔗糖分化过程中 CWINV 和 SUS 基因对蔗糖利用的影响","authors":"Yulia Leonidovna Moshchenskaya, Natalia Alekseevna Galibina, Aleksandra Aleksandrovna Serkova, Tatyana Vladimirovna Tarelkina, Ksenia Michailovna Nikerova, Maksim Anatol'evich Korzhenevsky, Irina Nikolaevna Sofronova, Ludmila Igorevna Semenova","doi":"10.2174/0109298665309207240621094227","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The mechanisms that control the accumulation of woody biomass are of great interest to the study. Invertase and sucrose synthase are enzymes that are vital for distributing carbon in various biosynthetic pathways. Karelian birch (<i>Betula pendula</i> var. <i>carelica</i>) is a form of silver birch (<i>B. pendula</i> Roth) and is characterized by disruption of the differentiation of cambium derivatives towards both the xylem and phloem, which leads to a change in the proportion of the conducting tissues' structural elements and the figured wood formation. We researched the expression profiles of genes encoding sucrose-cleaving enzymes (<i>CWINV</i> and <i>SUS</i> gene families) and genes encoding CVIF protein, which is responsible for the post-translational regulation of the cell wall invertase activity.</p><p><strong>Objective: </strong>In our study, 16-year-old common silver birch (<i>Betula pendula</i> var. <i>pendula</i>) and Karelian birch were used for sampling non-figured and figured trunk section tissues, respectively. Samples were selected for the research based on the radial vector: non-conductive, conductive phloem, cambial zone - differentiating xylem - mature xylem.</p><p><strong>Methods: </strong>The enzyme's activity was investigated by biochemical methods. RT-PCR method was used to determine the level of gene expression. Anatomical and morphological methods were used to determine the stage of differentiation of xylem cambial derivatives.</p><p><strong>Results: </strong>Our research revealed a shift in the composition of xylem components in figured Karelian birch, characterized by increased parenchymatization and reduced vessel quantity. In all studied trunk tissues of Karelian birch, compared with common silver birch, an increase in the expression of the <i>CWINV</i> gene family and the <i>SUS3</i> gene and a decrease in the expression of SUS4 were shown.</p><p><strong>Conclusion: </strong>Therefore, the increase in parenchymatization in figured Karelian birch is linked to a shift in sucrose metabolism towards the apoplastic pathway, indicated by a higher cell wall invertase activity and gene expression. The expression of the <i>SUS4</i> gene correlates with the decrease in xylem increments and vessel proportion. The research findings will enhance our understanding of how sucrose breaking enzymes regulate secondary growth in woody plants and aid in developing practical timber cultivation methods.</p>","PeriodicalId":20736,"journal":{"name":"Protein and Peptide Letters","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Participation of <i>CWINV</i> and <i>SUS</i> Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch.\",\"authors\":\"Yulia Leonidovna Moshchenskaya, Natalia Alekseevna Galibina, Aleksandra Aleksandrovna Serkova, Tatyana Vladimirovna Tarelkina, Ksenia Michailovna Nikerova, Maksim Anatol'evich Korzhenevsky, Irina Nikolaevna Sofronova, Ludmila Igorevna Semenova\",\"doi\":\"10.2174/0109298665309207240621094227\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The mechanisms that control the accumulation of woody biomass are of great interest to the study. Invertase and sucrose synthase are enzymes that are vital for distributing carbon in various biosynthetic pathways. Karelian birch (<i>Betula pendula</i> var. <i>carelica</i>) is a form of silver birch (<i>B. pendula</i> Roth) and is characterized by disruption of the differentiation of cambium derivatives towards both the xylem and phloem, which leads to a change in the proportion of the conducting tissues' structural elements and the figured wood formation. We researched the expression profiles of genes encoding sucrose-cleaving enzymes (<i>CWINV</i> and <i>SUS</i> gene families) and genes encoding CVIF protein, which is responsible for the post-translational regulation of the cell wall invertase activity.</p><p><strong>Objective: </strong>In our study, 16-year-old common silver birch (<i>Betula pendula</i> var. <i>pendula</i>) and Karelian birch were used for sampling non-figured and figured trunk section tissues, respectively. Samples were selected for the research based on the radial vector: non-conductive, conductive phloem, cambial zone - differentiating xylem - mature xylem.</p><p><strong>Methods: </strong>The enzyme's activity was investigated by biochemical methods. RT-PCR method was used to determine the level of gene expression. Anatomical and morphological methods were used to determine the stage of differentiation of xylem cambial derivatives.</p><p><strong>Results: </strong>Our research revealed a shift in the composition of xylem components in figured Karelian birch, characterized by increased parenchymatization and reduced vessel quantity. In all studied trunk tissues of Karelian birch, compared with common silver birch, an increase in the expression of the <i>CWINV</i> gene family and the <i>SUS3</i> gene and a decrease in the expression of SUS4 were shown.</p><p><strong>Conclusion: </strong>Therefore, the increase in parenchymatization in figured Karelian birch is linked to a shift in sucrose metabolism towards the apoplastic pathway, indicated by a higher cell wall invertase activity and gene expression. The expression of the <i>SUS4</i> gene correlates with the decrease in xylem increments and vessel proportion. The research findings will enhance our understanding of how sucrose breaking enzymes regulate secondary growth in woody plants and aid in developing practical timber cultivation methods.</p>\",\"PeriodicalId\":20736,\"journal\":{\"name\":\"Protein and Peptide Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Protein and Peptide Letters\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.2174/0109298665309207240621094227\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protein and Peptide Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2174/0109298665309207240621094227","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

