从婴儿粪便中分离的植物乳杆菌 ZFM518 的益生特性和全基因组序列分析

IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Food frontiers Pub Date : 2024-07-07 DOI:10.1002/fft2.444
Yingjuan Zhang, Qingqing Zhou, Ziqi Chen, Zhongdu Ye, Ying Jin, Ping Li, Qing Gu
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引用次数: 0

摘要

新近的研究表明,新生儿肠道中的乳酸菌对婴儿出生后的生长、免疫功能和新陈代谢起着有益的作用。本研究从新生儿粪便样本中分离出四株乳酸菌,经过筛选获得了安全的植物乳杆菌 ZFM518(ZFM518)菌株,该菌株表现出卓越的抗菌活性和粘附潜力。该菌株分别在酸性环境、模拟胃液和模拟肠道环境中表现出了极佳的存活能力。ZFM518 对金黄色葡萄球菌的抑菌区为 31.12 ± 0.33 mm,疏水率为 76.97% ± 3.35%,在模拟肠液环境下的存活率为 96.54% ± 0.14%。此外,ZFM518 可产生高达 161.11 ± 9.67 纳克/毫升的叶酸。对 ZFM518 的全基因组调查显示,其大部分基因参与氨基酸、碳水化合物、粘附、免疫防御和抗菌活性的代谢。此外,该抗菌肽只有一个抗生素耐药基因被注释。这些结果表明,ZFM518 是一株综合能力强、具有益生潜力的新菌株。这项研究可为从婴儿粪便中筛选潜在的益生菌提供实际支持,并为开发益生菌资源提供理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Probiotic characteristics and whole genome sequence analysis of Lactiplantibacillus plantarum ZFM518 isolated from infant feces

Emerging research has shown that lactic acid bacteria in the intestines of newborns play a beneficial role in the growth, immune function, and metabolism of infants after birth. In this study, four strains of Lactobacillus were isolated from fecal samples of newborns, and a safe Lactiplantibacillus plantarum ZFM518 (ZFM518) strain was obtained after the screening, which showed excellent antibacterial activity and adhesion potential. The strain exhibited excellent ability to survive in acidic environments, simulated gastric juice, and simulated intestinal environments, respectively. ZFM518 had a bacteriostatic zone of 31.12 ± 0.33 mm against Staphylococcus aureus, a hydrophobic rate of 76.97% ± 3.35%, and a survival activity of 96.54% ± 0.14% under a simulated intestinal fluid environment. Moreover, ZFM518 can produce up to 161.11 ± 9.67 ng/mL of folate. A genome-wide investigation of ZFM518 revealed that the majority of its genes were involved in the metabolism of amino acids, carbohydrates, adhesion, immunological defense, and antibacterial activity. In addition, only one antibiotic resistance gene of the antimicrobial peptide was annotated. These results indicate that ZFM518 is a new strain with a strong comprehensive ability and probiotic potential. This study can provide practical support for screening potential probiotics from infant feces and provide a theoretical basis for developing probiotic resources.

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来源期刊
CiteScore
10.50
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0.00%
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审稿时长
10 weeks
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