线粒体生物能与肌纤维蛋白质合成率无关。

IF 9.4 1区 医学 Q1 GERIATRICS & GERONTOLOGY Journal of Cachexia Sarcopenia and Muscle Pub Date : 2024-07-15 DOI:10.1002/jcsm.13532
Andrew M. Holwerda, Marlou L. Dirks, Pierre-Andre Barbeau, Joy Goessens, Annemie Gijsen, Luc J.C. van Loon, Graham P. Holloway
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引用次数: 0

摘要

背景:线粒体是影响细胞平衡的关键细胞器,与调节蛋白质合成的信号事件有关:我们研究了在体外透化肌纤维中测量的线粒体生物能(氧化磷酸化和活性氧(H2O2)释放,ROS)是否代表了肌肉蛋白质合成率和骨骼肌质量在各种体力活动(包括自由生活和卧床休息)中变化的调节因素:n = 19 名健康的年轻男性(26 ± 4 岁,23.4 ± 3.3 kg/m2)和 12 周阻力型运动训练后:n = 10 名健康老年男性(70 ± 3 岁,25.2 ± 2.1 kg/m2)。此外,我们还评估了线粒体 ROS 排放减少与转基因小鼠(线粒体靶向过氧化氢酶,MCAT)每日肌纤维和肌浆蛋白质合成率之间的直接关系:在自由生活条件下或卧床休息 1 周后,氧化磷酸化和 H2O2 排放均与健康年轻男性的肌肉蛋白质合成率无关(P 均 > 0.05)。GSSG浓度的增加与卧床休息后骨骼肌质量的减少有关(r = -0.49,P 2O2 排放与训练后骨骼肌质量的变化无关(均为 P > 0.05)。此外,与野生型小鼠相比,MCAT 小鼠的肌纤维(2.62 ± 0.22 vs. 2.75 ± 0.15%/天)和肌浆(3.68 ± 0.35 vs. 3.54 ± 0.35%/天)蛋白质合成率没有差异(均为 P > 0.05):结论:线粒体氧化磷酸化和活性氧释放似乎并不是调节肌肉蛋白质合成或肌肉质量的关键因素。
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Mitochondrial bioenergetics are not associated with myofibrillar protein synthesis rates

Background

Mitochondria represent key organelles influencing cellular homeostasis and have been implicated in the signalling events regulating protein synthesis.

Methods

We examined whether mitochondrial bioenergetics (oxidative phosphorylation and reactive oxygen species (H2O2) emission, ROS) measured in vitro in permeabilized muscle fibres represent regulatory factors for integrated daily muscle protein synthesis rates and skeletal muscle mass changes across the spectrum of physical activity, including free-living and bed-rest conditions: n = 19 healthy, young men (26 ± 4 years, 23.4 ± 3.3 kg/m2) and following 12 weeks of resistance-type exercise training: n = 10 healthy older men (70 ± 3 years, 25.2 ± 2.1 kg/m2). Additionally, we evaluated the direct relationship between attenuated mitochondrial ROS emission and integrated daily myofibrillar and sarcoplasmic protein synthesis rates in genetically modified mice (mitochondrial-targeted catalase, MCAT).

Results

Neither oxidative phosphorylation nor H2O2 emission were associated with muscle protein synthesis rates in healthy young men under free-living conditions or following 1 week of bed rest (both P > 0.05). Greater increases in GSSG concentration were associated with greater skeletal muscle mass loss following bed rest (r = −0.49, P < 0.05). In older men, only submaximal mitochondrial oxidative phosphorylation (corrected for mitochondrial content) was positively associated with myofibrillar protein synthesis rates during exercise training (r = 0.72, P < 0.05). However, changes in oxidative phosphorylation and H2O2 emission were not associated with changes in skeletal muscle mass following training (both P > 0.05). Additionally, MCAT mice displayed no differences in myofibrillar (2.62 ± 0.22 vs. 2.75 ± 0.15%/day) and sarcoplasmic (3.68 ± 0.35 vs. 3.54 ± 0.35%/day) protein synthesis rates when compared with wild-type mice (both P > 0.05).

Conclusions

Mitochondrial oxidative phosphorylation and reactive oxygen emission do not seem to represent key factors regulating muscle protein synthesis or muscle mass regulation across the spectrum of physical activity.

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来源期刊
Journal of Cachexia Sarcopenia and Muscle
Journal of Cachexia Sarcopenia and Muscle MEDICINE, GENERAL & INTERNAL-
CiteScore
13.30
自引率
12.40%
发文量
234
审稿时长
16 weeks
期刊介绍: The Journal of Cachexia, Sarcopenia and Muscle is a peer-reviewed international journal dedicated to publishing materials related to cachexia and sarcopenia, as well as body composition and its physiological and pathophysiological changes across the lifespan and in response to various illnesses from all fields of life sciences. The journal aims to provide a reliable resource for professionals interested in related research or involved in the clinical care of affected patients, such as those suffering from AIDS, cancer, chronic heart failure, chronic lung disease, liver cirrhosis, chronic kidney failure, rheumatoid arthritis, or sepsis.
期刊最新文献
Issue Information Neuromuscular impairment at different stages of human sarcopenia The impact of mobility limitations on geriatric rehabilitation outcomes: Positive effects of resistance exercise training (RESORT) Artificial neural network inference analysis identified novel genes and gene interactions associated with skeletal muscle aging Hydrogen sulfide inhibits skeletal muscle ageing by up-regulating autophagy through promoting deubiquitination of adenosine 5’-monophosphate (AMP)-activated protein kinase α1 via ubiquitin specific peptidase 5
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