病理红细胞的光学鉴别

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioengineering Pub Date : 2024-07-07 DOI:10.1002/bit.28798

Michele Vergari, Benedetta Niccolini, Dario Pitocco, Alessandro Rizzi, Gabriele Ciasca, Marco de Spirito, Luca Gavioli

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引用次数: 0

摘要

快速诊断方法对于减轻医疗系统的负担至关重要。目前，检测神经病变等糖尿病并发症需要耗费大量时间来观察相关红细胞（RBC）的形态变化。为了解决这个问题，我们在 250-2500 纳米范围内对空气中的红细胞进行了光学分析。利用米氏理论和反常衍射近似法分析了散射和直接透射光谱中存在的明显振荡。结果提供了有关 RBC 末端膨胀的信息，并将光学数据与 RBC 形态和变形性直接联系起来。两种模型都认为糖尿病患者的红细胞体积和变形能力减小，从而为糖尿病诊断和疾病进展评估开辟了道路。

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Optical discrimination of pathological red blood cells

Fast diagnostic methods are crucial to reduce the burden on healthcare systems. Currently, detection of diabetes complications such as neuropathy requires time-consuming approaches to observe the correlated red blood cells (RBCs) morphological changes. To tackle this issue, an optical analysis of RBCs in air was conducted in the 250–2500 nm range. The distinct oscillations present in the scattered and direct transmittance spectra have been analyzed with both Mie theory and anomalous diffraction approximation. The results provide information about the swelling at the ends of RBCs and directly relate the optical data to RBCs morphology and deformability. Both models agree on a reduction in the size and deformability of RBCs in diabetic patients, thus opening the way to diabetes diagnosis and disease progression assessment.

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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