使用注射器分离人体外周血制备血小板丰富血浆 (PRP) 的可行性研究

Youhe Tang, Xinyuan Tang, Mengjun Wang
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摘要

本文主要探讨富血小板血浆(PRP)的新制备方法,以提高其临床应用和普及率。作为一种天然生长因子,PRP 在促进组织修复和缓解炎症方面具有显著优势,但制备方法的局限性阻碍了其广泛应用。本研究使用临床医用注射器作为制备 PRP 的离心容器。在不破坏血液分层的前提下,该方法采用了一种推动技术,使密度梯度离心后的血液通过一个医用三通阀,分离出不同的血液成分,从而得到目标 PRP 层。这种方法操作简单,回收效率较高,并能确保在整个过程中在封闭环境中处理血液,减少环境污染。它可以在普通环境下进行。作者寻求一种可行、易操作、对环境要求低、成本低的分离方法,以促进 PRP 在临床上的普及。
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Feasibility Study on Using Syringes to Separate Human Peripheral Blood for Preparing Platelet-Rich Plasma (PRP)
This paper focuses on exploring new methods for preparing platelet-rich plasma (PRP) to improve its clinical application and popularization. As a natural growth factor, PRP has significant advantages in promoting tissue repair and alleviating inflammation, but limitations in preparation methods hinder its widespread use. The study uses clinical medical syringes as the centrifuge container for PRP preparation. Without disrupting the stratification of the blood, the method employs a pushing technique to move the density gradient centrifuged blood through a medical three-way valve, separating the different blood components to obtain the target PRP layer. This method is simple to operate, has a higher recovery efficiency, and ensures the blood is handled in a closed environment throughout the process, reducing environmental contamination. It can be performed in ordinary settings. The authors seek a feasible, easy-to-operate, environmentally low-requirement, and low-cost separation method to facilitate the clinical popularization of PRP.
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