基于羟酰胺螯合物的新型锝-99m 标记二价 PSMA 靶向探针用于诊断前列腺癌。

IF 2.5 4区 医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Annals of Nuclear Medicine Pub Date : 2024-07-08 DOI:10.1007/s12149-024-01959-9
Yoichi Shimizu, Masato Ando, Hiroyuki Watanabe, Masahiro Ono
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引用次数: 0

摘要

目的:前列腺特异性膜抗原(PSMA前列腺特异性膜抗原(PSMA)是众所周知的前列腺癌生物标志物。此前,我们的研究小组曾报道,琥珀酰亚胺基-胱抑素-脲-谷氨酸(SCUE)分子与 PSMA 有很高的亲和力。本研究中,我们开发了新型锝-99m 标记 PSMA 靶向探针"[99mTc]Tc-(Ham-SCUE)2",该探针基于含二价 SCUE 的羟酰胺螯合物,并评估了其作为 SPECT 成像探针诊断表达 PSMA 的前列腺癌的潜力。将[99mTc]Tc-(Ham-SCUE)2加入LNCaP(PSMA高表达)细胞或PC3(PSMA低表达)细胞,60分钟后测量其放射性。阻断研究是在加入[99mTc]Tc-(Ham-SCUE)2 之前,先将 LNCaP 细胞与不同浓度的 2-PMPA(一种 PSMA 抑制剂)共孵育 15 分钟。静脉注射[99mTc]Tc-(Ham-SCUE)2 120 分钟后,评估了[99mTc]Tc-(Ham-SCUE)2 在 LNCaP/PC3 双异种移植 C.B.-17/Icr scid/scid Jcl 小鼠体内的生物分布情况。阻断研究是通过对小鼠进行 2-PMPA(10 毫克/千克体重)预处理进行的:结果:[99m锝]Tc-(Ham-SCUE)2的放射化学收率为56%,放射化学纯度超过95%。LNCaP细胞对[99mTc]Tc-(Ham-SCUE)2的摄取水平明显高于PC3细胞(LNCaP:11.12 ± 0.71 vs. PC3:1.40 ± 0.13%uptake/mg protein,2-PMPA的p 50为245 ± 47 nM)。在体内研究中,给予[99mTc]Tc-(Ham-SCUE)2 120 分钟后,LNCaP 肿瘤组织的放射性明显高于 PC3 肿瘤组织(LNCaP:9.97 ± 2.79,PC3:1.16 ± 0.23%ID/g,p 结论:[99mTc]Tc-(Ham-SCUE)2 对 PC3 肿瘤组织的放射性没有影响:[99mTc]Tc-(Ham-SCUE)2 有潜力成为诊断高 PSMA 表达前列腺癌的 SPECT 成像剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Novel technetium-99m-labeled bivalent PSMA-targeting probe based on hydroxamamide chelate for diagnosis of prostate cancer

Objective

Prostate-specific membrane antigen (PSMA) is a well-known biomarker of prostate cancer. Previously, our group reported that the succinimidyl–cystatin–urea–glutamate (SCUE) moiety has a high affinity for PSMA. In this study, we developed the novel technetium-99m-labeled PSMA-targeting probe “[99mTc]Tc-(Ham-SCUE)2” based on a hydroxamamide chelate with a bivalent SCUE and evaluated its potential as a SPECT imaging probe for the diagnosis of PSMA-expressing prostate cancer.

Methods

Ham-SCUE was synthesized by a one-step reaction with Ham-Mal and cysteine-urea-glutamine. Then, Ham-SCUE was reacted with [99mTc]NaTcO4 for 10 min at room temperature to obtain [99mTc]Tc-(Ham-SCUE)2. [99mTc]Tc-(Ham-SCUE)2 was added to LNCaP (high PSMA expression) cells or PC3 (low PSMA expression) cells, and their radioactivity was measured 60 min after administration. The blocking study was performed by co-incubation of LNCaP cells with various concentrations of 2-PMPA (a PSMA inhibitor) for 15 min before adding [99mTc]Tc-(Ham-SCUE)2. The biodistribution of [99mTc]Tc-(Ham-SCUE)2 in LNCaP/PC3 dual xenografted C.B.-17/Icr scid/scid Jcl mice was evaluated for 120 min after intravenous injection. The blocking study was performed by pretreatment of mice with 2-PMPA (10 mg/kg weight).

Results

[99mTc]Tc-(Ham-SCUE)2 was acquired at radiochemical yields of 56% with a radiochemical purity of over 95%. The cellular uptake level of [99mTc]Tc-(Ham-SCUE)2 by LNCaP cells was significantly higher than that by PC3 cells (LNCaP: 11.12 ± 0.71 vs. PC3: 1.40 ± 0.13%uptake/mg protein, p < 0.01), and the uptake was significantly suppressed by pretreatment with 2-PMPA (2.56 ± 0.37%uptake/mg protein, p < 0.05). IC50 of 2-PMPA was 245 ± 47 nM. In the in vivo study, the radioactivity of LNCaP tumor tissue was significantly higher than that of PC3 tumor tissue at 120 min after the administration of [99mTc]Tc-(Ham-SCUE)2 (LNCaP: 9.97 ± 2.79, PC3: 1.16 ± 0.23%ID/g, p < 0.01), and was suppressed by pretreatment with 2-PMPA (2.50 ± 0.45%ID/g, p < 0.01).

Conclusion

[99mTc]Tc-(Ham-SCUE)2 has the potential to be a SPECT imaging agent for diagnosing high PSMA-expressing prostate cancer.

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来源期刊
Annals of Nuclear Medicine
Annals of Nuclear Medicine 医学-核医学
CiteScore
4.90
自引率
7.70%
发文量
111
审稿时长
4-8 weeks
期刊介绍: Annals of Nuclear Medicine is an official journal of the Japanese Society of Nuclear Medicine. It develops the appropriate application of radioactive substances and stable nuclides in the field of medicine. The journal promotes the exchange of ideas and information and research in nuclear medicine and includes the medical application of radionuclides and related subjects. It presents original articles, short communications, reviews and letters to the editor.
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