对反刍动物中蜱虫动态和无形体病的见解:微观和分子视角

IF 1.1 4区 生物学 Q4 ANATOMY & MORPHOLOGY Acta Zoologica Pub Date : 2024-07-03 DOI:10.1111/azo.12510
Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Gulnora Mirzaeva, Bakhtiyor Kholmatov, Erinda Lika
{"title":"对反刍动物中蜱虫动态和无形体病的见解:微观和分子视角","authors":"Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Gulnora Mirzaeva, Bakhtiyor Kholmatov, Erinda Lika","doi":"10.1111/azo.12510","DOIUrl":null,"url":null,"abstract":"Ticks are blood‐sucking parasite and transmit pathogens to humans, domestic and wild animals. Ticks are seriously damage the production of sheep and goats, resulting in heavy economic loss to farmers. The present study investigated microscopic and molecular identification of ticks and tick‐borne anaplasmosis infesting sheep and goats in three agro‐ecological zones of Pakistan. The collected ticks were identified at species level based on their morphological features through microscopy by using taxonomic keys. Four <jats:italic>Rhipicephalus (R.)</jats:italic> species namely: <jats:italic>R. guilhoni Rh. geigyi</jats:italic>, <jats:italic>R. annulatus</jats:italic>, <jats:italic>R. pulchellus</jats:italic>, two <jats:italic>Haemaphysalis (Hae)</jats:italic> spp. <jats:italic>Hae. sulcate</jats:italic>, <jats:italic>Hae. punctate</jats:italic>, one <jats:italic>Ixodes ricinus and Hyalomma anatolicum</jats:italic> were identified. The morphological variations in their body sizes, scutal pattern, female genital opening, spiracular plate length and male adanal plate were recorded. Furthermore, we assessed the effectiveness of utilizing the 16S rRNA gene sequence to differentiate <jats:italic>Anaplasma</jats:italic> samples at the species level. Microscopically suspected positive blood samples for <jats:italic>Anaplasma</jats:italic> were subjected to DNA extraction. <jats:italic>Anaplasma</jats:italic> was observed in the blood of 14 animals. The sequence similarity between <jats:italic>Anaplasma marginale</jats:italic> and the reference genome were 99.25%, while below this value indicating a distinct species. Phylogenetic analysis revealed close similarity with <jats:italic>A. marginale</jats:italic> isolates from Iran, Vietnam and Pakistan This study contributes to the epidemiology of ticks and TBPs in the KP province and highlights the importance of proactive TBP surveillance in livestock.","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":"16 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Insights into tick dynamics and anaplasmosis in ruminants: A microscopic and molecular perspective\",\"authors\":\"Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Gulnora Mirzaeva, Bakhtiyor Kholmatov, Erinda Lika\",\"doi\":\"10.1111/azo.12510\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ticks are blood‐sucking parasite and transmit pathogens to humans, domestic and wild animals. Ticks are seriously damage the production of sheep and goats, resulting in heavy economic loss to farmers. The present study investigated microscopic and molecular identification of ticks and tick‐borne anaplasmosis infesting sheep and goats in three agro‐ecological zones of Pakistan. The collected ticks were identified at species level based on their morphological features through microscopy by using taxonomic keys. Four <jats:italic>Rhipicephalus (R.)</jats:italic> species namely: <jats:italic>R. guilhoni Rh. geigyi</jats:italic>, <jats:italic>R. annulatus</jats:italic>, <jats:italic>R. pulchellus</jats:italic>, two <jats:italic>Haemaphysalis (Hae)</jats:italic> spp. <jats:italic>Hae. sulcate</jats:italic>, <jats:italic>Hae. punctate</jats:italic>, one <jats:italic>Ixodes ricinus and Hyalomma anatolicum</jats:italic> were identified. The morphological variations in their body sizes, scutal pattern, female genital opening, spiracular plate length and male adanal plate were recorded. Furthermore, we assessed the effectiveness of utilizing the 16S rRNA gene sequence to differentiate <jats:italic>Anaplasma</jats:italic> samples