背景:控制木本生物量积累的机制是研究的重点。转化酶和蔗糖合成酶是在各种生物合成途径中分配碳的重要酶。卡累利阿桦树(Betula pendula var. carelica)是银桦树(B. pendula Roth)的一种,其特点是骨皮层衍生物向木质部和韧皮部的分化受到破坏,从而导致传导组织结构元素的比例发生变化,并形成琢磨不透的木质部。我们研究了编码蔗糖分解酶(CWINV 和 SUS 基因家族)的基因和编码 CVIF 蛋白的基因的表达谱,CVIF 蛋白负责细胞壁转化酶活性的翻译后调控:在我们的研究中,16 岁的普通银桦(Betula pendula var.研究样本的选择以径向载体为基础:非导电韧皮部、导电韧皮部、结皮区--分化木质部--成熟木质部:方法:采用生化方法研究酶的活性。采用 RT-PCR 方法测定基因表达水平。采用解剖学和形态学方法确定木质部分化衍生物的阶段:结果:我们的研究发现,卡累利阿桦木木质部成分发生了变化,其特点是实质化程度提高,血管数量减少。在所有研究的卡累利阿桦树树干组织中,与普通银桦相比,CWINV 基因家族和 SUS3 基因的表达量增加,SUS4 基因的表达量减少:因此,卡累利阿桦实肉化程度的提高与蔗糖代谢转向细胞外途径有关,细胞壁转化酶活性和基因表达量的提高表明了这一点。SUS4 基因的表达与木质部增量和血管比例的减少有关。研究结果将加深我们对蔗糖分解酶如何调控木本植物次生生长的理解,并有助于开发实用的木材栽培方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Participation of CWINV and SUS Genes in Sucrose Utilization in the Disruption of Cambium Derivatives Differentiation of Silver Birch.

Background: The mechanisms that control the accumulation of woody biomass are of great interest to the study. Invertase and sucrose synthase are enzymes that are vital for distributing carbon in various biosynthetic pathways. Karelian birch (Betula pendula var. carelica) is a form of silver birch (B. pendula Roth) and is characterized by disruption of the differentiation of cambium derivatives towards both the xylem and phloem, which leads to a change in the proportion of the conducting tissues' structural elements and the figured wood formation. We researched the expression profiles of genes encoding sucrose-cleaving enzymes (CWINV and SUS gene families) and genes encoding CVIF protein, which is responsible for the post-translational regulation of the cell wall invertase activity.

Objective: In our study, 16-year-old common silver birch (Betula pendula var. pendula) and Karelian birch were used for sampling non-figured and figured trunk section tissues, respectively. Samples were selected for the research based on the radial vector: non-conductive, conductive phloem, cambial zone - differentiating xylem - mature xylem.

Methods: The enzyme's activity was investigated by biochemical methods. RT-PCR method was used to determine the level of gene expression. Anatomical and morphological methods were used to determine the stage of differentiation of xylem cambial derivatives.

Results: Our research revealed a shift in the composition of xylem components in figured Karelian birch, characterized by increased parenchymatization and reduced vessel quantity. In all studied trunk tissues of Karelian birch, compared with common silver birch, an increase in the expression of the CWINV gene family and the SUS3 gene and a decrease in the expression of SUS4 were shown.

Conclusion: Therefore, the increase in parenchymatization in figured Karelian birch is linked to a shift in sucrose metabolism towards the apoplastic pathway, indicated by a higher cell wall invertase activity and gene expression. The expression of the SUS4 gene correlates with the decrease in xylem increments and vessel proportion. The research findings will enhance our understanding of how sucrose breaking enzymes regulate secondary growth in woody plants and aid in developing practical timber cultivation methods.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Protein and Peptide Letters
Protein and Peptide Letters 生物-生化与分子生物学
CiteScore
2.90
自引率
0.00%
发文量
98
审稿时长
2 months
期刊介绍: Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis
期刊最新文献
Immunoproteomics: A Review of Techniques, Applications, and Advancements. SGSM2 in Uveal Melanoma: Implications for Survival, Immune Infiltration, and Drug Sensitivity. Exploring the Regulatory Interaction of Differentially Expressed Proteins in Cleft Palate Induced by Retinoic Acid. Immunoproteomics: Approach to Diagnostic and Vaccine Development. Characterization of Luciferase from Photorhabdus kayaii and its Application for In vivo Imaging Studies in Mice.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1