用于测定盐酸哌唑嗪的紫外分光光度法的开发与验证

IF 0.8 4区 化学 Q4 SPECTROSCOPY Journal of Applied Spectroscopy Pub Date : 2024-07-04 DOI:10.1007/s10812-024-01771-3
Chodimella Sahitya Bharathi, Raja Sundararajan
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引用次数: 0

摘要

本研究旨在建立一种新型、快速、准确的紫外分光光度法,用于定量检测纯品和片剂中的盐酸哌唑嗪。采用 pH 值为 6 的磷酸盐缓冲溶液作为稀释剂。盐酸哌唑嗪的最高吸光度为 247 nm,线性范围为 2 至 8 μg/mL。盐酸哌唑嗪的回归方程为 y= 0.087x + 0.236,相关系数为 0.987。回收率为 99.6%至 101%。日内精密度和日间精密度的相对标准偏差均小于 2。盐酸哌唑嗪的最低检测限和最低定量限分别为 0.0375 和 0.113 μg/mL。国际协调委员会的标准验证了该光谱分析技术,适用于纯品和片剂盐酸哌唑嗪的常规定量测定。
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Development and Validation of UV Spectrophotometric Method for Determination of Prazosin Hydrochloride

This work was aimed at creating a new, fast, and accurate UV spectrophotometric method for quantifying prazosin hydrochloride in pure and tablet dosage forms. A phosphate buffer solution of pH 6 was used as a diluent. The highest absorbance of prazosin hydrochloride was measured at 247 nm, and the linearity ranged from 2 to 8 μg/mL. The regression equation for prazosin hydrochloride was y= 0.087x + 0.236, with a correlation value 0.987. The percentage of recovery ranged from 99.6 to 101%. The relative standard deviation for intraday precision and interday precision was determined to be less than 2. The LOD and LOQ of prazosin hydrochloride were determined to be 0.0375 and 0.113 μg/mL, respectively. International Council for Harmonisation criteria validated the spectrometric technique and was suitable for routine quantitative measurement of prazosin hydrochloride in pure and tablet dosage forms.

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来源期刊
CiteScore
1.30
自引率
14.30%
发文量
145
审稿时长
2.5 months
期刊介绍: Journal of Applied Spectroscopy reports on many key applications of spectroscopy in chemistry, physics, metallurgy, and biology. An increasing number of papers focus on the theory of lasers, as well as the tremendous potential for the practical applications of lasers in numerous fields and industries.
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