at the species level. Microscopically suspected positive blood samples for <jats:italic>Anaplasma</jats:italic> were subjected to DNA extraction. <jats:italic>Anaplasma</jats:italic> was observed in the blood of 14 animals. The sequence similarity between <jats:italic>Anaplasma marginale</jats:italic> and the reference genome were 99.25%, while below this value indicating a distinct species. Phylogenetic analysis revealed close similarity with <jats:italic>A. marginale</jats:italic> isolates from Iran, Vietnam and Pakistan This study contributes to the epidemiology of ticks and TBPs in the KP province and highlights the importance of proactive TBP surveillance in livestock.\",\"PeriodicalId\":50945,\"journal\":{\"name\":\"Acta Zoologica\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Zoologica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/azo.12510\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Zoologica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/azo.12510","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

蜱虫是吸血寄生虫,会将病原体传播给人类、家畜和野生动物。蜱虫严重危害绵羊和山羊的生产,给农民造成了严重的经济损失。本研究通过显微镜和分子鉴定,对巴基斯坦三个农业生态区域绵羊和山羊中的蜱虫和蜱传无形体病进行了调查。根据蜱虫的形态特征,通过显微镜和分类钥匙对收集到的蜱虫进行了物种鉴定。四种 Rhipicephalus(R:Guilhoni Rh. geigyi、R. annulatus、R. pulchellus,两种 Haemaphysalis(Hae)属 Hae. sulcate、Hae. punctate,一种 Ixodes ricinus 和 Hyalomma anatolicum。我们记录了它们的体型、鳞片形态、雌性生殖器开口、螺纹板长度和雄性腺板的形态变化。此外,我们还评估了利用 16S rRNA 基因序列在物种水平上区分阿纳疟原虫样本的有效性。对显微镜下疑似阿纳疟原虫阳性的血液样本进行 DNA 提取。在 14 只动物的血液中观察到了阿纳普拉丝虫。边缘疽疟原虫与参考基因组的序列相似度为 99.25%,低于该值则表明是一个独特的物种。这项研究有助于研究 KP 省蜱虫和 TBPs 的流行病学,并强调了对牲畜进行前瞻性 TBP 监测的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Insights into tick dynamics and anaplasmosis in ruminants: A microscopic and molecular perspective
Ticks are blood‐sucking parasite and transmit pathogens to humans, domestic and wild animals. Ticks are seriously damage the production of sheep and goats, resulting in heavy economic loss to farmers. The present study investigated microscopic and molecular identification of ticks and tick‐borne anaplasmosis infesting sheep and goats in three agro‐ecological zones of Pakistan. The collected ticks were identified at species level based on their morphological features through microscopy by using taxonomic keys. Four Rhipicephalus (R.) species namely: R. guilhoni Rh. geigyi, R. annulatus, R. pulchellus, two Haemaphysalis (Hae) spp. Hae. sulcate, Hae. punctate, one Ixodes ricinus and Hyalomma anatolicum were identified. The morphological variations in their body sizes, scutal pattern, female genital opening, spiracular plate length and male adanal plate were recorded. Furthermore, we assessed the effectiveness of utilizing the 16S rRNA gene sequence to differentiate Anaplasma samples at the species level. Microscopically suspected positive blood samples for Anaplasma were subjected to DNA extraction. Anaplasma was observed in the blood of 14 animals. The sequence similarity between Anaplasma marginale and the reference genome were 99.25%, while below this value indicating a distinct species. Phylogenetic analysis revealed close similarity with A. marginale isolates from Iran, Vietnam and Pakistan This study contributes to the epidemiology of ticks and TBPs in the KP province and highlights the importance of proactive TBP surveillance in livestock.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta Zoologica
Acta Zoologica 生物-动物学
CiteScore
2.90
自引率
8.30%
发文量
35
审稿时长
>12 weeks
期刊介绍: Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.
期刊最新文献
Issue Information Immune system of fish with special reference to estrogenic immune regulation: A review Otolith‐based species identification in the killifish Aphaniops (Teleostei; Cyprinodontiformes; Aphaniidae) using both morphometry and wavelet analysis Not all heroes wear shells: New data on the spicules' morphology in acochlidimorph mollusc Development, differentiation and toughness in lizard claws derive from two types of cysteine–glycine‐rich proteins
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1