抑制多胺平衡可通过调节盐胁迫向日葵幼苗中 IAA 和 PIN1 的分布促进根系伸展

IF 2.2 4区 生物学 Q2 PLANT SCIENCES Theoretical and Experimental Plant Physiology Pub Date : 2024-06-24 DOI:10.1007/s40626-024-00342-3
Aditi Tailor, Satish C. Bhatla
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引用次数: 0

摘要

植物根系的生长和发育会发生重大变化,以适应环境胁迫条件。暴露于盐度的根的重塑是由涉及植物激素、一氧化氮(NO)和活性氧(ROS)的各种信号通路之间复杂的相互作用协调的。多胺(PAs)是一种阳离子小胺分子,在植物生长和胁迫反应中具有多种作用,也被认为会影响根的形态。研究表明,用 PA 生物合成抑制剂[DFMA(DL-α-二氟甲基精氨酸)或 DFMO(DL-α-二氟甲基鸟氨酸)]处理向日葵幼苗,可促进主根在无 NaCl 或有 NaCl 的情况下伸展生长。本研究探讨了 PAs 及其与辅助素信号传导在向日葵幼苗根系形态建模中可能发挥的作用。我们观察到,盐度对根生长的抑制可能是由 PIN1(辅素外流蛋白)极性定位的破坏导致的根顶端 IAA 缺乏。与此相反,施用 PA 抑制剂(DFMA 或 DFMO)后,相对于对照组(-NaCl),幼苗根尖的 IAA 积累增加,尽管同时暴露于 120 毫摩尔 NaCl 的幼苗增加的程度较小。IAA 积累的这些变化与之前报道的向日葵幼苗在无 NaCl 或有 NaCl 的情况下对 PA 抑制剂处理的主根伸展变化相吻合。我们发现,在接受 PA 生物合成抑制剂和 NaCl 联合处理的幼苗中观察到的根系伸展的增强可能涉及 PIN1 在根细胞中极性分布的维持,这反过来又可能是恢复 IAA 在根顶分布的原因,从而进一步支持观察到的主根的伸展生长。另一方面,一氧化氮和过氧化氢在所观察到的 PA 抑制剂触发的根延伸反应中的作用目前仍不确定。因此,在向日葵幼苗暴露于盐度条件下的根系结构重塑过程中,PAs 及其与 auxin 之间的串扰作用是显而易见的。
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Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings

Plant root growth and development undergo significant alterations as an adaptation to environmental stressful conditions. Remodeling of roots exposed to salinity is coordinated by complex interactions among various signaling pathways involving phytohormones, nitric oxide (NO), and reactive oxygen species (ROS). Polyamines (PAs), small, cationic amine molecules with diverse roles in plant growth and stress responses, are also known to influence root morphology. Studies reported that treatment of sunflower seedlings with PA biosynthesis inhibitors [DFMA (DL-α-difluoromethylarginine) or DFMO (DL-α-difluoromethylornithine)], promotes extension growth of primary roots both in the absence or presence of NaCl. This work explores the possible role of PAs and their crosstalk with auxin signaling in the modeling of the root morphology in etiolated sunflower seedlings. We observed that inhibition of root growth by salinity is possibly governed by a disruption of the polar localization of PIN1 (auxin efflux protein) leading to IAA deficiency in the root apex. Application of PA inhibitors (DFMA or DFMO), in contrast, brings about an enhancement in IAA accumulation in the root apices of seedlings relative to control (−NaCl), albeit to a lesser degree in seedlings also exposed simultaneously to 120 mM NaCl. These alterations in IAA accumulation coincide with changes in primary root extension previously reported in sunflower seedlings in response to treatment with PA inhibitors, both in the absence or presence of NaCl. We found that the enhancement in root extension observed in seedlings subjected to a combined treatment of PA biosynthesis inhibitors and NaCl possibly involves the maintenance of polar distribution of PIN1 in the root cells which, in turn, may be responsible for the restoration of IAA distribution in the root apex to further support the observed extension growth of primary root. On the other hand, the role of nitric oxide and hydrogen peroxide in the observed PA inhibitor-triggered response on root extension remains uncertain at present. Therefore, a possible role of PAs and their crosstalk with auxin is evident in root architecture remodeling in sunflower seedlings exposed to salinity.

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来源期刊
CiteScore
4.20
自引率
7.70%
发文量
32
期刊介绍: The journal does not publish articles in taxonomy, anatomy, systematics and ecology unless they have a physiological approach related to the following sections: Biochemical Processes: primary and secondary metabolism, and biochemistry; Photobiology and Photosynthesis Processes; Cell Biology; Genes and Development; Plant Molecular Biology; Signaling and Response; Plant Nutrition; Growth and Differentiation: seed physiology, hormonal physiology and photomorphogenesis; Post-Harvest Physiology; Ecophysiology/Crop Physiology and Stress Physiology; Applied Plant Ecology; Plant-Microbe and Plant-Insect Interactions; Instrumentation in Plant Physiology; Education in Plant Physiology.
期刊最新文献
Reactive oxygen species in pollination drops of coniferous plants Ammonium induces aquaporin gene expression in Guzmania monostachia (Bromeliaceae) under drought The nematophagous root endophyte Pochonia chlamydosporia enhances tolerance to drought in soybean The expression of 1-Cys-PRX reflects changes in Coffea arabica seed quality during storage Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings
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