Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Gulnora Mirzaeva, Bakhtiyor Kholmatov, Erinda Lika
{"title":"对反刍动物中蜱虫动态和无形体病的见解:微观和分子视角","authors":"Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Gulnora Mirzaeva, Bakhtiyor Kholmatov, Erinda Lika","doi":"10.1111/azo.12510","DOIUrl":null,"url":null,"abstract":"Ticks are blood‐sucking parasite and transmit pathogens to humans, domestic and wild animals. Ticks are seriously damage the production of sheep and goats, resulting in heavy economic loss to farmers. The present study investigated microscopic and molecular identification of ticks and tick‐borne anaplasmosis infesting sheep and goats in three agro‐ecological zones of Pakistan. The collected ticks were identified at species level based on their morphological features through microscopy by using taxonomic keys. Four <jats:italic>Rhipicephalus (R.)</jats:italic> species namely: <jats:italic>R. guilhoni Rh. geigyi</jats:italic>, <jats:italic>R. annulatus</jats:italic>, <jats:italic>R. pulchellus</jats:italic>, two <jats:italic>Haemaphysalis (Hae)</jats:italic> spp. <jats:italic>Hae. sulcate</jats:italic>, <jats:italic>Hae. punctate</jats:italic>, one <jats:italic>Ixodes ricinus and Hyalomma anatolicum</jats:italic> were identified. The morphological variations in their body sizes, scutal pattern, female genital opening, spiracular plate length and male adanal plate were recorded. Furthermore, we assessed the effectiveness of utilizing the 16S rRNA gene sequence to differentiate <jats:italic>Anaplasma</jats:italic> samples at the species level. Microscopically suspected positive blood samples for <jats:italic>Anaplasma</jats:italic> were subjected to DNA extraction. <jats:italic>Anaplasma</jats:italic> was observed in the blood of 14 animals. The sequence similarity between <jats:italic>Anaplasma marginale</jats:italic> and the reference genome were 99.25%, while below this value indicating a distinct species. Phylogenetic analysis revealed close similarity with <jats:italic>A. marginale</jats:italic> isolates from Iran, Vietnam and Pakistan This study contributes to the epidemiology of ticks and TBPs in the KP province and highlights the importance of proactive TBP surveillance in livestock.","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Insights into tick dynamics and anaplasmosis in ruminants: A microscopic and molecular perspective\",\"authors\":\"Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Gulnora Mirzaeva, Bakhtiyor Kholmatov, Erinda Lika\",\"doi\":\"10.1111/azo.12510\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ticks are blood‐sucking parasite and transmit pathogens to humans, domestic and wild animals. Ticks are seriously damage the production of sheep and goats, resulting in heavy economic loss to farmers. The present study investigated microscopic and molecular identification of ticks and tick‐borne anaplasmosis infesting sheep and goats in three agro‐ecological zones of Pakistan. The collected ticks were identified at species level based on their morphological features through microscopy by using taxonomic keys. Four <jats:italic>Rhipicephalus (R.)</jats:italic> species namely: <jats:italic>R. guilhoni Rh. geigyi</jats:italic>, <jats:italic>R. annulatus</jats:italic>, <jats:italic>R. pulchellus</jats:italic>, two <jats:italic>Haemaphysalis (Hae)</jats:italic> spp. <jats:italic>Hae. sulcate</jats:italic>, <jats:italic>Hae. punctate</jats:italic>, one <jats:italic>Ixodes ricinus and Hyalomma anatolicum</jats:italic> were identified. The morphological variations in their body sizes, scutal pattern, female genital opening, spiracular plate length and male adanal plate were recorded. Furthermore, we assessed the effectiveness of utilizing the 16S rRNA gene sequence to differentiate <jats:italic>Anaplasma</jats:italic> samples at the species level. Microscopically suspected positive blood samples for <jats:italic>Anaplasma</jats:italic> were subjected to DNA extraction. <jats:italic>Anaplasma</jats:italic> was observed in the blood of 14 animals. The sequence similarity between <jats:italic>Anaplasma marginale</jats:italic> and the reference genome were 99.25%, while below this value indicating a distinct species. Phylogenetic analysis revealed close similarity with <jats:italic>A. marginale</jats:italic> isolates from Iran, Vietnam and Pakistan This study contributes to the epidemiology of ticks and TBPs in the KP province and highlights the importance of proactive TBP surveillance in livestock.\",\"PeriodicalId\":50945,\"journal\":{\"name\":\"Acta Zoologica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Zoologica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/azo.12510\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Zoologica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/azo.12510","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
Insights into tick dynamics and anaplasmosis in ruminants: A microscopic and molecular perspective
Ticks are blood‐sucking parasite and transmit pathogens to humans, domestic and wild animals. Ticks are seriously damage the production of sheep and goats, resulting in heavy economic loss to farmers. The present study investigated microscopic and molecular identification of ticks and tick‐borne anaplasmosis infesting sheep and goats in three agro‐ecological zones of Pakistan. The collected ticks were identified at species level based on their morphological features through microscopy by using taxonomic keys. Four Rhipicephalus (R.) species namely: R. guilhoni Rh. geigyi, R. annulatus, R. pulchellus, two Haemaphysalis (Hae) spp. Hae. sulcate, Hae. punctate, one Ixodes ricinus and Hyalomma anatolicum were identified. The morphological variations in their body sizes, scutal pattern, female genital opening, spiracular plate length and male adanal plate were recorded. Furthermore, we assessed the effectiveness of utilizing the 16S rRNA gene sequence to differentiate Anaplasma samples at the species level. Microscopically suspected positive blood samples for Anaplasma were subjected to DNA extraction. Anaplasma was observed in the blood of 14 animals. The sequence similarity between Anaplasma marginale and the reference genome were 99.25%, while below this value indicating a distinct species. Phylogenetic analysis revealed close similarity with A. marginale isolates from Iran, Vietnam and Pakistan This study contributes to the epidemiology of ticks and TBPs in the KP province and highlights the importance of proactive TBP surveillance in livestock.
期刊介绍:
Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.